PL
 |
EN

PL EN

Preferencje help
Polish version English version Język
Widoczny [Schowaj] Abstrakt
Liczba wyników
Tytuł artykułu

Easy and affordable method for rapid prototyping of tissue models in vitro using three-dimensional bioprinting

Autorzy
Bandyopadhyay A. , Dewangan V. K. , Vajanthri K. Y. , Poddar S. , Mahto S. K.
Wybrane pełne teksty z tego czasopisma
Identyfikatory
DOI
10.1016/j.bbe.2017.12.001
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
In vitro tissue model systems have attracted considerable attention in drug discovery owing to their ability to facilitate identification of promising compounds in the near-physiological environment during drug development. Additive manufacturing helps in mimicking com-plex geometries including the microarchitecture of the body tissues. Exploiting this emerg-ing technology, the present study demonstrates a simple and inexpensive approach for the fabrication of three-dimensional (3D) in vitro tissue models using a custom-designed automated bioprinting system. The bioink mixture comprised of a novel optimized compo-sition of widely known biomaterials including gelatin, alginate and hydrolyzed type-1 collagen to embed and print the C2C12 myoblast cells. The structural stability and integrity of the cells-laden constructs were found to be significantly consistent for more than 14 days in culture. Rheological and mechanical properties of the bioink blend were characterized to assess its efficacy for the fabrication of cells-laden tissue constructs. Scanning electron micrographs were acquired to analyze porosity of the scaffold for cellular growth and proliferation. The viability of cells embedded within the hydrogel was >80%, 3 h post-printing. We anticipate that the fabricated tissues will serve as an alternative model for in vitro toxicological and drug response studies.
Słowa kluczowe
EN
PL
Wydawca
Nałęcz Institute of Biocybernetics and Biomedical Engineering of the Polish Academy of Sciences
Elsevier
Czasopismo
Biocybernetics and Biomedical Engineering
Rocznik
2018
Tom
Vol. 38, no. 1
Strony
158--169
Opis fizyczny
Bibliogr. 67 poz., rys., wykr.
Twórcy
autor
Bandyopadhyay A.
  • Tissue Engineering and Biomicrofluidics Laboratory, School of Biomedical Engineering, Indian Institute of Technology (Banaras Hindu University), Varanasi 221005, Uttar Pradesh, India
autor
Dewangan V. K.
  • Tissue Engineering and Biomicrofluidics Laboratory, School of Biomedical Engineering, Indian Institute of Technology (Banaras Hindu University), Varanasi 221005, Uttar Pradesh, India
autor
Vajanthri K. Y.
  • Tissue Engineering and Biomicrofluidics Laboratory, School of Biomedical Engineering, Indian Institute of Technology (Banaras Hindu University), Varanasi 221005, Uttar Pradesh, India
autor
Poddar S.
  • Tissue Engineering and Biomicrofluidics Laboratory, School of Biomedical Engineering, Indian Institute of Technology (Banaras Hindu University), Varanasi 221005, Uttar Pradesh, India
autor
Mahto S. K.
skmahto.bme@iitbhu.ac.in
  • Tissue Engineering and Biomicrofluidics Laboratory, School of Biomedical Engineering, Indian Institute of Technology (Banaras Hindu University), Varanasi 221005, Uttar Pradesh, India
Bibliografia
  • [1] Kimlin L, Kassis J, Virador V. 3D in vitro tissue models and their potential for drug screening. Expert Opin Drug Discov 2013;8:1455–66. http://dx.doi.org/10.1517/17460441.2013.852181.
  • [2] Edmondson R, Broglie JJ, Adcock AF, Yang L. Three-dimensional cell culture systems and their applications in drug discovery and cell-based biosensors. Assay Drug Dev Technol 2014;12:207–18. http://dx.doi.org/10.1089/adt.2014.573.
  • [3] Elliott NT, Yuan F. A review of three-dimensional in vitro tissue models for drug discovery and transport studies. J Pharm Sci 2011;100:59–74. http://dx.doi.org/10.1002/jps.22257.
  • [4] Fawcett JW, Barker RA, Dunnett SB. Dopaminergic neuronal survival and the effects of bFGF in explant, three dimensional and monolayer cultures of embryonic rat ventral mesencephalon. Exp Brain Res 1995;106:275–82. http://dx.doi.org/10.1007/BF00241123.
