Triterpenes in traditional and supercritical-fluid extracts of Morus alba leaf and stem bark

• Autorzy: Böszörményi A , Szarka Sz. , Héthelyi É , Gyurján I , László M , Simándi B , Szőke É , Lemberkovics É
• Języki publikacji: EN

Abstrakty

EN

Our objectives were to establish a GC method capable of quantitative analysis of terpenoids without derivatisation and to examine the amount of β -sitosterol extracted from Morus alba L. leaf and stem bark by use of traditional organic solvent extraction and supercritical-fluid extraction (SFE). To measure β -sitosterol content without derivatization, GC-FID was used with 5- α -cholestan-3-one as internal standard. To identify terpenoid constituents, GC-MS was used; β -sitosterol, phytol, lanost-7-en-3-on, α -amyrin, β -amyrin, and lupeol were identified. We established that for Morus leaf the best SFE method for β -sitosterol was pilot scale SFE; the β -sitosterol content of this extract was higher than that of the hexane solvent extract. Among analytical SFE conditions, 200 bar for 90 min and 300 bar for 60 min resulted in extraction of the most β -sitosterol. For mulberry stem bark, solvent extraction with hexane and SFE at 400 bar and 40°C for 60 min proved the best methods.

Słowa kluczowe

EN

Morus alba L; mulberry leaf; stem bark; supercritical fluid extraction (SFE); gas chromatography-mass spectrometry; β-sitosterol; α and β-amyrin; lupeol; lanost-7-en-3-one

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2009
• Tom: Vol. 21, no. 4
• Strony: 659--669
• Opis fizyczny: Bibliogr. 25 poz., rys., tab.

Twórcy

autor

Böszörményi A

• Semmelweis University Department of Pharmacognosy Üllői út 26 1085 Budapest Hungary

autor

Szarka Sz.

• Semmelweis University Department of Pharmacognosy Üllői út 26 1085 Budapest Hungary

autor

Héthelyi É

• Eötvös Loránd University Department of Plant Anatomy Pázmány Péter út 1/C 1117 Budapest Hungary

autor

Gyurján I

• Budapest University of Technology and Economics Department of Chemical and Environmental Process Engineering Műegyetem rkp. 3. 1111 Budapest Hungary

autor

László M

• Eötvös Loránd University Department of Plant Anatomy Pázmány Péter út 1/C 1117 Budapest Hungary

autor

Simándi B

• Budapest University of Technology and Economics Department of Chemical and Environmental Process Engineering Műegyetem rkp. 3. 1111 Budapest Hungary

autor

Szőke É

• Semmelweis University Department of Pharmacognosy Üllői út 26 1085 Budapest Hungary

autor

Lemberkovics É

• Semmelweis University Department of Pharmacognosy Üllői út 26 1085 Budapest Hungary

Bibliografia

• [1] N. Asano, T. Yamashita, K. Yasuda, K. Ikeda, H. Kizu, and Y. Kameda, J. Agric. Food. Chem., 4 , 4208 (2001)
• [2] K. Szendrei, K. Csedő, and A. Hunyadi, Gyógyszerészet, 50 , 243 (2006)
• [3] C. Miyahara, M. Miyazawa, S. Satoh, A. Sakai, and S. Mizusaki, J. Nutr. Sci. Vitaminol., 50 , 161 (2004)
• [4] C. Hansawasdi and J. Kawabata, Fitoterapia, 77 , 568 (2006)
• [5] N. Asano, K. Oseki, E. Tomioka, H. Kizu, and K. Matsui, Carbohydr. Res., 259 , 243 (1994)
• [6] J.M. Rollinger, A. Bodensieck, C. Seger, E.P. Ellmerer, R. Bauer, T. Langer, and H. Stuppner, Planta Med., 71 , 399 (2005)
• [7] T. Katsube, N. Imawaka, Y. Kawano, Y. Yamazaki, K. Shiwaku, and Y. Yamane, Food Chem., 97 , 25 (2006)
• [8] S.Y. Kim, J.J. Gao, and W.C. Lee, Arch. Pharm. Res., 22 , 81 (1999)
• [9] R.R. Berges, J. Windeler, H.J. Trampisch, and T.H. Senge, Lancet, 3451 , 592 (1995)
• [10] A.B. Awad, M.S. Hartati, and C.S. Fink, J. Nutr. Biochem., 9 , 712 (1998)
• [11] D. Li, M. Dong, W.J. Shim, and N. Kannan, J. Chromatogr. A, 1160 , 64 (2007)
• [12] N. Souchet and S. Lamplante, Biochem. Mol. Biol., 147 , 378 (2007)
• [13] M. Hasmeda, G. Kweifio-Okai, T. Macrides, and G.M. Polya, Planta Med., 65 , 14 (1999)
• [14] P.H. List and L. Hörhammer, Hagers Handbuch der Pharmazeutischen Praxis, Vol. 5, Springer, Berlin, 1976, pp. 899–900
• [15] T. Okuzumi, Y. Kaji, H. Hamada, and Y. Fujimoto, Tetrahedron Lett., 41 , 3623 (2000)
• [16] D.D. Kulkarni, Indian J. Exp. Biol., 8 , 347 (1970)
• [17] C. Domeno, B. Ruiz, and C. Nerín, Anal. Bioanal. Chem., 381 , 1576 (2005)
• [18] A.K. Batta, G. Salen, P. Batta, G.S. Tint, and D.S. Alberts, J. Chromatogr. B, 75 , 153 (2002)
• [19] A. Gutierrez, J.C. del Rio, F.J. Gonzalez-Vila, and F. Martin, J. Chromatogr. A, 823 , 449 (1998)
• [20] European Pharmacopoeia, Supplement 5.0, EDQM, Strasbourg, France, 2005, p. 222
• [21] T. Allen, Particle Size Measurement, Chapman and Hall, London, 1981
• [22] B. Nagy, B. Simándi, and Cs.D. Andras, J. Food Eng., 88 , 104 (2008)
• [23] E. Rónyai, B. Simándi, A. Kéry, E. Lemberkovics, M. Then, and A. Csordás, Olaj, Szappan, Kozmetika, 45 , 94 (1996)
• [24] M. Oszagyán, B. Simándi, J. Sawinsky, A. Kéry, E. Lemberkovics, and J. Fekete, Flavour Fragr. J. 11 , 157 (1996)
• [25] European Pharmacopoeia Supplement 5.0, EDQM, Strasbourg, France, 2005, p. 129