Application of gamma radiation and physicochemical treatment to improve the bioactive properties of chitosan extracted from shrimp shell

• Autorzy: Aktar J. , Hasan Z. , Afroz T. , or-Rashid H. , Pramanik K.

Identyfikatory

DOI

10.1515/nuka-2017-0036

• Konferencja: International Conference on Applications of Radiation Science and Technology (ICARST-2017) (24-28 April 2017 ; Vienna, Austria)
• Języki publikacji: EN

Abstrakty

EN

The aim of this study is to exploit a suitable chitosan extraction method from the chitin of indigenous shrimp shells by employing different physicochemical treatments and to improve different bioactive properties of this extracted chitosan (CS) by applying gamma radiation. Chitin was prepared from shrimp shell by pretreatment (deproteination, demineralization and oxidation). Chitosan was extracted from chitin by eight different methods varying different physicochemical parameters (reagent concentration, temperature and time) and assessed with respect to the degree of deacetylation, requirement of time and reagents. The method where chitin was repeatedly treated with 121°C for 30 min with 20 M NaOH, produced the highest degree of deacetylation (DD) value (92%) as measured by potentiometric titration, with the least consumption of time and chemicals, and thus, selected as the best suitable extraction method. For further quality improvement, chitosan with highest DD value was irradiated with different doses (i.e., 5, 10, 15, 20 and 50 kGy) of gamma radiation from cobalt-60 gamma irradiator. As the radiation dose was increased, the molecular weight of the wet irradiated chitosan, as measured by the viscosimetric method, decreased from 1.16 × 105 to 1.786 × 103 , 1.518 × 103 , 1.134 × 103 , 1.046 × 103 and 8.23 × 102 dalton, respectively. The radiation treatment of chitosan samples increased the antimicrobial activity signifi cantly in concentration dependent manner on both gram-positive (Staphylococcus aureus) and gram-negative (Escherichia coli) bacteria, as determined by the well-diffusion method. Four to fi ve percent wet chitosan treated with a radiation dose range of 5.0–10.0 kGy rendered the highest antimicrobial activity with least energy and time consumption. Solubility, water binding capacity (WBC) and fat binding capacity (FBC) also improved due to irradiation of chitosan.

Słowa kluczowe

EN

chitosan; gamma radiation; degree of deacetylation; antimicrobial activity

• Wydawca: Institute of Nuclear Chemistry and Technology
• Czasopismo: Nukleonika
• Rocznik: 2017
• Tom: Vol. 62, No. 4
• Strony: 245--251
• Opis fizyczny: Bibliogr. 16 poz., rys.

Twórcy

autor

Aktar J.

• Department of Zoology, Faculty of Biological Science, Jahangirnagar University, Savar, Dhaka, Bangladesh

autor

Hasan Z.

• Microbiology and Industrial Irradiation Division, Institute of Food and Radiation Biology, Atomic Energy Research Establishment, Ganakbari, Savar, Dhaka, Bangladesh, Tel.: [phone]

autor

Afroz T.

• Department of Zoology, Faculty of Biological Science, Jahangirnagar University, Savar, Dhaka, Bangladesh

autor

or-Rashid H.

• Microbiology and Industrial Irradiation Division, Institute of Food and Radiation Biology, Atomic Energy Research Establishment, Ganakbari, Savar, Dhaka, Bangladesh, Tel.: [phone]

autor

Pramanik K.

• Microbiology and Industrial Irradiation Division, Institute of Food and Radiation Biology, Atomic Energy Research Establishment, Ganakbari, Savar, Dhaka, Bangladesh, Tel.: [phone]

Bibliografia

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Uwagi

PL

Opracowanie ze środków MNiSW w ramach umowy 812/P-DUN/2016 na działalność upowszechniającą naukę (zadania 2017).