Emulsion electrospinning – method to introduce proteins for biomedical applications

• Autorzy: Kurpanik Roksana , Rapacz-Kmita Alicja , Ścisłowska-Czarnecka Anna , Stodolak-Zych Ewa

Identyfikatory

DOI

10.34821/eng.biomat.162.2021.20-25

• Języki publikacji: EN

Abstrakty

EN

The aim of this work was to obtain polymer fibers by the emulsion electrospinning. For this purpose, polycaprolactone (PCL) was used, which was modified before the electrospinning stage with micelles obtained by the oil-in-water (O/W) emulsion method. Micelles were obtained by combining the non-ionic surfactant Tween 80 or Triton X-100 used at different concentrations with the amino acid alanine. The obtained fibrous substrates had a typical unimodal fiber size distribution and their average size was in the range of 590-800 nm. The effectiveness of the emulsion electrospinning process was confirmed by Fourier Transform Infrared Spectroscopy - Attenuated Total Reflectance (FTIR-ATR) showing the presence of surfactants. The addition of micelles to the polymer solution significantly reduces the contact angle of nonwoven fabrics: from 120° (for PCL) to ~20-30° for surfactant-loaded nonwovens, and the micellar form allows tracking the release of alanine into the solution (UV-Vis). The combination of the core-shell- -morphology of the emulsion electrospun fibers allows comparable amino acid release times. There were no significant differences in both the amount of alanine released and the rate of its release between PCL/ Tween80/alanine and PCL/Triton X-100/alanine fibers, which were characterized by a similar fiber size.

Słowa kluczowe

EN

emulsion electrospinning; alanine; polycaprolactone; Tween 80; Triton X-100; micelle

PL

proteiny; zastosowanie; biomedycyna

• Wydawca: Polish Society for Biomaterials in Cracow
• Czasopismo: Engineering of Biomaterials
• Rocznik: 2021
• Tom: Vol. 24, no. 162
• Strony: 20--25
• Opis fizyczny: Bibliogr. 21 poz., tab., wykr., zdj.

Twórcy

autor

Kurpanik Roksana

• Department of Biomaterials and Composites, Faculty of Materials Science and Ceramics, AGH University of Science and Technology, al. Mickiewicza 30, 30-059 Krakow, Poland

• https://orcid.org/0000-0002-7943-7509

autor

Rapacz-Kmita Alicja

• Department of Ceramics and Refractories, Faculty of Materials Science and Ceramics, AGH University of Science and Technology, al. Mickiewicza 30, 30-059 Krakow, Poland

• https://orcid.org/0000-0001-8788-5144

autor

Ścisłowska-Czarnecka Anna

• Department of Cosmetology, Institute of Applied Sciences, Faculty of Motor Rehabilitation, University of Physical Education in Krakow, al. Jana Pawła II 78, 31-571 Krakow, Poland

• https://orcid.org/0000-0001-8398-8912

autor

Stodolak-Zych Ewa

• Department of Biomaterials and Composites, Faculty of Materials Science and Ceramics, AGH University of Science and Technology, al. Mickiewicza 30, 30-059 Krakow, Poland

• https://orcid.org/0000-0002-8935-4811

Bibliografia

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Uwagi

Opracowanie rekordu ze środków MNiSW, umowa Nr 461252 w ramach programu "Społeczna odpowiedzialność nauki" - moduł: Popularyzacja nauki i promocja sportu (2021).