In vivo test of acute exposure of polyethylene microplastics on kidney and liver of Rattus norvegicus Wistar strain rats

• Autorzy: Inaku Awaluddin Hidayat Ramli , Hadiyanto Hadiyanto , Abdurachim Henna Rya , Bariroh Tahyatul , Rachmawati Siti

Identyfikatory

DOI

10.12912/27197050/196654

• Języki publikacji: EN

Abstrakty

EN

The increasing use of plastics has caused severe environmental pollution, especially microplastics with plastic particles with a diameter of 5 mm or less. These particles are formed by environmental factors such as weathering and ultraviolet radiation, worsening environmental pollution. This environmental pollution increases human exposure to microplastics through the food chain. Many studies have reported the adverse effects of microplastic exposure on food and aquatic organisms. However, relatively few studies have used white mice. The entry of microplastics into white mice can affect two essential organs, namely the liver and kidneys. In this study, we conducted in-vivo experimental tests on male white mice by exposing them to a single dose of pure PE (polyethylene) type microplastics and then observing them for 14 days. Toxicological effects were evaluated comprehensively in white mice by examining clinical signs, body weight, and kidney and liver function activity through blood tests for creatinine, SGOT, and SGPT parameters. Further kidney and liver abnormalities were analyzed through histopathological tests. Based on the results of experiments carried out, it was found that PE-type microplastics had a significant impact on changes in body weight, increased functional activity of the kidneys and liver in white mice as well as chronic histopathological abnormalities in male white mice, so it is hoped that this research can clarify that PE type microplastics can affect kidney and liver organs for those who consume large amounts in a short time.

Słowa kluczowe

EN

acute; poltethylene; kidney; liver; in vivo

• Wydawca: Lubelski Oddział Polskiego Towarzystwo Inżynierii Ekologicznej ; Lublin University of Technology ; WNGB Wydawnictwo Naukowe
• Czasopismo: Ecological Engineering & Environmental Technology
• Rocznik: 2025
• Tom: Vol. 26, iss. 2
• Strony: 164--177
• Opis fizyczny: Bibliogr. 42 poz., rys., tab.

Twórcy

autor

Inaku Awaluddin Hidayat Ramli

• Department of Doctoral Environmental Science, Faculty of Postgraduate, Diponegoro University, Semarang, 50275, Indonesia
• Department of Public Health, Faculty of Health Sciences, Universitas Muhammadiyah Prof. Dr. Hamka, Jakarta, Indonesia 12210

• https://orcid.org/0009-0004-1916-7000

autor

Hadiyanto Hadiyanto

• Department of Chemical Engineering, Faculty of Engineering, Universitas Diponegoro, Semarang, Indonesia 50275

• https://orcid.org/0000-0003-0074-7078

autor

Abdurachim Henna Rya

• Department of Pharmacy, Faculty of Medical, Universitas Diponegoro, Semarang, 50275, Indonesia

• https://orcid.org/0000-0002-5449-6465

autor

Bariroh Tahyatul

• Pharmacy and Sciences Faculty, Universitas Muhammadiyah Prof. Dr. Hamka, Jakarta, 12210, Indonesia

• https://orcid.org/0000-0003-2327-1690

autor

Rachmawati Siti

• Department of Environmental Science, Faculty of Mathematics and Natural Sciences, Sebelas Maret University, Surakarta, 57126, Indonesia

