Isolation and purification of stevioside from stevia leaves

• Autorzy: Joshi Jignesh , Gautam Alok , Gautam Shina

Identyfikatory

DOI

10.24425/cpe.2022.140822

• Konferencja: The International Chemical Engineering Conference 2021 (ICHEEC): 100 Glorious Years of Chemical Engineering and Technology, September 16–19, 2021
• Języki publikacji: EN

Abstrakty

EN

Stevia rebaudiana Bertoni which has gained industrial and scientific interests is a suitable nutritional alternative to sucrose as a sweetener. Recently, there have been studies which show the extraction of this phytochemical substance from stevia leaves and purification methods by several alcohols and chromatographic methods. However, these methods are not cost-effective. Therefore, an attempt was made to extract and purify ST using inexpensive, scalable and simple techniques where different steps like extraction, electrocoagulation, ion exchange, activated charcoal, vacuum evaporation and butanol wash were used as purification steps. The present study established a new improved technology of extraction of ST from stevia leaves using water as a solvent followed by various purification steps. 496 mg of Stevioside extracted in the form of crystals was obtained from 100 g of leaves which is 10 times more than the reported yield of 54 mg from 100 g stevia leaves in literature. This methodology can be scaled up at the industry level for future large production to meet the huge demand for natural sweeteners.

Słowa kluczowe

EN

ST; rebaudioside-A; Stevia rebaudiana Bertoni; conventional extraction

PL

ST; rebaudiozyd-A; Stevia Rebaudiana Bertoni; ekstrakcja konwencjonalna

• Wydawca: Komitet Inżynierii Chemicznej i Procesowej Polskiej Akademii Nauk
• Czasopismo: Chemical and Process Engineering
• Rocznik: 2022
• Tom: Vol. 43, nr 2
• Strony: 193–--202
• Opis fizyczny: Bibliogr. 21 poz., tab., rys.

Twórcy

autor

Joshi Jignesh

• Chemical Engineering Dept, Government Engineering College, Valsad, Gujarat, India-382424
• Gujarat Technological University, Chandkheda, Ahmedabad, 382424 Gujarat, India

autor

Gautam Alok

• Chemical Engineering Dept, Shroff S. R. Rotary Institute Of Chemical Technology, Bharuch 393135, Gujarat, India
• Gujarat Technological University, Chandkheda, Ahmedabad, 382424 Gujarat, India

autor

Gautam Shina

• Chemical Engineering Dept, Shroff S. R. Rotary Institute Of Chemical Technology, Bharuch 393135, Gujarat, India
• Gujarat Technological University, Chandkheda, Ahmedabad, 382424 Gujarat, India

