Efficient separation of five flavonoids from Oxytropis falcata bunge by high-speed counter-current chromatography and their anticancer activity

• Autorzy: Chen Chen , Liu Fengqin , Zhao Jingyang , Chen Tao , Li Yulin , Zhang Dejun

Identyfikatory

DOI

10.1556/1326.2019.00672

• Języki publikacji: EN

Abstrakty

EN

Oxytropis falcata Bunge, known as the “king of herbs” in Tibetan medicine, is used for treatment of hyperpyrexia, pain, wounds, inflammation, and anthrax. However, it is difficult to efficiently isolate compounds with high purity from O. falcata because of the complexity of traditional Tibetan medicines. In this study, the 80% ethanol elution fraction from extract by AB-8 macroporous resin column chromatography was demonstrated to have anticancer activity on human hepatoma SMMC-7721 cells in vitro. Then, a high-speed counter-current chromatography (HSCCC) method was successfully established for separation of compounds by using hexane–ethyl acetate–methanol–water (10:4:10:10, v/v/v/v) as the solvent system. Five flavonoids (7-hydroxyflavonone [1], 5,7-dihydroxy-4′-methoxy flavonol [2], 5,7-dihydroxyflavanone [3], 2′,4′-dihydroxychalcone [4], and 2′,4′-dihydroxydihydrochalcone [5]) were obtained in one-step separation with purities of 97.7%, 98.1%, 98.3%, 99.0%, and 98.3%, respectively. Finally, anticancer activities against the growth of SMMC-7721 cells of 5 flavonoids were confirmed. The IC50 values of the separated compounds were 213.45 μg/mL, 197.74 μg/mL, 375.16 μg/mL, 17.44 μg/mL, and 136.83 μg/mL in 24 h, respectively. The present study provided a basis for further development and utilization of this medicinal herb as a source of a new potential anticancer agent.

Słowa kluczowe

EN

Oxytropis falcata Bunge; high speed–counter–current chromatography; flavonoids

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2020
• Tom: Vol. 32, no. 3
• Strony: 189--193
• Opis fizyczny: Bibliogr. 22 poz., rys.

Twórcy

autor

Chen Chen

• Chinese Academy of Sciences, Xining, P. R. China

autor

Liu Fengqin

• Chinese Academy of Sciences, Xining, P. R. China

autor

Zhao Jingyang

• Chinese Academy of Sciences, Xining, P. R. China

autor

Chen Tao

• Chinese Academy of Sciences, Xining, P. R. China

autor

Li Yulin

• Chinese Academy of Sciences, Xining, P. R. China

autor

Zhang Dejun

• Qinghai University, Xining 810016, P. R. China

Bibliografia

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Uwagi

PL

Opracowanie rekordu ze środków MNiSW, umowa Nr 461252 w ramach programu "Społeczna odpowiedzialność nauki" - moduł: Popularyzacja nauki i promocja sportu (2020).