HPTLC/HPLC and gravimetric methodology for the identification and quantification of gymnemic acid from Gymnema sylvestre methanolic extracts

• Autorzy: Ahmed A. B. A. , Rao A. S. , Rao M. V. , Taha R. M.
• Języki publikacji: EN

Abstrakty

EN

Gymnemic acid (GA), a well known anti-diabetic compound has been detected in methanol extracts of intact leaves and in vitro callus cultures derived from leaf explants of Gymnema sylvestre. Callus biomass was developed in MS medium with optimum plant growth regulators (OPGRs) of 2,4-D (1.5 mg L^-1) + KN (0.5 mg L^-1) under abiotic stress conditions at 45 days determined by growth curve analysis. GA detection and quantification were carried out using thin-layer chromatography (TLC), highperformance thin-layer chromatography (HPTLC), high-performance liquid chromatography (HPLC), and gravimetric techniques. GA detection peak area and their absorption spectra were evaluated through HPTLC and HPLC with the standard GA. Quantification of GA had showed the linearity, accuracy, robustness and precision by HPLC. GA content was significantly higher in gravimetric method than HPLC. All these methods were found to be simple, accurate, selective and rapid and could be successfully applied for the determination of GA. It could have potential as a pharmaceutical drug for Type 1 diabetes mellitus (IDDM) and obesity.

Słowa kluczowe

EN

Gymnema sylvestre; gymnemic acid (GA); abiotic stress; HPTLC; HPLC; gravimetric method

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2013
• Tom: Vol. 25, no. 2
• Strony: 339--361
• Opis fizyczny: Bibliogr. 32 poz., rys., tab.

Twórcy

autor

Ahmed A. B. A.

• Bharathidasan University Department of Plant Science Tiruchirappalli 620 024 Tamil Nadu India
• University of Malaya Institute of Biological Sciences, Faculty of Science 50603 Kuala Lumpur Malaysia

autor

Rao A. S.

• Bharathidasan University Department of Biotechnology Tiruchirappalli 620 024 Tamil Nadu India

autor

Rao M. V.

• Bharathidasan University Department of Plant Science Tiruchirappalli 620 024 Tamil Nadu India

autor

Taha R. M.

• University of Malaya Institute of Biological Sciences, Faculty of Science 50603 Kuala Lumpur Malaysia

Bibliografia

• [1] International Expert Committee, Report on the role of the A1C assay in the diagnosis of diabetes, Diabetes Care, 32, 1–8 (2009)
• [2] ]WHO (2010), http://www.who.int/features/factfiles/obesity/en/
• [3] S.K. Mitra, S. Gopumadhavan, T.S. Muralidhar, S.D. Anturlikar, and M.B. Sujatha, Indian J. Exp. Biol., 33, 798 (1995)
• [4] K.R. Shanmugasundaram, C. Panneerselvam, P. Samudram, and E.R.B. Shanmugasundaram, J. Ethnopharmacol., 7, 205 (1983)
• [5] E.R.B. Shanmugasundaram, K.G. Leela, K.S. Radha, and V.M. Rajendran, J. Ethnopharmacol., 30, 265 (1990)
• [6] Y. Nakamura, Y. Tsumura, Y. Tonogai, and T. Shibata, J. Nutr., 129, 1214 (1999)
• [7] W.C. Ye, Q. Zhang, X. Liu, X. Che, and S. Zhao, Phytochemistry, 53, 893 (2000)
• [8] A. Sokmen, B.M. Jones, and M. Erturk, Phytother. Res. 13, 355 (1999)
• [9] G. Hellekant, D.E. Walters, In: J.A.K Mathlouthi, G.G. Birch (eds), Sweet-Taste Chemoreception, Elsevier, London, 1991, p. 373
• [10] P.V. Kanetkar, R.S. Singhal, K.S. Laddha, and M.Y. Kamat, Phytochem. Anal., 17, 409 (2006)
• [11] C.S. Devi, S. Murugesh, and V.M. Srinivasan, J. Appl. Sci., 6, 2263 (2006)
• [12] E.J. Lee, M. Mobin, E.J. Hahn, and K.Y. Peak, J. Plant Biotech., 49, 427 (2006)
• [13] A.B. Ali Ahmed, A.S. Rao, and M.V. Rao, Methods Mol. Biol., 547, 93 (2009)
• [14] A.B.A. Ahmed, A.S. Rao, M.V. Rao, and R.M. Taha ScientificWorldJournal., 897867 (2012)
• [15] A.B.A. Ahmed, A.S. Rao, and M.V. Rao, Biomedicine, 28, 134 (2008)
• [16] A.B.A. Ahmed, A.S. Rao, and M.V. Rao, Phytomedicine, 17, 1033 (2010)
• [17] T. Murashige and F. Skoog, Physiol. Plant, 15, 473 (1962)
• [18] R.U. Rehman, M. Israr, P.S. Srivastava, K.C. Bansal, and M.Z. Abdin, In Vitro Cell Dev. Biol. Plant, 39, 142 (2003)
• [19] V. Rajpal, Standardization of Botanicals-testing and extraction methods of medicinal herbs, Eastern Publishers, New Delhi, 2002, p. 140
• [20] G. Shin, M. Chil, and D. Lee, Biol. Pharm. Bull., 26, 1321 (2003)
• [21] Smetanska, Adv. Biochem. Eng. Biotech., 111, 197 (2008)
• [22] Y.C. Wang, H.X. Zhang, B. Zhao, and X.F. Yuan, Biotech. Lett., 23, 1971 (2001)
• [23] A. Marston, M. Maillard, and K. Hostettmann, GIT Laboratory J., 1, 36 (1997)
• [24] S.I. Johannessen, D. Battino, D.J. Berry, M. Bialer, G. Kramer, T. Tomson, and P.N. Patsalo, Ther. Drug. Monit., 25, 347 (2003)
• [25] V.S.R. Raju, S. Kannababu, and G.V. Subbraju, Phytochemical Anal., 17, 192 (2006)
• [26] A.B.A. Ahmed, N. Komalavalli, M. Muthukumar, J.H.F. Benjamin, A.S. Rao, S.K. Kim, and M.V. Rao, Comprehensive Bioactive Natural Products-Potential and Challenges, 1, 2009, p. 75
• [27] T. Imoto, F. Yamamoto, A. Miyasaka, and H. Hatano, J. Chromatogr., 557, 383 (1991)
• [28] K. Yoshikawa, K. Amimoto, S. Arihara, and K. Matsuura, Tetrahedron Lett. 30, 1103 (1989)
• [29] Y. Sugihara, H. Nojima, H. Matsuda, T. Murakami, M. Yoshikawa, and I. Kimura, J. Asian Nat. Prod. Res., 2, 321 (2000)
• [30] K. Hostettmann, J.L. Wolfender, and S. Rodriguez, Planta Med., 63, 2 (1997)
• [31] C.S. Joshi, E.S. Priya, and S. Venkataraman, J. Health Sci., 53, 245 (2007)
• [32] A. Bakrudeen Ali Ahmed, A.S. Rao, M.V. Rao, and R.M. Taha, Agro Food Ind Hi Tec., 23, 34 (2012)