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Abstrakty
EN
The interaction between oppositely charged membranes and polycations causes cell aggregation, loss of membrane fluidity, and membrane degeneration and may cause an increase of its permeability. Unfortunately, the interaction is the reason why the use of polycations in medicine is severely limited. Therefore, in this paper, we share our observations related to the action of 40-kDa dextran modified using glycidyltrimethylammonium chloride, resulting in increased fibroblast cell proliferation. Using viability and proliferation tests [3-(4,5- dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide, crystal violet, 3H-thymidine incorporation], we have observed that cationic dextran derivatives exert a positive impact on nonepithelial cell proliferation in vitro. This phenomenon has been noted for human and mouse fibroblasts and several other nonepithelial cell lines. However, the effect seems to be most pronounced for fibroblast cell lines. The presented studies allow to examine the impact of the polymer structure and the methods of its cationic modification on this newly observed phenomenon. The observation is unique because positively charged macromolecules usually exhibit high toxicity in all cell types in vitro.
Rocznik
Strony
art. no. 20180036
Opis fizyczny
Bibliogr. 13 poz., rys., tab.
Twórcy
autor
  • Faculty of Chemistry, Jagiellonian University, Gronostajowa 2, 30-387 Krakow, Poland
  • Department of Cell Biochemistry, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 30-387 Krakow, Poland
autor
  • Faculty of Chemistry, Jagiellonian University, Gronostajowa 2, 30-387 Krakow, