Biosensory i sensory fluorescencyjne

• Autorzy: Kłos-Witkowska A.

Warianty tytułu

EN

Biosensors and fluorescent sensors

• Języki publikacji: PL

Abstrakty

PL

W pracy opisano podstawy działania biosensorów ze szczególnym uwzględnieniem sensorów fluorescencyjnych. Scharakteryzowano metody fluorescencyjne stosowane w badaniach biosensorów oraz sensorów optycznych. Wyjaśniono różnicę pomiędzy biosensorami a sensorami optycznymi wykorzystującymi zjawisko fluorescencji.. Dokonano zestawienia porfiryn oraz wykrywanych przez nie substancji, a także opisano wykorzystanie sensorów fluorescencyjnych w obrazowaniu nowotworów oraz badaniach nad rozwojem leków.

EN

The paper describes the basics of biosensors with particular emphasis on fluorescent sensors (Figs. 1 and 2). There are characterized fluorescent methods used in the studies of biosensors and optical sensors: a change in fluorescence intensity, FRET (Förster resonance energy transfer) FLIM (Fluorescence life time imaging), FCS (Fluorescence correlation spectroscopy). There is explained the difference between biosensors and optical sensors employing the phenomenon of fluorescence. There are presented optical sensors using porphyrins, paying particular attention to porphyrins as substances characterized by the presence of intense absorption bands in the visible light range, high absorption coefficient and intense emission in the visible and infrared range. Due to these properties they are increasingly used in medical diagnostics for imaging the cancer (photodynamic method), and the stage of cancer (diagnosis based on the emission spectra of protoporphyrin IX). In Table 1 there is given the list of porphyrins and detected by them, on the basis of changes in the fluorescence emission spectra , substances: Fe+3, 1O2, O2, Hg+2 , Ca+2, HCl (gas), phospholipids, Pb+2. There is described the use of fluorescent sensors based on porhyrins for cancer imaging and in cancer drug development studies [15, 16, 17].

Słowa kluczowe

PL

biosensor; sensor optyczny; fluorescencja; porfiryna

EN

biosensor; optical sensor; fluorescence; porphyrin

• Wydawca: Wydawnictwo PAK
• Czasopismo: Pomiary Automatyka Kontrola
• Rocznik: 2014
• Tom: R. 60, nr 1
• Strony: 3--5
• Opis fizyczny: Bibliogr. 21 poz., rys., tab.

Twórcy

autor

Kłos-Witkowska A.

• Akademia Techniczno-Humanistyczna w Bielsku Białej, Wydział Budowy Maszyn i Informatyki, Katedra Elektrotechniki i Automatyki, ul. Willowa 2, 43-309 Bielsko-Biała

