Toxicity of Some Phenolic Derivatives : In Vitro Studies

• Autorzy: Zapór L.
• Języki publikacji: EN

Abstrakty

EN

Cytotoxicity of 5 phenol derivatives (phenol, catechol, resorcinol, hydroquinone and phloroglucinol) wastested using a mouse 3T3 fibroblast cell line. Its relationships with structural and physicochemical properties were investigated. Linear regression analysis and Pearson’s correlation coefficient were used to characterise the relationship between cytotoxicity (expressed by IC50 values) and physicochemical parameters of compounds or their toxicity in vivo expressed by LD50 values. The studies showed that physicochemical properties of compounds seemed to have less influence on their cytotoxic potency than structural properties. Cytotoxicity of the compounds probably depends on the number of -OH groups and their location in the aromatic ring more than on physicochemical properties of compounds. The best correlation was obtained for IC50 values and LD50 values determined following rabbit skin administration and experimental skin irritation score.

Słowa kluczowe

EN

phenol; catechol; resorcinol; hydroquinone; phloroglucinol; cytotoxicity

PL

fenol; hydrochinon; badania in vitro; działanie toksyczne; substancje toksyczne; rezorcynol; zagrożenia chemiczne

• Wydawca: Taylor & Francis
• Czasopismo: International Journal of Occupational Safety and Ergonomics
• Rocznik: 2004
• Tom: Vol. 10, No. 4
• Strony: 319--331
• Opis fizyczny: Bibliogr. 38 poz., rys., tab.

Twórcy

autor

Zapór L.

• Department of Chemical and Aerosol Hazards, Central Institute for Labour Protection - National Research Institute, Warsaw, Poland