  • [5] Pang Y, Horimoto Y, Sutoko S, Montagne K, Shinohara M, Mathiue D, et al. Novel integrative methodology for engineering large liver tissue equivalents based on threedimensional scaffold fabrication and cellular aggregate assembly. Biofabrication 2016;8:035016. http://dx.doi.org/10.1088/1758-5090/8/3/035016.
  • [6] Lu HF, Leong MF, Lim TC, Chua YP, Lim JK, Du C, et al. Engineering a functional three-dimensional human cardiac tissue model for drug toxicity screening. Biofabrication 2017;9:025011. http://dx.doi.org/10.1088/1758-5090/aa6c3a.
  • [7] Mertens JP, Sugg KB, Lee JD, Larkin LM. Engineering muscle constructs for the creation of functional engineered musculoskeletal tissue. Regen Med 2014;9:89–100. http://dx.doi.org/10.2217/rme.13.81.
  • [8] Knowlton S, Cho Y, Li X-J, Khademhosseini A, Tasoglu S. Utilizing stem cells for three-dimensional neural tissue engineering. Biomater Sci 2016;4:768–84. http://dx.doi.org/10.1039/C5BM00324E.
  • [9] Rönkkö S, Vellonen K-S, Järvinen K, Toropainen E, Urtti A. Human corneal cell culture models for drug toxicity studies. Drug Deliv Transl Res 2016;6:660–75. http://dx.doi.org/10.1007/s13346-016-0330-y.
  • [10] Nichol JW, Khademhosseini A. Modular tissue engineering: engineering biological tissues from the bottom up. Soft Matter 2009;5:1312–9. http://dx.doi.org/10.1039/b814285h.
  • [11] Dean DM, Napolitano AP, Youssef J, Morgan JR. Rods, tori, and honeycombs: the directed self-assembly of microtissues with prescribed microscale geometries. FASEB J Off Publ Fed Am Soc Exp Biol 2007;21:4005–12. http://dx.doi.org/10.1096/fj.07-8710com.
  • [12] Yeh J, Ling Y, Karp JM, Gantz J, Chandawarkar A, Eng G, et al. Micromolding of shape-controlled, harvestable cell-laden hydrogels. Biomaterials 2006;27:5391–8. http://dx.doi.org/10.1016/j.biomaterials.2006.06.005.
  • [13] Bettadapur A, Suh GC, Geisse NA, Wang ER, Hua C, Huber HA, et al. Prolonged culture of aligned skeletal myotubes on micromolded gelatin hydrogels. Sci Rep 2016;6:28855. http://dx.doi.org/10.1038/srep28855.
  • [14] L'Heureux N, Pâquet S, Labbé R, Germain L, Auger FA. A completely biological tissue-engineered human blood vessel. FASEB J Off Publ Fed Am Soc Exp Biol 1998;12:47–56.
  • [15] Mironov V, Boland T, Trusk T, Forgacs G, Markwald RR. Organ printing: computer-aided jet-based 3D tissue engineering. Trends Biotechnol 2003;21:157–61. http://dx.doi.org/10.1016/S0167-7799(03)00033-7.
  • [16] Cittadella Vigodarzere G, Mantero S. Skeletal muscle tissue engineering: strategies for volumetric constructs. Front Physiol 2014;5:1–13. http://dx.doi.org/10.3389/fphys.2014.00362.
  • [17] Madden L, Juhas M, Kraus WE, Truskey GA, Bursac N. Bioengineered human myobundles mimic clinical responses of skeletal muscle to drugs. ELife 2015;4:e04885. http://dx.doi.org/10.7554/eLife.04885.
  • [18] Ozbolat IT, Hospodiuk M. Current advances and future perspectives in extrusion-based bioprinting. Biomaterials 2016;76:321–43. http://dx.doi.org/10.1016/j.biomaterials.2015.10.076.
  • [19] Chua CK, Yeong WY. Bioprinting: principles and applications. World Scientific Publishing Co Inc.; 2014.
  • [20] Bartolo PJ, da S. Innovative developments in virtual and physical prototyping. Proceedings of the 5th International Conference on Advanced Research in Virtual and Rapid Prototyping. CRC Press; 2011.
  • [21] Ozbolat IT, Yu Y. Bioprinting toward organ fabrication: challenges and future trends. IEEE Trans Biomed Eng 2013;60:691–9. http://dx.doi.org/10.1109/TBME.2013.2243912.
  • [22] Murphy SV, Atala A. 3D bioprinting of tissues and organs. Nat Biotechnol 2014;32:773–85. http://dx.doi.org/10.1038/nbt.2958.