• https://orcid.org/0000-0001-6566-0374

Bibliografia

• 1. Amato-Lourenço LF, Carvalho-Oliveira R, Júnior GR, Dos Santos Galvão L, Ando RA, Mauad T. (2021). Presence of airborne microplastics in human lung tissue. J Hazard Mater. 15; 416, 126124. https://doi.org/10.1016/j.jhazmat.2021.126124
• 2. Andrady, A.L. (2011). Microplastics in the marine environment, Marine Pollution Bulletin, 62(8), 1596–1605. https://doi.org/10.1016/j. marpolbul.2011.05.030
• 3. Avio, C. G., Gorbi, S., Milan, M., Benedetti, M., Fattorini, D., D’Errico, G., Pauletto, M., Bargelloni, L., & Regoli, F. (2015). Pollutants bioavailability and toxicological risk from microplastics to marine mussels. Environmental Pollution, 198, 211–222. https://doi.org/10.1016/j.envpol.2014.12.021
• 4. Banerjee, A. and Shelver, W.L. (2021). Micro- and nanoplastic induced cellular toxicity in mammals: A review, Science of the Total Environment, 755, 142518. https://doi.org/10.1016/j.scitotenv.2020.142518
• 5. Besseling, E., Wang, B., Lürling, M., Koelmans, A.A. 2021. Nanoplastic affects growth of S. obliquus and reproduction of D. magna. Environ Sci Technol. 20, 12336–43. https://doi.org/10.1021/es503001d
• 6. Browne, M.A, Niven, S.J., Galloway, T.S., Rowland, S.J., Thompson, R.C. (2013). Microplastic moves pollutants and additives to worms, reducing functions linked to health and biodiversity. Curr Biol. 23(23), 2388–92. https://doi.org/10.1016/j. cub.2013.10.012
• 7. Cole, M., Lindeque, P., Fileman, E., Halsband, C., Galloway, T.S. (2015). The impact of polystyrene microplastics on feeding, function and fecundity in the marine copepod Calanus helgolandicus, Environmental Science and Technology, 49(2), 1130– 1137. https://doi.org/10.1021/es504525u
• 8. Deng, Y., Zhang, Y., Lemos, B., Ren, H. (2017). Tissue accumulation of microplastics in mice and biomarker responses suggest widespread health risks of exposure, Scientific Reports, 7(March), 1–10. https://doi.org/10.1038/srep46687
• 9. Goodman, K.E., Hua, T. and Sang, Q.X.A. (2022). Effects of polystyrene microplastics on human kidney and liver cell morphology, cellular proliferation, and metabolism, ACS Omega, 7(38), 34136–34153. Available at: https://doi.org/10.1021/ acsomega.2c03453
• 10. Guo, J.J., Huang, X.P., Xiang, L., Wang, Y.Z., Li, Y.W., Li, H., Cai, Q.Y., Mo, C.H., Wong, M.H. (2020). Source, migration and toxicology of microplastics in soil, Environment International, 137, 105263. https://doi.org/10.1016/j.envint.2019.105263
• 11. Hämer, J., Gutow, L., Köhler, A., Saborowski, R. (2014). Fate of microplastics in the marine isopod idotea emarginata, Environmental Science and Technology, 1–15.
• 12. Horvatits, T., Tamminga, M., Liu, B., Sebode, M., Carambia, A., Fischer, L., Püschel, K., Huber, S., Fischer, E.K. (2022). Microplastics detected in cirrhotic liver tissue, eBioMedicine, 82(22), 1–21. https://doi.org/10.1016/j.ebiom.2022.104147
• 13. Hou, B., Wang, F., Liu, T., Wang, Z. (2021).Reproductive toxicity of polystyrene microplastics: In vivo experimental study on testicular toxicity in mice, Journal of Hazardous Materials, 405(June), 124028. https://doi.org/10.1016/j.jhazmat.2020.124028
• 14. Jannah, D.R. and Budijastuti, W. (2022). Gambaran Histopatologi Toksisitas Ginjal Tikus Jantan (Rattus norvegicus) yang Diberi Sirup Umbi Yakon (Smallanthus sonchifolius) Histopatological Overview Kidneys Toxicity of A Male Rat (Rattus norvegicus) Being Given Yakon Tuber Smallanthis sonc’, Lentera Bio, 11(2), 238–246.
