Pharmacokinetics and bioavailability of liensinine in mouse blood by UPLC-MS/MS

• Autorzy: Tong Shuhua , Zeng Yuqi , Ma Jianshe , Wen Congcong

Identyfikatory

DOI

10.1556/1326.2020.00847

• Języki publikacji: EN

Abstrakty

EN

Liensinine is a bisbenzyltetrahydroisoquinoline alkaloid extracted from lotus (Nelumbo nucifera GAERTNER., Nelumbonaceae), especially in its embryo loti “Lien Tze Hsin” (green embryo of mature seed). A rapid and simple UPLC-MS/MS method was developed to determine liensinine in mouse blood and its application to a pharmacokinetic study. The blood samples were preprocessed by protein precipitation using acetonitrile. Midazolam (internal standard, IS) and liensinine were gradient eluted by mobile phase of methanol and water (0.1% formic acid) in a Waters UPLC BEH C18 column. The multiple reaction monitoring of m/z 611.3 → 206.1 for liensinine and m/z 326.2 → 291.1 for IS with an electrospray ionization (ESI) source was used for quantitative detection. The calibration curve ranged from 0.5 to 400 ng/mL (r > 0.995). The accuracy ranged from 92.2 to 108.2%, the precision of intra-day and inter-day was less than 14%, and the matrix effect was between 100.0% and 109.6%, the recovery was better than 71.0%. The developed UPLC-MS/MS method was successfully used for a pharmacokinetic study of liensinine in mice after oral (5 mg/kg) and intravenous administration (1 mg/kg), and the absolute availability of liensinine was 1.8%.

Słowa kluczowe

EN

liensinine; mice; UPLC-MS/MS; determination; bioavailability

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2021
• Tom: Vol. 33, no. 4
• Strony: 333--337
• Opis fizyczny: Bibliogr. 15 poz., rys., tab.

Twórcy

autor

Tong Shuhua

• Department of Pharmacy, Maternal and Child Health Hospital of Hubei Province, Wuhan, China

autor

Zeng Yuqi

• Laboratory Animal Centre, Wenzhou Medical University, Wenzhou, China

autor

Ma Jianshe

• Laboratory Animal Centre, Wenzhou Medical University, Wenzhou, China

autor

Wen Congcong

• Laboratory Animal Centre, Wenzhou Medical University, Wenzhou, China

• https://orcid.org/0000-0001-8413-1697

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