  • [23] Atala A. Tissue engineering and regenerative medicine: concepts for clinical application. Rejuvenation Res 2004;7:15–31. http://dx.doi.org/10.1089/154916804323105053.
  • [24] Choi Y-J, Kim TG, Jeong J, Yi H-G, Park JW, Hwang W, et al. 3D cell printing of functional skeletal muscle constructs using skeletal muscle-derived bioink. Adv Healthc Mater 2016;5:2636–45. http://dx.doi.org/10.1002/adhm.201600483.
  • [25] Kang H-W, Lee SJ, Ko IK, Kengla C, Yoo JJ, Atala A. A 3D bioprinting system to produce human-scale tissue constructs with structural integrity. Nat Biotechnol 2016;34:312–9. http://dx.doi.org/10.1038/nbt.3413.
  • [26] Cui X, Gao G, Qiu Y. Accelerated myotube formation using bioprinting technology for biosensor applications. Biotechnol Lett 2013;35:315–21. http://dx.doi.org/10.1007/s10529-012-1087-0.
  • [27] Yeatts AB, Fisher JP. Tubular perfusion system for the longterm dynamic culture of human mesenchymal stem cells. Tissue Eng Part C Methods 2010;17:337–48. http://dx.doi.org/10.1089/ten.tec.2010.0172.
  • [28] Mehrban N, Teoh GZ, Birchall MA. 3D bioprinting for tissue engineering: stem cells in hydrogels. Int J Bioprinting 2016;2:6–19. http://dx.doi.org/10.18063/IJB.2016.01.006.
  • [29] Dababneh AB, Ozbolat IT. Bioprinting technology: a current state-of-the-art review. J Manuf Sci Eng 2014;136. http://dx.doi.org/10.1115/1.4028512. 061016-061016-11.
  • [30] Lee W, Debasitis JC, Lee VK, Lee J-H, Fischer K, Edminster K, et al. Multi-layered culture of human skin fibroblasts and keratinocytes through three-dimensional freeform fabrication. Biomaterials 2009;30:1587–95. http://dx.doi.org/10.1016/j.biomaterials.2008.12.009.
  • [31] Lee V, Singh G, Trasatti JP, Bjornsson C, Xu X, Tran TN, et al. Design and fabrication of human skin by three-dimensional bioprinting. Tissue Eng Part C Methods 2013;20:473–84. http://dx.doi.org/10.1089/ten.tec.2013.0335.
  • [32] Ng WL, Yeong WY, Naing MW. Polyelectrolyte gelatin-chitosan hydrogel optimized for 3D bioprinting in skin tissue engineering. Int J Bioprinting 2016;2:53–62.
  • [33] Smith CM, Stone AL, Parkhill RL, Stewart RL, Simpkins MW, Kachurin AM, et al. Three-dimensional bioassembly tool for generating viable tissue-engineered constructs. Tissue Eng 2004;10:1566–76. http://dx.doi.org/10.1089/ten.2004.10.1566.
  • [34] Markstedt K, Mantas A, Tournier I, Martínez Ávila H, Hägg D, Gatenholm P. 3D bioprinting human chondrocytes with nanocellulose–alginate bioink for cartilage tissue engineering applications. Biomacromolecules 2015;16:1489–96. http://dx.doi.org/10.1021/acs.biomac.5b00188.
  • [35] Visser J, Peters B, Burger TJ, Boomstra J, Dhert WJA, Melchels FPW, et al. Biofabrication of multi-material anatomically shaped tissue constructs. Biofabrication 2013;5:035007. http://dx.doi.org/10.1088/1758-5082/5/3/035007.
  • [36] Shim J-H, Lee J-S, Kim JY, Cho D-W. Bioprinting of a mechanically enhanced three-dimensional dual cell-laden construct for osteochondral tissue engineering using a multi-head tissue/organ building system. J Micromech Microeng 2012;22:085014. http://dx.doi.org/10.1088/0960-1317/22/8/085014.
  • [37] Chameettachal S, Midha S, Ghosh S. Regulation of chondrogenesis and hypertrophy in silk fibroin-gelatin-based 3D bioprinted constructs. ACS Biomater Sci Eng 2016;2:1450–63. http://dx.doi.org/10.1021/acsbiomaterials.6b00152.