Bibliografia

• 1. Balls M, Atkinson KA, Gordon VC. Complementation in the development, validation and use of non-animal test batteries, with particular reference to ocular irritancy. ATLA 1991;19:429-31.
• 2. Balls M, Fentem JH. The use of basal cytotoxicity and target organ toxicity tests in hazard identification and risk assessment. ATLA 1992;20:386-88.
• 3. Ekwall B, Clemedson C, Ekwall B, Ring P, Romert L. EDIT: a new international multicentre programme to develop and evaluate batteries of in vitro tests for acute and chronic systemic toxicity. ATLA 1999;27:339-49.
• 4. Seibert H, Balls M, Fentem JH, Bianchi V, Clothier RH, Diericks PJ, Ekwall B, Garle MJ, Gomez-Lechon MJ, Gribaldo L, Gulden M, Liebsch M, Rasmussen R, Shivastava R, Walum E. Acute toxicity testing in vitro and the classification and labelling of chemicals. The report and recommendations of ECVAM Workshop 16. ATLA 1996;24(4):499-510.
• 5. Stark DM, Shopsis C, Borenfreund E, Babich H. Progress and problems in evaluating and validating alternative assays in toxicology. Fd Chem Toxic 1986;24(6/7):449-55.
• 6. Walum E, Balls M, Bianchi V, Blaauboer B, Bolcsfoldi G, Guillouzo A, Moore GA, Odland L, Reinhardt Ch, Spielmann H. ECITTS: an integrated approach to the application of in vitro test systems to the hazard assessment of chemicals. ATLA 1992;20:406-28.
• 7. Barrat MD, Castell JV, Chamberlain M, Combes RD, Dearden JC, Fentem JH, Gerner I, Giuliani A, Gray TJB, Livingstone DJ, McLean Provan W, Rutten FAJL, Verhaar JM, Zbinden P. The integrated use of alternative approaches for predicting toxic hazard. The report and recommendations of ECVAM Workshop 8. ATLA 1995;23:410-9.
• 8. Knight DJ, Breheny D. Alternatives to animal testing in the safety evaluation of products. ATLA 2002:30:7-2.
• 9. Fratello G, Marchini S, Zucco F, Sapora O, Stammati A. Cytotoxicity of halogenated benzenes and its relationship with logP. Toxicol In Vitro 1997;11:673-7.
• 10. Gerner I, Graetschel G, Kahl J, Schlede E. Development of a decision support system for the introduction of alternative methods into local irritancy/corrosivity testing strategies. Development of a relational database. ATLA 2000;28:11-28.
• 11. Jakubowski M. Phenol - documentation. Podstawy i Metody Oceny Środowiska Pracy 2003;35:87-119. In Polish.
• 12. Hayashi M, Nakamura Y, Higashi K, Kato H, Kishida F, Kaneko H. A quantitative structure-activity relationship study of the skin irritation potential of phenols. Toxicol In Vitro 1999;13:915-22.
• 13. Garg R, Kurup A, Hansch C. Comparative QSAR: on the toxicology of the phenolic OH moiety. Crit Rev Toxicol 2001;31(2):223-45.
• 14. Andreoli C, Gigante D, Nunziata A. A review of in vitro methods to assess the biological activity of tobacco smoke with the aim of reducing the toxicity of smoke. Toxicol In Vitro 2003;17:587-94.
• 15. Tennant JA. Evaluation of trypan blue technique for determination of cell viability. Transplantation 1964;12:285-692.
• 16. Borenfreund E, Puerner JA. Toxicity determined in vitro by morphological alterations and neutral red absorption. Toxicol Lett 1985;24:119-24.
• 17. INVITTOX protocol No. 64: The neutral red cytotoxicity assay. Nottingham, UK: INVITTOX; 1992.
• 18. Denizot F, Lang R. Rapid colorimetric assay for cell growth and survival. Modifications to the tetrazolium dye procedure giving improved sensitivity and reliability. J Immunol Methods 1986;89:271-7.
• 19. Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 1983;65:55-63.
• 20. INVITTOX protocol No. 17: MTT assay. Nottingham, UK: INVITTOX; 1990.
• 21. Frazier JM. Multiple endpoint measurements to evaluate the intrinsic cellular toxicity of chemicals. In Vitro 1990;3:349.
• 22. Halle W, Spielmann H. Two procedures for the prediction of acute toxicity (LD50) from cytotoxicity data. ATLA 1992;20(1):40-9.
• 23. Borenfreund E, Babich H, Martin-Alguacil N. Comparison of two in vitro cytotoxicity assays: the neutral red (NR) and tetrazolium MTT tests. Toxicol In Vitro 1990;2:1-6.
• 24. Riddel RJ, Clothier RH, Balls M. An evaluation of three in vitro cytotoxicity assays. Food Chem Toxicol 1986;24:469-71.
• 25. Husoy T, Syversen T, Jenssen J. Comparisons of four in vitro cytotoxicity tests: the MTT assay, NR assay, uridine incorporation and protein measurements. Toxicol In Vitro 1993;7:149-54.
• 26. Chiba K, Kawakami K, Tohyama K. Simultaneous evaluation of cell viability by neutral red, MTT and crystal violet staining assays of the same cells. Toxicol In Vitro 1998;12:251-8.
• 27. Cornelis M, Dupont C, Wepierre J. In vitro cytotoxicity tests on cultured human skin fibroblasts to predict the irritation potential of surfactants. ATLA 1991;19(3):324-36.
• 28. Vian L, Vincent J, Maurin J, Fabre I, Giroux J, Cano JP. Comparison of three cytotoxicity assays for estimating surfactant ocular irritation. Toxicol In Vitro 1995;9:185-90.
• 29. Burch W, Ellinger A, Torok L, Parzafall W, Coulibaly S, Hochegger K, Schorkhuber M, Patrik G, Marian B, Walker R, Sikorska R, Schulte-Hermann L. In vitro studies on subtypes and regulation of active cell death. Toxicol In Vitro 1997;11:579-88.
• 30. Clothier RH, Hulme L, Ahmed AB, Reeves HL, Smith M, Balls M. In vitro cytotoxicity of 150 chemicals to 3T3- L1 cells, assessed by the FRAME kenacid blue method. ATLA 1988;16:84-95.
• 32. Barratt MD. Quantitative structure - activity relationships for skin permeability. Toxicol In Vitro 1995;9:27-37.
• 31. Soffers AEMF, Boersma MG, Vaes WHJ, Vervoort J, Tyrakowska B, Hermens JLM, Rietjens IMCM. Computer-modeling-based QSAR for analyzing experimental data on biotransformation and toxicity. Toxicol In Vitro 2001;15:539-51.
• 33. Zhu X, Rosenkranz HS. Structural basis of the toxicity of chemicals in cultured human HeLa cells. ATLA 2000;25:557-74.
• 34. Babich H, Borenfreund E. Structure-activity relationship (SAR) models established in vitro with the neutral red cytotoxicity assay. Toxicol In Vitro 1987;1:3-9.
• 35. Moss GP, Dearden JC, Patel H, Cronin MTD. Quantitative structure-permeability relationships (QSPRs) for percutaneous absorption. Toxicol In Vitro 2002;16:299-317.
• 36. Knox P, Uphill PF, Fry JR, Benford J, Balls M. The FRAME multicentre project on in vitro cytotoxicology. Food Chem Toxicol 1986;24:457-63.
• 37. Fry JR, Garle MJ, Hammod AH. Choice of acute toxicity measures for comparison of in vivo/in vitro toxicity. ATLA 1988;16:175-9.
• 38. Evans SM, Casartelli A, Herreros E, Minnick DT, Day C, George E, Westmoreland C. Development of a high throughput in vitro toxicity screen predictive of high acute in vivo toxic potential. Toxicol In Vitro 2001;15:579-84.

Uwagi

W bibliografii poz. 31 i poz. 32 mają odwróconą kolejność.