Cytotoxicity Elicited by Molybdenum Disulphide in Different Size of Particles in Human Airway Cells

• Autorzy: Zapór Lidia

Warianty tytułu

PL

Ocena cytotoksycznego działania cząstek disiarczku molibdenu na komórki układu oddechowego

• Języki publikacji: EN

Abstrakty

EN

The present investigation was aimed to study the cytotoxic response induced by molybdenum disulfide in nano- (MoS₂-NPs) and micro- (MoS₂-MPs) size of particle in human bronchial (BEAS-2B) and alveolar (A549) cells. The cells were exposed with different particle size of MoS₂ in concentrations range 1-200 μg/mL for 24, 48, and 72 h, and then the cytotoxicity assays (MTT and NRU) was performed. Afterwards, long-term toxicity was assessed by colony forming efficiency assay (CFEA) during 10 days exposure of the cells. Both MoS₂-NPs and MoS₂-MPs showed similar, weak cytotoxic effects on BEAS-2B and A549 cells assessed by MTT assay, that is reduction of cell viability to approx. 60-70% at concentrations of 2.5 and 5 μg/mL. The percentage viability remained relatively constant at this level across all concentrations above 5 μg/mL. In long-term exposure, both MoS₂ inhibited colony formation in a wider range of concentrations to 200 μg/mL. MoS₂-NPs were slightly more cytotoxic than MoS₂-MPs. The data suggest the low potential hazardous nature of both MoS₂ tested with an indication of higher toxicity of MoS₂-NPs.

PL

Celem badań była ocena cytotoksycznego działania nano- (MoS₂-NPs) i mikrometrycznego (MoS₂-MPs) disiarczku molibdenu na ludzkie komórki nabłonka oskrzelików (BEAS-2B) i pęcherzyków (A549) płuc. Komórki narażano na różnej wielkości cząstki MoS₂ w zakresie stężeń 1-200 μg/ml przez 24, 48 i 72 h. Badano wpływ obu rodzaju cząstek MoS₂ na integralność błon komórkowych (test NRU) oraz aktywność metaboliczną komórek (test MTT). Oceniano również zdolność komórek do proliferacji po długotrwałym (10 dni) narażeniu na podstawie testu efektywności formowania kolonii (CFEA). Oba MoS₂ powodowały podobne, słabe działanie cytotoksyczne na komórki oceniane testem MTT, tj. obniżenie przeżywalności komórek do ok. 60-70% w stężeniach of 2,5 and 5 μg/mL po 24 h, które w niewielkim stopniu nasilało się z czasem narażenia, natomiast nie obserwowano wzrostu cytotoksyczności ze wzrostem stężenia. Po długotrwałej ekspozycji (10 dni) oba MoS₂ hamowały zdolność tworzenia kolonii w szerszym zakresie stężeń tj. do 200 μg/mL, przy czym MoS₂-NPs wykazywały silniejsze działanie ograniczające zdolność komórek do proliferacji niż MoS₂-MPs. Dane wskazują na niski potencjał toksyczny obu badanych MoS₂ ze wskazaniem wyższej toksyczności MoS₂-NP.

Słowa kluczowe

EN

molybdenum disulphide; nanoparticles; cytotoxicity

PL

disiarczek molibdenu; nanocząstki; cytotoksyczność

• Wydawca: Politechnika Koszalińska
• Czasopismo: Rocznik Ochrona Środowiska
• Rocznik: 2019
• Tom: Tom 21, cz. 2
• Strony: 794--809
• Opis fizyczny: Bibliogr. 42 poz., rys.

Twórcy

autor

Zapór Lidia

• Central Institute for Labour Protection – National Research Institute (CIOP-PIB), Warsaw, Poland

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Uwagi

Opracowanie rekordu ze środków MNiSW, umowa Nr 461252 w ramach programu "Społeczna odpowiedzialność nauki" - moduł: Popularyzacja nauki i promocja sportu (2020).