The Prevalence of tet(A) and tet(M) Tetracycline Resistance Genes in Municipal Wastewater

• Autorzy: Hubeny Jakub , Buta Martyna , Zieliński Wiktor , Harnisz Monika , Korzeniewska Ewa , Nowrotek Monika , Płaza Grażyna

Identyfikatory

DOI

10.12911/22998993/112714

• Języki publikacji: EN

Abstrakty

EN

Antibiotic resistance is a widespread problem that poses one of the greatest risks to public health around the world. The main cause of antibiotic resistance is the overuse of antibiotics in the human and veterinary medicine and in agriculture. Drugs are released into the environment with treated wastewater, and they can act as stressors that increase the prevalence of antibiotic resistance genes (ARGs). Wastewater treatment plants (WWTPs) are not equipped with appropriate technologies for eliminating the genetic material from the treated wastewater. In this study, the prevalence of tet(A) and tet(M) genes encoding resistance to tetracycline antibiotics was investigated in the samples of municipal wastewater and sewage sludge collected from two WWTPs and in the water samples collected from rivers which receive the treated wastewater. The samples were collected in two seasons of the year (summer and fall). The presence of ARGs was confirmed by PCR. The study revealed that ARGs were not effectively removed from wastewater by the WWTP in the Region of Silesia. Seasonal variations in the occurrence of the analyzed genes were not observed in the samples collected from the above-mentioned plant. Tetracycline resistance genes were detected in all samples of river water. The tet(A) gene was not removed from the treated wastewater in the WWTP in the Region of Warmia and Mazury, whereas the tet(M) gene was detected on a seasonal basis. The tet(M) gene was not detected in the samples of river water collected upstream and downstream from the WWTP. The study demonstrated that the existing WWTPs lack the means to eliminate ARGs. The wastewater treatment systems have to be modified to effectively remove ARGs from the treated wastewater.

Słowa kluczowe

EN

WWTP; tetracyclines; ARGs; ARB; antibiotic resistance; environment; wastewater

• Wydawca: Polskie Towarzystwo Inżynierii Ekologicznej ; Lublin University of Technology
• Czasopismo: Journal of Ecological Engineering
• Rocznik: 2019
• Tom: Vol. 20, nr 10
• Strony: 1--6
• Opis fizyczny: Bibliogr. 34 poz., tab.

Twórcy

autor

Hubeny Jakub

• Department of Environmental Microbiology, University of Warmia and Mazury, ul. Prawocheńskiego 1, 10-720, Olsztyn, Poland

autor

Buta Martyna

• Department of Environmental Microbiology, University of Warmia and Mazury, ul. Prawocheńskiego 1, 10-720, Olsztyn, Poland

autor

Zieliński Wiktor

• Department of Environmental Microbiology, University of Warmia and Mazury, ul. Prawocheńskiego 1, 10-720, Olsztyn, Poland

autor

Harnisz Monika

• Department of Environmental Microbiology, University of Warmia and Mazury, ul. Prawocheńskiego 1, 10-720, Olsztyn, Poland

autor

Korzeniewska Ewa

• Department of Environmental Microbiology, University of Warmia and Mazury, ul. Prawocheńskiego 1, 10-720, Olsztyn, Poland

autor

Nowrotek Monika

• Department of Environmental Microbiology, Institute for Ecology of Industrial Areas, ul. Kossutha 6, 40-844 Katowice, Poland

autor

Płaza Grażyna

• Department of Environmental Microbiology, Institute for Ecology of Industrial Areas, ul. Kossutha 6, 40-844 Katowice, Poland

