Identification of Oil Degrading Bacteria from Oil-Contaminated Soil in the Northeastern Part of Jordan

• Autorzy: Aladwan Mohammad Mahmoud , Dababneh Basem Fou'ad , Farah Husni Shukri , Abusalah Mai Abdel Haleem

Identyfikatory

DOI

10.12911/22998993/186502

• Języki publikacji: EN

Abstrakty

EN

Bioremediation aspects of crude oil-polluted fields can be achieved by isolating and identifying bacterial species from oil-contaminated soil. This allows for the selection of the most active isolates and the enhancement of the effectiveness of other bacteria. This project will be a base to use green technology for clean the oil contaminated soil in Jordan. This study involved the isolation and identification of oil-degrading microbes from soil samples contaminated with oil in the northeastern region of Jordan. The morphological and biochemical tests were used to characterize twenty-five bacterial isolates. Molecular identification of a universal primer 16S rDNA gene was used to identify bacterial isolates. Total petroleum hydrocarbons were analyzed using gas chromatography for soil samples. All soil samples were analyzed for heavy metal contamination (Cu, Cd, Mn, Zn, and Pb). The bacterial growth count (CFU/g) was between 1.06×10⁵ and 2.80×10¹⁷. The identified bacterial genera included: Staphylococcus, Citrobacter, Lactobacillus, Alcaligin’s, Pseudomonas, Micrococcus, Serratia, Enterobacter, Bacillus, Salmonella, Mycobacterium, Corynebacterium, and, Microbacterium. The most species showed high growth rates on different types of hydrocarbons such as toluene, naphthalene, and hexane were Lactobacillus casei, Staphylococcus intermedius, Micrococcus luteus, Pseudomonas putida, Mycobacterium phlei, Corynebacterium xerosis. Soil sample M1A contains the highest levels of Fe, Cd , and Pb and Cu,. While M1C contains the highest levels of Fe and Mn. On the other hand, M2A, and M2C have the least levels of Mn and Fe. While M3C has the least level of Zn and Pb. our study conclude the bacterial isolates could be used for in situ and ex situ cleanup of oil-contaminated desert soil in northeastern part of Jordan.

Słowa kluczowe

EN

hydrocarbons; bioremediation; 16S rDNA gene; oil degrading bacteria

• Wydawca: Polskie Towarzystwo Inżynierii Ekologicznej ; Lublin University of Technology
• Czasopismo: Journal of Ecological Engineering
• Rocznik: 2024
• Tom: Vol. 25, nr 5
• Strony: 306--320
• Opis fizyczny: Bibliogr. 37 poz., rys., tab.

Twórcy

autor

Aladwan Mohammad Mahmoud

• Al-Ahliyya Amman University, Amman 19111, Jordan

• https://orcid.org/0000-0002-4797-836X

autor

Dababneh Basem Fou'ad

• Al-Ahliyya Amman University, Amman 19111, Jordan

autor

Farah Husni Shukri

• Al-Ahliyya Amman University, Amman 19111, Jordan

autor

Abusalah Mai Abdel Haleem

• Al-Ahliyya Amman University, Amman 19111, Jordan

• https://orcid.org/0000-0002-8473-9392

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