Simultaneously measure the concentrations of busulfan and phenytoin in human plasma using an HPLC-MS/MS method: Application to the TDM for children underwent hematological stem cell transplantation

• Autorzy: Sun Ning , Li Zhuo , Zhang Meng , He Huan , Zhao Libo , Mei Dong , Zhu Guanghua , Wang Xiaoling

Identyfikatory

DOI

10.1556/1326.2022.01069

• Języki publikacji: EN

Abstrakty

EN

In this work, a simple and rapid high performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS) method was developed and validated to carry out the simultaneous measurement of busulfan (BU) and phenytoin (PHT) in the plasma of children. In this method, plasma sample could be prepared by one-step protein precipitation using 1 mL of methanol/water (1:1, v/v). After centrifugation (14,500 rpm, 5 min, 4 °C), 10 μL of the supernatant was injected into a Hypersil Gold C18 column (150 × 2.1 mm, 5 μm, Thermo Fisher Scientific) for separation by gradient elution. Quantification was carried out using multiple reactions monitoring (MRM) under positive scan mode. In the method verification, the calibration curves of BU and PHT showed satisfactory linearity (r > 0.99) at the concentration ranging from 0.02 to 20 μg mL⁻¹. The accuracy and precision were tested at four concentration levels (including the LLOQ level) with the relative error (RE) ranging from −0.80% to 11.45% and coefficient of variation (CV) between 0.93% and 7.74%. There was no pronounced matrix effect to interfere with the quantitative analysis. Compared to determine BU and PHT using two individual methods, less pre-treatment process, labor and blood sample volume are required in this proposed method. Finally, this method was successfully applied to the therapeutic drug monitoring of BU and PHT for children underwent hematological stem cell transplantation.

Słowa kluczowe

EN

busulfan; phenytoin; HPLC-MS/MS; therapeutic drug monitoring

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2023
• Tom: Vol. 35, no. 4
• Strony: 302--309
• Opis fizyczny: Bibliogr. 17 poz., tab., wykr.

Twórcy

autor

Sun Ning

• Pharmaceutical Department, Beijing Children’s Hospital, Capital Medical University, National Center for Children’s Health, Beijing 100045, China

• https://orcid.org/0000-0001-6142-311X

autor

Li Zhuo

• Pharmaceutical Department, Beijing Children’s Hospital, Capital Medical University, National Center for Children’s Health, Beijing 100045, China
• School of Basic Medicine and Clinical Pharmacy, China Pharmaceutical University, Nanjing 210009, China

• https://orcid.org/0000-0003-4596-042X

autor

Zhang Meng

• Pharmaceutical Department, Beijing Children’s Hospital, Capital Medical University, National Center for Children’s Health, Beijing 100045, China

autor

He Huan

• Pharmaceutical Department, Beijing Children’s Hospital, Capital Medical University, National Center for Children’s Health, Beijing 100045, China

autor

Zhao Libo

• Pharmaceutical Department, Beijing Children’s Hospital, Capital Medical University, National Center for Children’s Health, Beijing 100045, China

autor

Mei Dong

• Pharmaceutical Department, Beijing Children’s Hospital, Capital Medical University, National Center for Children’s Health, Beijing 100045, China

autor

Zhu Guanghua

• Hematopoietic Stem Cell Transplantation Department, Beijing Children’s Hospital, Capital Medical University, National Center for Children’s Health, Beijing 100045, China

• https://orcid.org/0000-0002-3684-0613

autor

Wang Xiaoling

• Pharmaceutical Department, Beijing Children’s Hospital, Capital Medical University, National Center for Children’s Health, Beijing 100045, China

• https://orcid.org/0000-0003-2136-7410

Bibliografia

• 1. Hoy, S.M.; Lyseng-Williamson, K.A. Paediatr. Drugs 2007, 9, 271–8.
• 2. Andersson, B.S.; Thall, P.F.; Madden, T.; Couriel, D.; Wang, X.; Tran, H.T.; Anderlini, P.; De Lima, M.; Gajewski, J.; Champlin, R.E. Biol. Blood Marrow Transpl. 2002, 8, 477–85.
• 3. Nath, C.E.; Shaw, P.J. Curr. Clin. Pharmacol. 2007, 2, 75–91.
• 4. Deeg, H.J.; Maris, M.B.; Scott, B.L.; Warren, E.H. Leukemia 2006, 20, 1701–5.
• 5. Eberly, A.L.; Anderson, G.D.; Bubalo, J.S.; McCune, J.S. Pharmacotherapy 2008, 28, 1502–10.
• 6. Grigg, A.P.; Shepherd, J.D.; Phillips, G.L. Ann. Intern. Med. 1989, 111, 1049–50.
• 7. Akiyama, K.; Kume, T.; Fukaya, M.; Shiki, I.; Enami, T.; Tatara, R.; Shino, M.; Ikeda, T. Cancer Chemother. Pharmacol. 2018, 82, 717–21.
• 8. Datta, P.; Scurlock, D.; Dasgupta, A. Ther. Drug Monit. 2005, 27, 305–8.
• 9. Courtney, J.B.; Harney, R.; Li, Y.; Lundell, G.; McMillin, G.A.; Agarwal, G.; Salamone, S. J. Ther. Drug Monit. 2009, 31, 489–94.
• 10. Quernin, M.; Poonkuzhali, B.; Médard, Y.; Dennison, D.; Srivastava, A.; Krishnamoorthy, R.; Chandy, M.; Jacqz-Aigrain, E. J. Chromatogr. B 1999, 721, 147–52.
• 11. Xiao, Y.; Li, X.; Fu, X. Clinica Chim. Acta 2018, 479, 190–5.
• 12. Hösli, R.; Tobler, A.; König, S.; Mühlebach, S. J. Anal. Toxicol. 2013, 37, 102–9.
• 13. Versace, F.; Uppugunduri, C.R.; Krajinovic, M.; Théorêt, Y.; Gumy-Pause, F.; Mangin, P.; Staub, C.; Ansari, M. Anal. Bioanal. Chem. 2012, 404, 1831–8.
• 14. Hösli, R.; König, S.; Mühlebach, S.F. J. Anal. Methods Chem. 2018, 8274131.
• 15. French, D.; Sujishi, K.K.; Long-Boyle, J.R.; Ritchie, J.C. Ther. Drug Monit. 2014, 36, 169–74.
• 16. Yuan, J.; Sun, N.; Zhang, S.; Feng, X.; Chen, X.; Zhao, D.; Zhao, L. Biomed. Chromatogr. 2020, e4898.
• 17. Pebdania, A.A.; Dadfarniaa, S.; Shabania, A.M.; Khodadoustb, S.; Talebianpoorc, M.S. J. Separat. Sci. 2017, 41, 509–17.