The biomechanical characteristics of spinal dura mater in the context of its basic morphology

• Autorzy: Szotek Sylwia , Dawidowicz Joanna , Geniusz Malwina , Kozak Marta , Łukomski Robert , Czogalla Aleksander

Identyfikatory

DOI

10.37190/ABB-01972-2021-02

• Języki publikacji: EN

Abstrakty

EN

Purpose: Spinal dura mater plays a crucial role in the biomechanics and protection of the spine. Therefore, the present study investigated the dura mater's mechanical and basic morphological properties to learn more about the biomechanical behaviour of this fibrous membrane. Methods: Tissue strips, oriented in the longitudinal and circumferential directions, were cut from the cervical, thoracic, and lumbar vertebrae parts of the porcine spinal cord. Uniaxial tensile tests were performed using a device with a speed of 4 mm/min until rupture of the sample. Results: It was demonstrated that the dura mater is a heterogeneous, anisotropic material. The longitudinal excised specimens showed the highest values of mechanical properties (ultimate force (FU), the stiffness coefficient (k), ultimate tensile strength (σUTS), and Young’s modulus (E)) compared to those of the circumferentially. Confocal microscopy and sulforhodamine B (SRB) assay enabled us to visualise collagen and elastin elements more efficiently without a need for sample fixation. Conclusions: The spinal dura mater mechanical properties are not uniform along the entire length of the spinal cord, but, in the case of morphological features, no major differences were noticed. The utilisation of SRB occurred to be a non-destructive, fast, and efficient tool for visualising even the smallest elastic fibres on different depths of examined samples. The mechanical and morphological properties of the dura mater provided by this study can be further used in computational modelling to understand injury mechanisms better and help develop injury prevention strategies.

Słowa kluczowe

EN

spine; mechanical properties; collagen fibres; elastin fibres; confocal microscopy; sulforhodamine B

PL

kręgosłup; właściwości mechaniczne; włókna kolagenowe; włókna elastynowe; mikroskopia konfokalna; sulforodamina B

• Wydawca: Oficyna Wydawnicza Politechniki Wrocławskiej
• Czasopismo: Acta of Bioengineering and Biomechanics
• Rocznik: 2021
• Tom: Vol. 23, no. 4
• Strony: 149--159
• Opis fizyczny: Bibliogr. 39 poz., rys., tab.

Twórcy

autor

Szotek Sylwia

• Department of Mechanics, Materials and Biomedical Engineering, Faculty of Mechanical Engineering, Wrocław University of Science and Technology, Wrocław, Poland

autor

Dawidowicz Joanna

• Veterinary Clinic “Brynów”, Katowice, Poland

autor

Geniusz Malwina

• Department of Optics and Photonics, Faculty of Fundamental Problems of Technology, Wrocław University of Science and Technology, Wrocław, Poland

autor

Kozak Marta

• Department of Optics and Photonics, Faculty of Fundamental Problems of Technology, Wrocław University of Science and Technology, Wrocław, Poland

autor

Łukomski Robert

• Department of Electrical Power Engineering Faculty of Electrical Engineering, Wrocław University of Science and Technology, Wrocław, Poland

autor

Czogalla Aleksander

• Department of Cytobiochemistry, Faculty of Biotechnology, University of Wrocław, Wrocław, Poland

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Uwagi

Opracowanie rekordu ze środków MNiSW, umowa nr SONP/SP/546092/2022 w ramach programu "Społeczna odpowiedzialność nauki" - moduł: Popularyzacja nauki i promocja sportu (2024).