Bibliografia

• 1. Akashi H., Yokoyama Y., Osada M., 1975. Extraction and purification of steviosides. Japan Kokai, 75(24), 300.
• 2. Anton S. D., Martin C.K., Han H., Coulon S., Cefalu W.T., Geiselman P., Williamson D.A., 2010. Effects of stevia, aspartame, and sucrose on food intake, satiety, and postprandial glucose and insulin levels. Appetite, 55, 37–43. DOI: 10.1016/j.appet.2010.03.009.
• 3. Chranioti C., Chanioti S., Tzia C., 2016. Comparison of spray, freeze and oven drying as a means of reducing bitter aftertaste of steviol glycosides (derived from Stevia rebaudiana Bertoni plant) – Evaluation of the final products.
• 4. Food Chem., 190, 1151–1158. DOI: 10.1016/j.foodchem.2015.06.083. Crammer B., Ikan R., 1977. Properties and syntheses of sweetening agents. Chem. Soc. Rev., 6, 431–465. DOI: 10.1039/CS9770600431.
• 5. Formigoni M., Milani P.G., da Silva Avíncola A., dos Santos V.J., Benossi L., Dacome A.S., Pilau E.J., da Costa S.C., 2018. Pretreatment with ethanol as an alternative to improve steviol glycosides extraction and purification from a new variety of stevia. Food Chemistry, 241, 452–459. DOI: 10.1016/j.foodchem.2017.09.022.
• 6. Ghosh S., Subudhi E., Nayak S., 2008. Antimicrobial assay of Stevia rebaudiana Bertoni leaf extracts against 10 pathogens. Int. J. Integr. Biol., 2(1), 27–31.
• 7. Gomez L., Tiwari B., Garcia-Vaquero M., 2020. Chapter 9 - Emerging extraction techniques: Microwave-assisted extraction, In: Torres M.D., Kraan S., Dominguez H. (Eds.), Advances in green and sustainable chemistry. Sustainable seaweed technologies, 207-224. Elsevier. DOI: 10.1016/B978-0-12-817943-7.00008-1.
• 8. Goyal S.K., Samsher G.R., Goyal R.K., 2010. Stevia (Stevia rebaudiana) a bio-sweetener: a review. Int. J. Food Sci. Nutr., 61, 1–10. DOI: 10.3109/09637480903193049.
• 9. Hidayat R., Wulandari P.J.E.H.I., 2021. Methods of extraction: maceration, percolation and decoction. Eureka Herba Indonesia, 2, 68–74. DOI: 10.37275/ehi.v2i1.15.
• 10. Huang X.-Y., Fu J.-F., Di D.-L., 2010. Preparative isolation and purification of steviol glycosides from Stevia rebaudiana Bertoni using high-speed counter-current chromatography. Sep. Purif. Technol., 71, 220–224. DOI: 10.1016/j.seppur.2009.11.025.
• 11. Ishizone H., 1979. Separation and purification of stevioside. Japan Kokai Tokkyo Koho, 79(12), 400. Jumpatong K., Phutdhawong W., Buddhasukh D.J.M., 2006. Dechlorophyllation by electrocoagulation. Molecules, 11, 156–162. DOI: 10.3390/11020156.
• 12. Kaur G., 2009. Biochemical composition and characterization of glycosides of stevia rebaudiana bertoni at different development stages. MSc Thesis, Punjab Agricultural University, Ludhiana.
• 13. Kumari N., Rana R., Sharma Y., Kumar S., 2017. Extraction, purification and analysis of sweet compounds in Stevia rebaudiana Bertoni using chromatographic techniques. Indian J. Pharm. Sci., 79, 617–624. DOI: 10.4172/pharmaceutical-sciences.1000270.
• 14. Lee C.-N., Wong K.-L., Liu J.-C., Chen Y.-J., Cheng,J.-T., Chan P., 2001. Inhibitory effect of stevioside on calcium influx to produce antihypertension. Planta Med., 67, 796–799. DOI: 10.1055/s-2001-18841.
• 15. Megeji N., Kumar J., Singh V., Kaul V., Ahuja P.S., 2005. Introducing Stevia rebaudiana, a natural zero-caloriesweetener. Current Science, 88, 801–804.
• 16. Savita S., Sheela K., Sunanda S., Shankar A., Ramakrishna P., 2004. Stevia rebaudiana – A functional component for food industry. J. Hum. Ecol., 15, 261–264. DOI: 10.1080/09709274.2004.11905703.
• 17. Singh S., Rao G., 2005. Stevia: The herbal sugar of 21𝑠𝑡 century. Sugar Tech., 7, 17–24. DOI: 10.1007/BF02942413.
• 18. Takahashi K., Matsuda M., Ohashi K., Taniguchi K., Nakagomi O., Abe Y., Mori S., Sato N., Okutani K., Shigeta S., 2001. Analysis of anti-rotavirus activity of extract from Stevia rebaudiana. Antiviral Res., 49, 15–24. DOI: 10.1016/S0166-3542(00)00134-0.
• 19. Weihrauch M., Diehl V., 2004. Artificial sweeteners – do they bear a carcinogenic risk? Ann. Oncol., 15, 1460–1465. DOI: 10.1093/annonc/mdh256.
• 20. Xi Y., 1998. Antioxidant mechanism of Stevia rebaudiana extract and antioxidant activity of inorganic salts. Nippon Shokuhin Kagaku Kogaku Kaishi, 45, 317–322. DOI: 10.3136/nskkk.45.317.
• 21. Yadav A.K., Singh S., Dhyani D., Ahuja P.S., 2011. A review on the improvement of stevia [Stevia rebaudiana (Bertoni)]. Can. J. Plant. Sci., 91, 1–27. DOI: 10.4141/cjps10086.