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Simultaneous determination of atractylenolide I and II in rat plasma by UPLC–MS/MS and its application to pharmacokinetic study after intravenous administration

Identyfikatory
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
Atractylodis exerted a variety of pharmacological effects such as anti-tumor, anti-inflammatory, anti-bacterial, and anti-aging effects etc. The major ingredients of Atractylodis are atractylenolide I and II that exhibited activities in anti-inflammatory and anticancer. In this work, a sensitive and selective ultra-performance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS) method for determination of atractylenolide I and II in rat plasma was developed. The UPLC–MS/MS method was validated for selectivity, linearity, accuracy, precision, recovery, and stability with a total run time of 4.0 min. After addition of atractylenolide III as an internal standard (IS), protein precipitation by acetonitrile was used to prepare samples. Chromatographic separation was achieved on a UPLC BEH C18 column (2.1 mm × 50 mm, 1.7 μm) with 0.1% formic acid and acetonitrile as the mobile phase with gradient elution. An electrospray ionization source was applied and operated in positive ion mode; multiple reactions monitoring (MRM) mode was used for quantification using target fragment ions m/z 231.1 → 185.1 for atractylenolide I, m/z 233.1 → 91.0 for II, and m/z 249.0 → 231.1 for IS. Calibration plots were linear throughout the range 1–1000 ng/mL for atractylenolide I and II in rat plasma. Mean recoveries of atractylenolide I and II in rat plasma ranged from 86.2% to 96.3%. Relative standard deviation (RSD) of intra-day and inter-day precision was both less than 12%. The accuracy of the method was between 91.0% and 109.0%. The method was successfully applied to pharmacokinetic study of atractylenolide I and II after intravenous administration in rats.
Rocznik
Strony
8--11
Opis fizyczny
Bibliogr. 20 poz., rys., tab.
Twórcy
autor
  • Department of Pharmacy, Wenzhou People's Hospital, Wenzhou, China
autor
  • The Second Affiliated Hospital and Yuying Children's Hospital, Wenzhou Medical University, Wenzhou, China
autor
  • Laboratory Animal Centre, Wenzhou Medical University, Wenzhou, China
autor
  • Laboratory Animal Centre, Wenzhou Medical University, Wenzhou, China
autor
  • Laboratory Animal Centre, Wenzhou Medical University, Wenzhou, China
autor
  • Laboratory Animal Centre, Wenzhou Medical University, Wenzhou, China
autor
  • The Second Affiliated Hospital and Yuying Children's Hospital, Wenzhou Medical University, Wenzhou, China
Bibliografia
  • [1] Yan, H.; Sun, Y.; Zhang, Q.; Yang, M.; Wang, X.; Wang, Y.; Yu, Z.; Zhao, Y. J. Chromatogr. B: Anal. Technol. Biomed. Life Sci. 2015, 993–994, 86–92.
  • [2] Song, J.; Zheng, S. R.; Jin, Y.; Li, J. Planta. Med. 2014, 80, 187–192.
  • [3] Chen, Q.; He, H.; Li, P.; Zhu, J.; Xiong, M. Biomed. Chromatogr. 2013, 27, 699–707.
  • [4] Long, F.; Wang, T.; Jia, P.; Wang, H.; Qing, Y.; Xiong, T.; He, M.; Wang, X. Med. Sci. Monit. 2017, 23, 571–579.
  • [5] Liu, H.; Zhang, G.; Huang, J.; Ma, S.; Mi, K.; Cheng, J.; Zhu, Y.; Zha, X.; Huang, W. J. Transl. Med. 2016, 14, 104.
  • [6] Wang, A.; Xiao, Z.; Zhou, L.; Zhang, J.; Li, X.; He, Q. Pharm. Biol. 2016, 54, 146–150.
  • [7] Li, Y.; Zhang, Y.; Wang, Z.; Zhu, J.; Tian, Y.; Chen, B. J. Pharm. Biomed. Anal. 2012, 58, 172–176.
  • [8] Shi, Y. Y.; Guan, S. H.; Tang, R. N.; Tao, S. J.; Guo, D. A. Biomed. Chromatogr. 2012, 26, 1386–1392.
  • [9] Ge, J.; Wang, Y. W.; Lu, X. C.; Sun, X. H.; Gong, F. J. Biomed. Chromatogr. 2007, 21, 299–303.
  • [10] Wang, R.; Wang, G.; Hao, H.; Xie, H.; Zhang, J.; Wu, F. J. Chromatogr. B: Anal. Technol. Biomed. Life Sci. 2006, 831, 36–41.
  • [11] Wen, C.; Wang, S.; Huang, X.; Liu, Z.; Lin, Y.; Yang, S.; Ma, J.; Zhou, Y.; Wang, X. Biomed. Chromatogr. 2015, 29, 1805–1810.
  • [12] Wang, S.; Wu, H.; Geng, P.; Lin, Y.; Liu, Z.; Zhang, L.; Ma, J.; Zhou, Y.; Wang, X.; Wen, C. Biomed. Chromatogr. 2016, 30, 1145–1149.
  • [13] Ma, J.; Wang, S.; Huang, X.; Geng, P.; Wen, C.; Zhou, Y.; Yu, L.; Wang, X. J. Pharm. Biomed. Anal. 2015, 111, 131–137.
  • [14] Wen, C.; Zhang, Q.; He, Y.; Deng, M.; Wang, X.; Ma, J. Acta Chromatogr. 2015, 27, 81–91.
  • [15] Ma, J.; Wang, S.; Huang, X.; Geng, P.; Wen, C.; Zhou, Y.; Yu, L.; Wang, X. J. Chromatogr. B: Anal. Technol. Biomed. Life Sci. 2015, 990, 131–137.
  • [16] Ma, J.; Wang, S.; Zhang, M.; Zhang, Q.; Zhou, Y.; Lin, C.; Lin, G.; Wang, X. Biomed. Chromatogr. 2015, 29, 1203–1212.
  • [17] Wang, X.; Wang, S.; Ma, J.; Ye, T.; Lu, M.; Fan, M.; Deng, M.; Hu, L.; Gao, Z. J. Pharm. Biomed. Anal. 2015, 115, 368–374.
  • [18] Wang, X.; Wang, S.; Lin, F.; Zhang, Q.; Chen, H.; Wang, X.; Wen, C.; Ma, J.; Hu, L. J. Chromatogr. B: Anal. Technol. Biomed. Life Sci. 2015, 983–984, 125–131.
  • [19] Wang, S.; Wu, H.; Huang, X.; Geng, P.; Wen, C.; Ma, J.; Zhou, Y.; Wang, X. J. Chromatogr. B: Anal. Technol. Biomed. Life Sci. 2015, 990, 118–124.
  • [20] Zhang, Q.; Wen, C.; Xiang, Z.; Ma, J.; Wang, X. J. Pharm. Biomed. Anal. 2014, 90, 134–138.
Uwagi
PL
Opracowanie rekordu w ramach umowy 509/P-DUN/2018 ze środków MNiSW przeznaczonych na działalność upowszechniającą naukę (2019).
Typ dokumentu
Bibliografia
Identyfikator YADDA
bwmeta1.element.baztech-154039e4-279a-4179-a59e-440e34b6198e
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