Comparison of qNANO results from the isolation of extracellular microvesicles with the theoretical model

• Autorzy: Durak-Kozica Martyna , Wróbel Andrzej , Platt Mark , Stępień Ewa Ł.

Identyfikatory

DOI

10.2478/bioal-2022-0088

• Konferencja: 4th Jagiellonian Symposium on Advances in Particle Physics and Medicine, Krakow, 10-15 July 2022
• Języki publikacji: EN

Abstrakty

EN

Objectives: Extracellular vesicles (EVs) are heterogeneous membrane vesicles in diameter of 30-5000 nm, that transport proteins, non-coding RNAs (miRNAs), lipids and metabolites. Major populations include exosomes, ectosomes and apoptotic bodies. The purpose of this study was to compare the distribution of EVs obtained under different conditions of differential centrifugation, including ultracentrifugation, with the results developed based on a theoretical model. Methods: Immortalized endothelial cell line that expresses h-TERT (human telomerase) was used to release of EVs: microvascular TIME. EVs were isolated from the culture medium at different centrifugation parameters. The size distribution of the EVs was measured using TRPS technology on a qNano instrument. Surface markers were evaluated using flow cytometry. The isolated EV subpopulations were compared with the theoretical model developed by Livshits. Results: EVs isolated from endothelial cells show strong aggregating properties, which was confirmed by TEM, TRPS imaging and flow cytometry. Conclusions: Obtaining pure EV subpopulations is difficult because of the small differences in the diameter of ectosomes and exosomes, and the strong aggregating properties of EVs.

Słowa kluczowe

EN

ectosomes; exosomes; endothelial cells; differential centrifugation

• Wydawca: Uniwersytet Jagielloński - Collegium Medicum ; Index Copernicus Sp. z o.o.
• Czasopismo: Bio-Algorithms and Med-Systems
• Rocznik: 2022
• Tom: Vol. 18, no. 1
• Strony: 171--179
• Opis fizyczny: Bibliogr. 31 poz., rys., tab.

Twórcy

autor

Durak-Kozica Martyna

• Marian Smoluchowski Institute of Physics, Department of Medical Physics, Jagiellonian University, Krakow, Poland
• Marian Smoluchowski Institute of Physics Faculty of Physics, Astronomy and Applied Computer Science, Jagiellonian University, Krakow, Poland
• Centre for Theranostics, Jagiellonian University, Kraków, Poland

autor

Wróbel Andrzej

• Marian Smoluchowski Institute of Physics, Department of Medical Physics, Jagiellonian University, Krakow, Poland
• Marian Smoluchowski Institute of Physics Faculty of Physics, Astronomy and Applied Computer Science, Jagiellonian University, Krakow, Poland
• Centre for Theranostics, Jagiellonian University, Kraków, Poland

autor

Platt Mark

• Department of Chemistry, Loughborough University, Loughborough, United Kingdom

autor

Stępień Ewa Ł.

• Marian Smoluchowski Institute of Physics, Department of Medical Physics, Jagiellonian University, Krakow, Poland
• Marian Smoluchowski Institute of Physics Faculty of Physics, Astronomy and Applied Computer Science, Jagiellonian University, Krakow, Poland
• Centre for Theranostics, Jagiellonian University, Kraków, Poland
• Total-Body Jagiellonian-PET Laboratory, Jagiellonian University, Kraków, Poland

Bibliografia

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Uwagi

Opublikowane przez Sciendo. Opracowanie rekordu ze środków MEiN, umowa nr SONP/SP/546092/2022 w ramach programu "Społeczna odpowiedzialność nauki" - moduł: Popularyzacja nauki i promocja sportu (2022-2023).