  • [38] Pati F, Jang J, Ha D-H, Kim SW, Rhie J-W, Shim J-H, et al. Printing three-dimensional tissue analogues with decellularized extracellular matrix bioink. Nat Commun 2014;5. http://dx.doi.org/10.1038/ncomms4935. Ncomms4935.
  • [39] Gaetani R, Doevendans PA, Metz CHG, Alblas J, Messina E, Giacomello A, et al. Cardiac tissue engineering using tissue printing technology and human cardiac progenitor cells. Biomaterials 2012;33:1782–90. http://dx.doi.org/10.1016/j.biomaterials.2011.11.003.
  • [40] Costantini M, Testa S, Mozetic P, Barbetta A, Fuoco C, Fornetti E, et al. Microfluidic-enhanced 3D bioprinting of aligned myoblast-laden hydrogels leads to functionally organized myofibers in vitro and in vivo. Biomaterials 2017;131:98–110. http://dx.doi.org/10.1016/j.biomaterials.2017.03.026.
  • [41] Mozetic P, Giannitelli SM, Gori M, Trombetta M, Rainer A. Engineering muscle cell alignment through 3D bioprinting. J Biomed Mater Res A 2017;105:2582–8. http://dx.doi.org/10.1002/jbm.a.36117.
  • [42] Kizawa H, Nagao E, Shimamura M, Zhang G, Torii H. Scaffold-free 3D bio-printed human liver tissue stably maintains metabolic functions useful for drug discovery. Biochem Biophys Rep 2017;10:186–91. http://dx.doi.org/10.1016/j.bbrep.2017.04.004.
  • [43] Bertassoni LE, Cardoso JC, Manoharan V, Cristino AL, Bhise NS, Araujo WA, et al. Direct-write bioprinting of cell-laden methacrylated gelatin hydrogels. Biofabrication 2014;6:024105. http://dx.doi.org/10.1088/1758-5082/6/2/024105.
  • [44] Cohen DL, Lipton JI, Bonassar LJ, Lipson H. Additive manufacturing for in situ repair of osteochondral defects. Biofabrication 2010;2:035004. http://dx.doi.org/10.1088/1758-5082/2/3/035004.
  • [45] Seyednejad H, Gawlitta D, Dhert WJA, van Nostrum CF, Vermonden T, Hennink WE. Preparation and characterization of a three-dimensional printed scaffold based on a functionalized polyester for bone tissue engineering applications. Acta Biomater 2011;7:1999–2006. http://dx.doi.org/10.1016/j.actbio.2011.01.018.
  • [46] Fedorovich NE, De Wijn JR, Verbout AJ, Alblas J, Dhert WJA. Three-dimensional fiber deposition of cell-laden, viable, patterned constructs for bone tissue printing. Tissue Eng Part A 2008;14:127–33. http://dx.doi.org/10.1089/ten.a.2007.0158.
  • [47] Chung JHY, Naficy S, Yue Z, Kapsa R, Quigley A, Moulton SE, et al. Bio-ink properties and printability for extrusion printing living cells. Biomater Sci 2013;1:763–73. http://dx.doi.org/10.1039/C3BM00012E.
  • [48] Burattini S, Ferri P, Battistelli M, Curci R, Luchetti F, Falcieri E. C2C12 murine myoblasts as a model of skeletal muscle development: morpho-functional characterization. Eur J Histochem 2004;48:223–33.
  • [49] Semsarian C, Wu M-J, Ju Y-K, Marciniec T, Yeoh T, Allen DG, et al. Skeletal muscle hypertrophy is mediated by a Ca2+- dependent calcineurin signalling pathway. Nature 1999;400:23054. http://dx.doi.org/10.1038/23054.
  • [50] Garcia LA, King KK, Ferrini MG, Norris KC, Artaza JN. 1,25 (OH)2vitamin D3 stimulates myogenic differentiation by inhibiting cell proliferation and modulating the expression of promyogenic growth factors and myostatin in C2C12 skeletal muscle cells. Endocrinology 2011;152:2976–86. http://dx.doi.org/10.1210/en.2011-0159.
  • [51] Bains W, Ponte P, Blau H, Kedes L. Cardiac actin is the major actin gene product in skeletal muscle cell differentiation in vitro. Mol Cell Biol 1984;4:1449–53. http://dx.doi.org/10.1128/MCB.4.8.1449.
  • [52] Bodine SC, Latres E, Baumhueter S, Lai VK-M, Nunez L, Clarke BA, et al. Identification of ubiquitin ligases required for skeletal muscle atrophy. Science 2001;294:1704–8. http://dx.doi.org/10.1126/science.1065874.
