Design of experiments and Derringer’s desirability function in optimisation and validation of RP-HPLC method for the analysis of enrofloxacin and its impurities

• Autorzy: Dokmanovic Jelena , Kasagic-Vujanovic Irena , Gagic Žarko , Nikolic Katarina , Carapic Marija , Agbaba Danica

Identyfikatory

DOI

10.1556/1326.2022.01111

• Języki publikacji: EN

Abstrakty

EN

Using the Design of Experiments methodology (Response-Surface Methodology and Derringer’s Desirability Function), a simple, fast and robust RP-HPLC method was developed for the analysis of enrofloxacin (EFC), its impurity A (fluoroquinolonic acid, FQ) and impurity B (ciprofloxacin, CPX). Gradient elution of samples was performed on a Zorbax Eclipse XDB C18 column (15034.6 mm, 3.5 μm) with a mobile phase consisting of 32mM phosphate buffer pH 3.5 – methanol (0 min-19.6% methanol; 15.5 min-19.6% methanol; 29.5 min-80% methanol; 30 min-19.6% methanol; 35 min-19.6% methanol), delivered at a flow rate of 1.5 mL min1, wavelength of detection 278 nm (for EFX and CFX) and 265 nm for FQ. A good linear response was achieved in the range 15–35 μgmL1 (EFX) and LOQ150% for impurities (CFX and FQ). Other validation parameters were also tested: precision, accuracy, sensitivity and robustness. The developed method was shown to be simple, practical and suitable for the analysis of EFC and its impurities (CPX, FQ) in veterinary drugs.

Słowa kluczowe

EN

enrofloxacin; RP-HPLC; design of experiments; Derringer’s desirability function; response surface methodology; optimisation method

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2024
• Tom: Vol. 36, no. 2
• Strony: 132--142
• Opis fizyczny: Bibliogr. 21 poz., rys., wykr.

Twórcy

autor

Dokmanovic Jelena

• Faculty of Pharmacy, University of Belgrade, Belgrade, Serbia

autor

Kasagic-Vujanovic Irena

• Department of Pharmacy, Faculty of Medicine, University of Banja Luka, Banja Luka, Bosnia and Herzegovina

• https://orcid.org/0000-0001-7196-4008

autor

Gagic Žarko

• Department of Pharmacy, Faculty of Medicine, University of Banja Luka, Banja Luka, Bosnia and Herzegovina

autor

Nikolic Katarina

• Faculty of Pharmacy, University of Belgrade, Belgrade, Serbia

autor

Carapic Marija

• Medicines and Medical Devices Agency of Serbia, Belgrade, Serbia

autor

Agbaba Danica

• Faculty of Pharmacy, University of Belgrade, Belgrade, Serbia

Bibliografia

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