Simultaneous determination of atractylenolide I and II in rat plasma by UPLC–MS/MS and its application to pharmacokinetic study after intravenous administration

• Autorzy: Chen Lianguo , Wu Haiya , Tu Xiaoting , Zhao Yi , Jiang Yanyan , Wen Congcong , Luo Yue

Identyfikatory

DOI

10.1556/1326.2017.00274

• Języki publikacji: EN

Abstrakty

EN

Atractylodis exerted a variety of pharmacological effects such as anti-tumor, anti-inflammatory, anti-bacterial, and anti-aging effects etc. The major ingredients of Atractylodis are atractylenolide I and II that exhibited activities in anti-inflammatory and anticancer. In this work, a sensitive and selective ultra-performance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS) method for determination of atractylenolide I and II in rat plasma was developed. The UPLC–MS/MS method was validated for selectivity, linearity, accuracy, precision, recovery, and stability with a total run time of 4.0 min. After addition of atractylenolide III as an internal standard (IS), protein precipitation by acetonitrile was used to prepare samples. Chromatographic separation was achieved on a UPLC BEH C18 column (2.1 mm × 50 mm, 1.7 μm) with 0.1% formic acid and acetonitrile as the mobile phase with gradient elution. An electrospray ionization source was applied and operated in positive ion mode; multiple reactions monitoring (MRM) mode was used for quantification using target fragment ions m/z 231.1 → 185.1 for atractylenolide I, m/z 233.1 → 91.0 for II, and m/z 249.0 → 231.1 for IS. Calibration plots were linear throughout the range 1–1000 ng/mL for atractylenolide I and II in rat plasma. Mean recoveries of atractylenolide I and II in rat plasma ranged from 86.2% to 96.3%. Relative standard deviation (RSD) of intra-day and inter-day precision was both less than 12%. The accuracy of the method was between 91.0% and 109.0%. The method was successfully applied to pharmacokinetic study of atractylenolide I and II after intravenous administration in rats.

Słowa kluczowe

EN

Atractylenolide I; atractylenolide II; UPLC–MS/MS; pharmacokinetics; plasma

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2019
• Tom: Vol. 31, no. 1
• Strony: 8--11
• Opis fizyczny: Bibliogr. 20 poz., rys., tab.

Twórcy

autor

Chen Lianguo

• Department of Pharmacy, Wenzhou People's Hospital, Wenzhou, China

autor

Wu Haiya

• The Second Affiliated Hospital and Yuying Children's Hospital, Wenzhou Medical University, Wenzhou, China

autor

Tu Xiaoting

• Laboratory Animal Centre, Wenzhou Medical University, Wenzhou, China

autor

Zhao Yi

• Laboratory Animal Centre, Wenzhou Medical University, Wenzhou, China

autor

Jiang Yanyan

• Laboratory Animal Centre, Wenzhou Medical University, Wenzhou, China

autor

Wen Congcong

• Laboratory Animal Centre, Wenzhou Medical University, Wenzhou, China

autor

Luo Yue

• The Second Affiliated Hospital and Yuying Children's Hospital, Wenzhou Medical University, Wenzhou, China

Bibliografia

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Uwagi

PL

Opracowanie rekordu w ramach umowy 509/P-DUN/2018 ze środków MNiSW przeznaczonych na działalność upowszechniającą naukę (2019).