Bibliografia

• 1. Boto L. 2014. Horizontal gene transfer in the acquisition of novel traits by metazoans. Proceedings of the Royal Society B: Biological Sciences, 281(1777), 20132450.
• 2. Chen G., Zhao L., & Dong Y. H. 2011. Oxidative degradation kinetics and products of chlortetracycline by manganese dioxide. Journal of hazardous materials, 193, 128–138.
• 3. Chen H., & Zhang M. 2013. Occurrence and removal of antibiotic resistance genes in municipal wastewater and rural domestic sewage treatment systems in eastern China. Environment international, 55, 9–14.
• 4. D’Costa V. M., King C. E., Kalan L., Morar M., Sung W. W., Schwarz C. & Golding G. B. 2011. Antibiotic resistance is ancient. Nature, 477(7365), 457.
• 5. Daghrir R., & Drogui P. 2013. Tetracycline antibiotics in the environment: a review. Environmental chemistry letters, 11(3), 209–227.
• 6. Deblonde T., Cossu-Leguille C., & Hartemann P. 2011. Emerging pollutants in wastewater: a review of the literature. International journal of hygiene and environmental health, 214(6), 442–448.
• 7. Gao P., Munir M., & Xagoraraki I. 2012. Correlation of tetracycline and sulfonamide antibiotics with corresponding resistance genes and resistant bacteria in a conventional municipal wastewater treatment plant. Science of the Total Environment, 421, 173–183.
• 8. Ghosh S., & LaPara T. M. 2007. The effects of subtherapeutic antibiotic use in farm animals on the proliferation and persistence of antibiotic resistance among soil bacteria. The ISME journal, 1(3), 191.
• 9. Guo J., Li J., Chen H., Bond P. L., & Yuan Z. 2017. Metagenomic analysis reveals wastewater treatment plants as hotspots of antibiotic resistance genes and mobile genetic elements. Water research, 123, 468–478.
• 10. Harnisz M., & Korzeniewska E. 2018. The prevalence of multidrug-resistant Aeromonas spp. in the municipal wastewater system and their dissemination in the environment. Science of the Total Environment, 626, 377–383.
• 11. Harnisz M., Korzeniewska E., Ciesielski S., & Gołaś I. 2015. tet genes as indicators of changes in the water environment: Relationships between culture
• 12. He L. Y., Ying G. G., Liu Y. S., Su H. C., Chen J., Liu S. S., & Zhao J. L. 2016. Discharge of swine wastes risks water quality and food safety: Antibiotics and antibiotic resistance genes from swine sources to the receiving environments. Environment international, 92, 210–219. dependent and culture-independent approaches. Science of the Total Environment, 505, 704–711.
• 13. Jeong J., Song W., Cooper W. J., Jung J., & Greaves J. 2010. Degradation of tetracycline antibiotics: mechanisms and kinetic studies for advanced oxidation/reduction processes. Chemosphere, 78(5), 533–540.
• 14. Korzeniewska E., Korzeniewska A., & Harnisz M. 2013. Antibiotic resistant Escherichia coli in hospital and municipal sewage and their emission to the environment. Ecotoxicology and environmental safety, 91, 96–102.
• 15. Kümmerer K. 2009. Antibiotics in the aquatic environment–a review–part I. Chemosphere, 75(4), 417–434.
• 16. Laht M., Karkman A., Voolaid V., Ritz C., Tenson T., Virta M., & Kisand V. 2014. Abundances of tetracycline, sulphonamide and beta-lactam antibiotic resistance genes in conventional wastewater treatment plants (WWTPs) with different waste load. PLoS One, 9(8), e103705.
• 17. Li R., Zhang Y., Lee C. C., Liu L., & Huang Y. 2011. Hydrophilic interaction chromatography separation mechanisms of tetracyclines on amino-bonded silica column. Journal of separation science, 34(13), 1508–1516.
• 18. Michael I., Rizzo L., McArdell C. S., Manaia C. M., Merlin C., Schwartz T. & Fatta-Kassinos D. 2013. Urban wastewater treatment plants as hotspots for the release of antibiotics in the environment: a review. Water research, 47(3), 957–995.
• 19. Moura A., Henriques I., Smalla K., & Correia A. 2010. Wastewater bacterial communities bring together broad-host range plasmids, integrons and a wide diversity of uncharacterized gene cassettes. Research in microbiology, 161(1), 58–66.
• 20. Nawaz M., Sung K., Khan S. A., Khan A. A., & Steele R. 2006. Biochemical and molecular characterization of tetracycline-resistant Aeromonas veronii isolates from catfish. Appl. Environ. Microbiol., 72(10), 6461–6466.
• 21. Ng L. K., Martin I., Alfa M., & Mulvey M. 2001. Multiplex PCR for the detection of tetracycline resistant genes. Molecular and cellular probes, 15(4), 209–215.
• 22. Park C., & Zhang J. 2012. High expression hampers horizontal gene transfer. Genome biology and evolution, 4(4), 523–532
• 23. Reijnders D., Goossens G. H., Hermes G. D., Neis E. P., van der Beek C. M., Most J., & Groen A. K. (2016). Effects of gut microbiota manipulation by antibiotics on host metabolism in obese humans: a randomized double-blind placebo-controlled trial. Cell metabolism, 24(1), 63–74.
• 24. Rizzo L., Manaia C., Merlin C., Schwartz T., Dagot C., Ploy M. C., & Fatta-Kassinos D. 2013. Urban wastewater treatment plants as hotspots for antibiotic resistant bacteria and genes spread into the environment: a review. Science of the total environment, 447, 345–360.
• 25. Schlüter A., Szczepanowski R., Pühler A., & Top E. M. 2007. Genomics of IncP-1 antibiotic resistance plasmids isolated from wastewater treatment plants provides evidence for a widely accessible drug resistance gene pool. FEMS microbiology reviews, 31(4), 449–477.
• 26. Soucy S. M., Huang J., & Gogarten J. P. 2015. Horizontal gene transfer: building the web of life. Nature Reviews Genetics, 16(8), 472.
• 27. von Wintersdorff,C. J., Penders J., van Niekerk J. M., Mills N. D., Majumder S., van Alphen L. B., & Wolffs P. F. 2016. Dissemination of antimicrobial resistance in microbial ecosystems through horizontal gene transfer. Frontiers in microbiology, 7, 173.
• 28. World Health Organization. 2014. Antimicrobial resistance: global report on surveillance. World Health Organization. (ISBN: 9241564741).
• 29. Xiong W., Sun Y., Zhang T., Ding X., Li Y., Wang M., & Zeng Z. 2015. Antibiotics, antibiotic resistance genes, and bacterial community composition in fresh water aquaculture environment in China. Microbial ecology, 70(2), 425–432.
• 30. Xu J., Xu Y., Wang H., Guo C., Qiu H., He Y. & Meng W. 2015. Occurrence of antibiotics and antibiotic resistance genes in a sewage treatment plant and its effluent-receiving river. Chemosphere, 119, 1379–1385.
• 31. Zhang T., & Li B. 2011. Occurrence, transformation, and fate of antibiotics in municipal wastewater treatment plants. Critical reviews in environmental science and technology, 41(11), 951–998.
• 32. Zhang X. X., & Zhang T. 2011. Occurrence, abundance, and diversity of tetracycline resistance genes in 15 sewage treatment plants across China and other global locations. Environmental science & technology, 45(7), 2598–2604.
• 33. Zhang X. X., Zhang T., & Fang H. H. 2009. Antibiotic resistance genes in water environment. Applied microbiology and biotechnology, 82(3), 397–414.
• 34. Zhu Y. G., Johnson T. A., Su J. Q., Qiao M., Guo G. X., Stedtfeld R. D. & Tiedje J. M. 2013. Diverse and abundant antibiotic resistance genes in Chinese swine farms. Proceedings of the National Academy of Sciences, 110(9), 3435–3440.