Bibliografia

• [1] Morris M. C: Fluorescent biosensors of intracellular targets from genetically encoded reporters to modular polypeptide probes, Cell Biochem Biophys 56, 19-37, 2010.
• [2] Bartoszcze M.: Przegląd Epidemiologiczny 57, 369-376, 2003.
• [3] Tolosa L.: On the design of low- cost fluorescent protein biosensor, Ad Biochem Engin/Biotechnol 116, 143-157, 2009.
• [4] D’Auria S., Lakowicz J.: Enzyme fluorescence as a sensing tool: new perspectives in biotechnology, Curent Opinion in biotechnology, 12: 99-104, 2001.
• [5] VanEngelenburg S., Palmer E.: Fluorescence biosensors of protein function, Curent Opinion in Chemical Biology 12, 60-65, 2008.
• [6] Zadran S., Stanley S., Wong K., Otiniano E., Amighi A., Baudry M.: Fluorescence resonance energy transfer (FRET)-based biosensors: visualizing cellular dynamics and bioenergetics, Applied Microbiology and biotechnology vol. 96, issue 4, 895-902, 2012.
• [7] Duncan R.: Fluorescence lifetime imaging microscopy (FLIM) to quantify protein-protein interaction inside cell, Biochem Soc Trans 34, 679-682, 2006.
• [8] Li I., Pham E., Truong K.: Protein biosensors based on the principle of fluorescence resonance energy transfer for monitoring cellular dynamics, Biotechnol Lett 28, 1971-1982, 2006.
• [9] Dargiewicz- Nowicka J., Radzki S., Chemi I.: Biosensory wykorzystujace porfiryny, Acta Bio-optica et Informatica Medica, vol. 8, 119-131, 2002.
• [10] Legendziewicz J., Gierasymczuk Y., Koll A., Jasiński J.: Fotofizyka układów z ftalocyjaninami i porfirynami oraz perspektywy ich zastosowania, Postępy kosmetologii, 2013.
• [11] Zhong De Liu, Heng Xin Zhao, Cheng Zhi Huang: Obstruction of Photoinduced electron transfer from excited phorpyrin to graphene oxide: A fluorescence turn- on sensing platform for Iron (III) ions, Plos One 7 (12): e50367.doi:10,1371/journal pone.0050367, 2012.
• [12] You M., Wang Y., Wang H., Yang R.: Fluorescent detection of singlet oxygen amplifying signal transduction and improving sensitivity based on intramolecular FRET of anthryl appended porphyrins, vol 556, No. 31, 3253-3259, Nov 2011.
• [13] Yung Z., Tingxiu Y., Haixu Ch., Dapeng H., Tingyao Z., Chunyan He., Xi Ch.: A dissolved oxygen sensor based on composite fluorinated xerogel sensor based on composite fluorinated xerogel dopped with platinum porphyrin dye, Luminescence 26, 29-34, 2011.
• [14] Ibrahim H., Kasselouri A., Raynal B., Pansu R., Prognon P.: Investigating the possible use of a tetra (hydroxyphenyl)porphyrin as a fluorescence probe for the supramolecular detection of phospholipids, Journal of luminescence , vol. 131, issue 12, 2528-2537, 2011.
• [15] Silva F. R. D., Bellini M. H., Tristao V. R., Schor N., Vieira N. D., Courrd L. C.: Intrinsic fluorescence of protoporphyrin IX from blood samples can yield information on the growth of prostate tumours, Journal of fluorescence vol 20, issue 6, page 1159-1165, 2010.
• [16] Silva F. R. D., Nabeshima C. T., Bellini M. H., Schor N., Vieira N. D., Courral L. C.: Study of protoporphyrin IX elimination by Body excreta: A new noninvasive cancer diagnostic method?, Journal of fluorescence vol 23, issue 1, p: 131-135, 2013.
• [17] Friberg E., Cunderlikova B., Pettersen E., Moan J.: pH effects on the cellular uptake of four photosensitizing drugs evaluated for use in photodynamic therapy of cancer, Cancer Letter, vol 195, issue 1, p: 73-80, 2003.
• [18] Yu Yang, Jianhui J, Guoli S, Rugin Yu: An optical sensor for mercury ion based on the fluorescence quenching of tetra (p-dimethylaminophenyl) porphyrin, Analytica Cimica Acta, vol. 636, issue 1, 83-88, 2009.
• [19] Zhou, Li-Jing; Cao, Zhong; Hu, Jing-Lin; Peng, Zhen; Zeng, Pu-Ni; Su, Gang; He, De-Liang: Fluorescent determination of trace calcium in water from high-parameter power plant based on a porphyrin derivative, Advanced Science Letters, vol. 4, p: 1541-1545, 2011.
• [20] Cano M., Castillero P., Roales J., Petrosa J., Britte S., Richardson T., Gonzalez-Elipe A., Barranco A.: A transparent TMPyP/TiO2 composite thin film as an HCl sensitive optochemical gas sensor, Sensors and Actuators B: Chemical, vol. 150, issue 2, p: 764-769, 2010.
• [21] Bozkurt S., Ayata S., Kaynak I: Fluorescence-based sensor for Pb(II) using tetra-(3-bromo-4-hydroxyphenyl)porphyrin in liquid and immobilized medium, Spectochimica Acta Part A: Molecular and Biomolecular Spectroscopy, vol. 72, issue 4, p. 880-883, 2009.