Separation and purification of rebaudioside A from extract of Stevia Rebaudiana leaves by macroporous adsorption resins

• Autorzy: Anvari M. , Khayatian G.

Identyfikatory

DOI

10.1515/pjct-2016-0019

• Języki publikacji: EN

Abstrakty

EN

The separation and purification of rebaudioside A from Stevia rebaudiana crude extracts (Steviosides) by macroporous resin were optimized by Taguchi orthogonal array (OA) experimental design methodology. This approach was applied to evaluate the influence of five factors (adsorption temperature, desorption time, elution solution ratio, adsorption volume and type of resin) on the rebaudioside A yield. The percentage contribution of each factor was also determined. The results showed that elution solution ratio and adsorption volume made the greatest (59.6%) and the lowest (1.3%) contribution, respectively. The results showed that the Taguchi method is able to model the purification of rebaudioside A process well (R2 > 0.998) and can therefore be applied in future studies conducted in various fields. Adsorption temperature 35°C, desorption time 60min, elution solution ratio 3, adsorption volume 200ml and HPD-400 as resin were the best conditions determined by the Taguchi method.

Słowa kluczowe

EN

rebaudioside A; Stevia rebaudiana; design of experimental; taguchi orthogonal array

• Wydawca: West Pomeranian University of Technology. Publishing House
• Czasopismo: Polish Journal of Chemical Technology
• Rocznik: 2016
• Tom: Vol. 18, nr 1
• Strony: 127--132
• Opis fizyczny: Bibliogr. 20 poz., rys., tab.

Twórcy

autor

Anvari M.

• Islamic Azad University, Department of Biology, Faculty of Sciences, Rasht Branch, Rasht, Iran

autor

Khayatian G.

• University of Guilan, Department of Chemical Engineering, Technical Faculty, P.O. Box 41635-3756, Rasht, Iran

Bibliografia

• 1. Liu, J., Li, J. & Tang, J. (2010). Ultrasonically assisted extraction of total carbohydrates from Stevia rebaudiana Bertoni and identification of extracts. Food Bioprod. Process. 88, 215–221. DOI: 10.1016/j.fbp.2009.12.005.
• 2. Wheeler, A., Boileau, A.C., Winkler, P.C., Compton, J.C., Prakash, I., Jiang, X. & Mandarino, D.A. (2008). Pharmacokinetics of Rebaudioside A and stevioside after single oral doses in healthy men. Food Chem. Toxicol. 46, 54–60. DOI: 10.1016/j.fct.2008.04.041.
• 3. Geuns, J.M.C. (2003). Molecules of interest stevioside. Phytochemistry 64, 913–921. DOI: 10.1016/S0031-9422(03)00426-6.
• 4. Chatsudthipong, V. & Muanprasat, C. (2009). Stevioside and related compounds: Therapeutic benefits beyond sweetness. Pharmacol. Therapeu. 21, 41–54. DOI: 10.1016/j.pharmthera.2008.09.007.
• 5. Liu, C., Li, L.S., Xu, L.L. & Zhou, Z.M. (2007). Separation and identification of stevioside and Rebaudiodside A in Stevia by HPLC. Chinese J. An. Lab. 26, 23–26.
• 6. Zhang, Y., Chen, T.H., Shi, Z.Q. & He, B.L. (1998). Studies on the separation of Rebaudioside A by recrystallization. Ion Ex. Adsorp. 14, 515–520.
• 7. Babic, K., Van der Ham, L. & De Haan, A.B. (2006). Recovery of benzaldehyde from aqueous streams using extractant impregnated resins. React. Funct. Polym. 66, 1494–1505. DOI: 10.1016/j.reactfunctpolym.2006.04.013.
• 8. Liu, G.M., Zheng, S.R., Yin, D.Q., Xu, Z.Y., Fan, J. & Jiang, F. (2006). Adsorption of aqueous alkylphenol ethoxylate surfactants by mesoporous carbon CMK-3. J. Colloid Interface Sci. 302, 47–53. DOI: 10.1016/j.jcis.2006.06.006.
• 9. Khayati, G. (2013). Optimization of Propionic Acid Extraction by Aqueous Two-Phase System Using Response Surface Methodology. Chem. Eng. Comm. 200, 667–677. DOI: 10.1080/00986445.2012.721032.
• 10. Khayati, G. & Kiyani, F. (2012). A statistical approach for optimization of lipase production by using rice straw: analysis of different inducers and nitrogen sources effect. Minerva Biotecnol. 24, 83–89.
• 11. Anvari, M. & Khayati, G. (2009). Optimization of 2,3-Butanediol Production by Klebsiella pneumoniae PTCC 1290 Using Taguchi Methodology. Asian J. Chem. 21, 2131–2140.
• 12. Behnajady, M.A. Hajiahmadi, M. & Modirshahla, N. (2012). Enhancement of Removal Rate of an Organic Pollutant in the Presence of Immobilized TiO2 Nanoparticles with Inorganic Anions Combination: Optimization Using Taguchi Approach. Ind. Eng. Chem. Res. 51, 15324−15330. DOI: 10.1021/ie301521z.
• 13. Erkucuk, A., Akgun, I.H. & Yesil-Celiktas, O. (2009). Supercritical CO2 extraction of glycosides from Stevia rebaudiana leaves: Identification and optimization. J. Supercrit. Fluid 51, 29–35. DOI: 10.1016/j.supfl u.2009.07.002.
• 14. Li, J., Chen, Z. & Di, D. (2012). Preparative separation and purification of rebaudioside A from Stevia rebaudiana Bertoni crude extracts by mixed bed of macroporous adsorption resins. Food Chem. 132, 268–276. DOI: 10.1016/j. foodchem.2011.10.077.
• 15. Puri, M., Sharma, D., Barrow, C.J. & Tiwary, A.K. (2012). Optimisation of novel method for the extraction of steviosides from Stevia rebaudiana leaves. Food Chem. 132, 1113–1120. DOI: 10.1016/j.foodchem.2011.11.063.
• 16. Fu, X.C., Shen, W.X. & Yao, T.Y. (1990). Physical chemistry. Beijing: Higher Education Press, (Chapter 4), 172.
• 17. Chen, Z., Wei, X., Li, J. & Di, D. (2012). Preparative separation of rebaudioside A from commercialized steviol glycosides by macroporous adsorption resins mixed bed. Sep. Purif. Technol. 89, 22–30. DOI: 10.1016/j.seppur.2012.01.001.
• 18. Xiong, Q., Zhang, Q., Zhang, D., Shi, Y., Jiang, C. & Shi, X. (2014). Preliminary separation and purification of resveratrol from extract of peanut (Arachis hypogaea) sprouts by macroporous adsorption resins. Food Chem. 145, 1–7. DOI: 10.1016/j.foodchem.2013.07.140.
• 19. Wade, J.L.G. (1987). Organic Chemistry, Prentice-Hall, New York, 23–35.
• 20. Joglekar, A.M. & May, A.T. (1987). Product excellence through design of experiments. Cereal Food World 32, 857–868.

Uwagi

Opracowanie ze środków MNiSW w ramach umowy 812/P-DUN/2016 na działalność upowszechniającą naukę.