  • [53] Sharples AP, Player DJ, Martin NRW, Mudera V, Stewart CE, Lewis MP. Modelling in vivo skeletal muscle ageing in vitro using three-dimensional bioengineered constructs. Aging Cell 2012;11:986–95. http://dx.doi.org/10.1111/j.1474-9726.2012.00869.x.
  • [54] Huang NF, Lee RJ, Li S. Engineering of aligned skeletal muscle by micropatterning. Am J Transl Res 2010;2:43–55.
  • [55] Bajaj P, Reddy B, Millet L, Wei C, Zorlutuna P, Bao G, et al. Patterning the differentiation of C2C12 skeletal myoblasts. Integr Biol 2011;3:897–909. http://dx.doi.org/10.1039/C1IB00058F.
  • [56] Jun I, Jeong S, Shin H. The stimulation of myoblast differentiation by electrically conductive sub-micron fibers. Biomaterials 2009;30:2038–47. http://dx.doi.org/10.1016/j.biomaterials.2008.12.063.
  • [57] Agrawal G, Aung A, Varghese S. Skeletal muscle-on-a-chip: an in vitro model to evaluate tissue formation and injury. Lab Chip 2017. http://dx.doi.org/10.1039/C7LC00512A.
  • [58] Duan B, Hockaday LA, Kang KH, Butcher JT. 3D bioprinting of heterogeneous aortic valve conduits with alginate/ gelatin hydrogels. J Biomed Mater Res A 2013;101A:1255–64. http://dx.doi.org/10.1002/jbm.a.34420.
  • [59] Zhao Y, Yao R, Ouyang L, Ding H, Zhang T, Zhang K, et al. Three-dimensional printing of Hela cells for cervical tumor model in vitro. Biofabrication 2014;6:035001. http://dx.doi.org/10.1088/1758-5082/6/3/035001.
  • [60] Goldstein TA, Epstein CJ, Schwartz J, Krush A, Lagalante DJ, Mercadante KP, et al. Feasibility of bioprinting with a modified desktop 3D printer. Tissue Eng Part C Methods 2016;22:1071–6. http://dx.doi.org/10.1089/ten.tec.2016.0286.
  • [61] Grzesik WJ, Robey PG. Bone matrix RGD glycoproteins: immunolocalization and interaction with human primary osteoblastic bone cells in vitro. J Bone Miner Res Off J Am Soc Bone Miner Res 1994;9:487–96. http://dx.doi.org/10.1002/jbmr.5650090408.
  • [62] Panwar A, Tan LP. Current status of bioinks for micro- extrusion-based 3D bioprinting. Molecules 2016;21:685. http://dx.doi.org/10.3390/molecules21060685.
  • [63] Bagley JR, Galpin AJ. Three-dimensional printing of human skeletal muscle cells: an interdisciplinary approach for studying biological systems. Biochem Mol Biol Educ Bimon Publ Int Union Biochem Mol Biol 2015;43:403–7. http://dx.doi.org/10.1002/bmb.20891.
  • [64] Linnes MP, Ratner BD, Giachelli CM. A fibrinogen-based precision microporous scaffold for tissue engineering. Biomaterials 2007;28:5298–306. http://dx.doi.org/10.1016/j.biomaterials.2007.08.020.
  • [65] Lévesque SG, Lim RM, Shoichet MS. Macroporous interconnected dextran scaffolds of controlled porosity for tissue-engineering applications. Biomaterials 2005;26:7436–46. http://dx.doi.org/10.1016/j.biomaterials.2005.05.054.
  • [66] Van Loocke M, Lyons CG, Simms CK. A validated model of passive muscle in compression. J Biomech 2006;39: 2999–3009. http://dx.doi.org/10.1016/j.jbiomech.2005.10.016.
  • [67] Van Sligtenhorst C, Cronin DS, Wayne Brodland G. High strain rate compressive properties of bovine muscle tissue determined using a split Hopkinson bar apparatus. J Biomech 2006;39:1852–8. http://dx.doi.org/10.1016/j.jbiomech.2005.05.015.
Uwagi
PL
Opracowanie rekordu w ramach umowy 509/P-DUN/2018 ze środków MNiSW przeznaczonych na działalność upowszechniającą naukę (2018).
Typ dokumentu
Bibliografia
Identyfikator YADDA
bwmeta1.element.baztech-0dfec829-8260-4950-82d5-b9f33c1d113e
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.