• 15. Kik, K., Bukowska, B. and Sicińska, P. (2020). Polystyrene nanoparticles: Sources, occurrence in the environment, distribution in tissues, accumulation and toxicity to various organisms, Environmental Pollution, 262. https://doi.org/10.1016/j. envpol.2020.114297
• 16. Leslie H.A., van Velzen M.J.M., Brandsma S.H., Vethaak A.D., Garcia-Vallejo J.J., Lamoree M.H. (2022). Discovery and quantification of plastic particle pollution in human blood, Environment International, 163, 107199. https://doi.org/10.1016/j. envint.2022.107199
• 17. Lu, F., Langenbacher, A.D. and Chen, J.N. (2016). Transcriptional regulation of heart development in zebrafish, Journal of Cardiovascular Development and Disease, 3(2). https://doi.org/10.3390/ jcdd3020014
• 18. Luís, L.G., Ferreira, P., Fonte, E., Oliveira, M., Guilhermino, L. (2015). Does the presence of microplastics influence the acute toxicity of chromium(VI) to early juveniles of the common goby (Pomatoschistus microps)? A study with juveniles from two wild estuarine populations, Aquatic Toxicology, 164, 163– 174. https://doi.org/10.1016/j.aquatox.2015.04.018
• 19. Mattsson, K., Ekvall, M.T., Hansson, L.A., Linse, S., Malmendal, A., Cedervall, T. (2015). Altered behavior, physiology, and metabolism in fish exposed to polystyrene nanoparticles, Environmental Science and Technology, 49(1), 553–561. https://doi.org/10.1021/es5053655
• 20. Nakamura, J.S., Kim, E.S., Rentscher, K.E., Bower, J.E., Kuhlman, K.R. (2022). Early-life stress, depressive symptoms, and inflammation: the role of social factors., Aging & mental health, 26(4), 843–851. https://doi.org/10.1080/13607863.2021.1876636
• 21. Nelms, S.E., Galloway, T.S., Godley, B.J., Jarvis, D.S., Lindeque, P.K. (2018). Investigating microplastic trophic transfer in marine top predators, Environmental Pollution, 238, 999–1007. https://doi.org/10.1016/j.envpol.2018.02.016
• 22. Nugrahapraja, H., Sugiyo, P.W.W., Putri, B.Q., Ni’matuzahroh, F., Huang, L., Hafza, N., Götz, F., Santoso, H., Wibowo, A.T., & Luqman, A. (2022). Effects of microplastic on human gut microbiome: detection of plastic-degrading genes in human gut exposed to microplastics—preliminary study, Environments - MDPI, 9(11). https://doi.org/10.3390/environments9110140
• 23. Nurfaat, D.L. and Indriyati, W. (2016). Acute toxicity test of ethanol extract of mango misletoe (Dendrophthoe petandra) to Strain of Swiss Webster Mice, Ijpst, 3(2).
• 24. Opinion, S. (2011). Scientific opinion on epizootic ulcerative syndrome, EFSA Journal, 9(10). https://doi.org/10.2903/j.efsa.2011.2387
• 25. Paloian, N.J. and Giachelli, C.M. (2014). A current understanding of vascular calcification in CKD., American journal of physiology. Renal physiology, 307(8), F891–900. https://doi.org/10.1152/ajprenal.00163.2014
• 26. Prata, J.C., da Costa, J.P., Lopes, I., Duarte, A.C., Rocha-Santos, T. (2020). Environmental exposure to microplastics: An overview on possible human health effects., The Science of the total environment, 702, 134455. https://doi.org/10.1016/j.scitotenv.2019.134455
• 27. Ragusa, A., Svelato, A., Santacroce, C., Catalano, P., Notarstefano, V., Carnevali, O., Papa, F., Rongioletti, M.C.A., Baiocco, F., Draghi, S., D’Amore, E., Rinaldo, D., Matta, M., Giorgini, E. (2021). Plasticenta: First evidence of microplastics in human placenta, Environment International, 146, 106274. https://doi.org/10.1016/j.envint.2020.106274
• 28. Reddy, M.M., Vivekanandhan, S., Misra, M., Bhatia, S.K., & Mohanty, A.K. (2013). Biobased plastics and bionanocomposites: Current status and future opportunities, Progress in Polymer Science, 38(10–11), 1653–1689. https://doi.org/10.1016/j. progpolymsci.2013.05.006
