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Simultaneous determination of lycorine and galanthamine in Galanthus woronowii by HPLC-DAD

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Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
Galanthamine, used as a drug for the treatment of Alzheimer’s disease and lycorine possessing important biological properties, are widely distributed alkaloids in the members of Amaryllidaceae family. In the present study, the contents of these alkaloids have been quantitatively determined by high-performance liquid chromatography (HPLC) coupled with diode array detector (DAD) in the aerial parts and bulbs of Galanthus woronowii Losinsk. collected from different localities in Northeastern Turkey. A simple extraction method utilizing columns pre-packed with diatomaceous earth (Extrelut®) was used. Chromatographic separation was achieved using an isocratic system with a mobile phase of trifluoroacetic acid-water-acetonitrile (0.01:90:10) applied at a flow rate of 1 mL min. -1. Validation studies were also carried out to show that the method is specific, accurate, and precise. In the tested specimens, the lycorine content ranged between 0.008 and 0.364% whereas the galanthamine content was found to be between 0.003 and 0.506%.
Słowa kluczowe
Rocznik
Strony
755--764
Opis fizyczny
Bibliogr. 24 poz., rys., tab.
Twórcy
autor
  • Ege University Department of Pharmacognosy, Faculty of Pharmacy 35100 Bornova, Izmir Turke
  • Ege University Department of Pharmacognosy, Faculty of Pharmacy 35100 Bornova, Izmir Turke
autor
  • Ege University Department of Pharmacognosy, Faculty of Pharmacy 35100 Bornova, Izmir Turke
autor
  • Ege University Department of Pharmacognosy, Faculty of Pharmacy 35100 Bornova, Izmir Turke
autor
  • Ege University Department of Pharmacognosy, Faculty of Pharmacy 35100 Bornova, Izmir Turke
  • Ege University Department of Pharmacognosy, Faculty of Pharmacy 35100 Bornova, Izmir Turke
Bibliografia
  • [1] A.P. Davis, in: M. Bishop, A.P. Davis, and J. Grimshaw (eds) Snowdrops, A Monograph of Cultivated Galanthus, Griffin Press Publishing Ltd., Cheltenham, 2006, pp. 9–63
  • [2] O. Hoshino, in: G.A. Cordell (ed) The Alkaloids Chemistry and Biology, vol. 51, Academic Press Inc., New York, 1998, pp. 323–424
  • [3] N. Unver, Phytochem. Rev., 6, 125 (2007)
  • [4] M. Heinrich, in: G.A. Cordell (ed) The Alkaloids: Chemistry and Biology, vol. 68, Elsevier Inc., San Diego, 2010, pp. 157–165
  • [5] D. Lamoral-Theys, A. Andolfi, G. Van Goietsenoven, A. Cimmino, B. Le Calvé, N. Wauthoz, V. Mégalizzi, T. Gras, C. Bruyére, J. Dubois, V. Mathieu, A. Kornienko, R. Kiss, and A. Evidente, J. Med. Chem., 52, 6244 (2009)
  • [6] S. Li, C. Chen, H. Zhang, H. Guo, H. Wang, L. Wang, X. Zhang, S. Hua, J. Yu, P. Xiao, R. Li, and X. Tan, Antiviral Res., 67, 18 (2005)
  • [7] S. van Dyk, S. Griffiths, R.L. van Zyl, and S.F. Malan, Afr. J. Biotechnol., 8, 5595 (2009)
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  • [9] G.I. Kaya, A. Fillik, Y. Hısıl, and N. Unver, Turkish J. Pharm. Sci., 1, 105 (2004)
  • [10] F.M. Diop, A. Ptak, F. Chretien, M. Henry, Y. Chapleur, and D. Laurain-Mattar, Nat. Prod. Commun., 1, 475 (2006)
  • [11] G.S. Citoglu, B.S. Yılmaz, and O. Bahadır, Chem. Nat. Comp., 44, 826 (2008)
  • [12] G.I. Kaya, D. Cicek, B. Sarıkaya, M.A. Onur, and N. Unver Somer, Nat. Prod. Commun., 5, 873 (2010)
  • [13] S. Berkov, A. Pavlov, M. Ilieva, M. Burrus, S. Popov, and M. Stanilova, Phytochem. Anal., 16, 98 (2005)
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  • [15] S. Berkov, J. Bastida, F. Viladomat, and C. Codina, Phytochem. Anal., 19, 285 (2008)
  • [16] F. Conforti, M.R. Loizzo, M. Marrelli, F. Menichini, G.A. Statti, D. Uzunov, and F. Menichini, Pharm. Biol., 48, 2 (2010)
  • [17] S. Berkov, J. Bastida, F. Viladomat, and C. Codina, Talanta, 83, 1455 (2011)
  • [18] A.H. Abou-Donia, S.M. Toaima, H.M. Hammoda, and E. Shawky, Phytochem. Anal., 19, 353 (2008)
  • [19] T. Mroczek and J. Mazurek, Anal. Chim. Acta, 633, 188 (2009)
  • [20] A. Lubbe, B. Pomahaćová, Y.H. Choi, and R. Verpoorte, Phytochem. Anal., 21, 66 (2010)
  • [21] Latvala, M.A. Onur, T. Gozler, A. Linden, B. Kivcak, and M. Hesse, Phytochemistry, 39, 1229 (1995)
  • [22] ICH Guidelines Q2 (R1), Validation of Analytical Procedures: Text and Methodology, Geneva, November 2005
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  • [24] B. Bozkurt Sarıkaya, N. Unver Somer, and M.A. Onur, Turkish J. Pharm. Sci., 9, 61 (2012)
Typ dokumentu
Bibliografia
Identyfikator YADDA
bwmeta1.element.baztech-11e574b0-6bbb-4008-9d39-17ed300a2194
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