• 29. Rochman, C.M., Kurobe, T., Flores, I., The, S.J. (2014). Early warning signs of endocrine disruption in adult fish from the ingestion of polyethylene with and without sorbed chemical pollutants from the marine environment, Science of the Total Environment, 493, 656–661. https://doi.org/10.1016/j.scitotenv.2014.06.051
• 30. Saha, S., Laforsch, C., Ramsperger, A., & Niebel, D. (2023). Microplastic and dermatological care, Dermatologie, 74(1), 27–33. https://doi.org/10.1007/s00105-022-05035-z
• 31. Shen, T., Zhang, W., Wang, Y., Li, H., Wu, J., Wang, Q., Qin, L., Zhang, L., Liu, C., & Li, R. (2023). Relevant doses on the structure, function, and transcriptome. of the Kidney in Mice. Molecules, 28(20), 7104. https://doi.org/10.3390/molecules28207104
• 32. Sincihu, Y. (2022). Dampak pemberian mikroplastik poliethilen peroral terhadap hitung jenis sel leukosit darah Rattus norvegicus strain Wistar., Medical Technology and Public Health Journal, 6, 1–10. https://doi.org/10.33086/mtphj.v6i1.2611
• 33. Statista Research Departement. (2023). Annual production of plastics worldwide from 1950 to 2021(in million metric tons), Statista, 2021, 24–27.
• 34. de Stephanis, R., Giménez, J., Carpinelli, E., Gutierrez-Exposito, C., Cañadas, A. As main meal for sperm whales: plastics debris. (2013). As main meal for sperm whales: Plastics debris, Marine Pollution Bulletin, 69(1–2), 206–214. https://doi.org/10.1016/j.marpolbul.2013.01.033
• 35. Turner, A.C., Kraev, I., Stewart, M.G., Stramek, A., Overton, P.G., Dommett, E.J. (2018). Chronic amphetamine enhances visual input to and suppresses visual output from the superior colliculus in withdrawal, Neuropharmacology, 138, 118–129. https://doi.org/10.1016/j.neuropharm.2018.05.033
• 36. Uurasjärvi, E., Sainio, E., Setälä, O., Lehtiniemi, M., Koistinen, A. (2021). Validation of an imaging FTIR spectroscopic method for analyzing microplastics ingestion by Finnish lake fish (Perca fluviatilis and Coregonus albula), Environmental Pollution, 288(July). https://doi.org/10.1016/j.envpol.2021.117780
• 37. Vianello, A., Jensen, R.L., Liu, L., Vollertsen, J. (2019). Simulating human exposure to indoor airborne microplastics using a Breathing Thermal Manikin, Scientific Reports, 9(1), 1–11. https://doi.org/10.1038/s41598-019-45054-w
• 38. Wahyuni, F.S., Putri, I.N. and Arisanti, D. (2017). Uji toksisitas subkronis fraksi etil asetat kulit buah asam kandis (Garcinia cowa Roxb) terhadap Fungsi Hati dan Ginjal Mencit Putih Betina, Jurnal Sains Farmasi & Klinis, 3(2), 202. https://doi.org/10.29208/jsfk.2017.3.2.126
• 39. Watts, A.J., Lewis, C., Goodhead, R.M., Beckett, S.J., Moger, J., Tyler, C.R., Galloway, T.S. (2014). Uptake and retention of microplastics by the shore crab carcinus maenas, Environmental Science and Technology, 48(15), 8823–8830. https://doi.org/10.1021/es501090e
• 40. Wright, S.L. and Kelly, F.J. (2017). Plastic and human health: a micro issue?, Environmental science & technology, 51(12), 6634–6647. https://doi.org/10.1021/acs.est.7b00423
• 41. Yee, M.S., Hii, L.W., Looi, C.K., Lim, W.M., Wong, S.F., Kok, Y.Y., Tan, B.K., Wong, C.Y., Leong, C.O. (2021). Impact of microplastics and nanoplastics on human health, Nanomaterials, 11(2), 1–23. https://doi.org/10.3390/nano11020496
• 42. Zhang, Z., Chen, W., Chan, H., Peng, J., Zhu, P., Li, J., Jiang, X., Zhang, Z., Wang, Y., Tan, Z., Peng, Y., Zhang, S., Lin, K., Yung, K.K. (2024). Polystyrene microplastics induce size-dependent multi-organ damage in mice: Insights into gut microbiota and fecal metabolites, Journal of Hazardous Materials, 461(January), 1–6. https://doi.org/10.1016/j.jhazmat.2023.132503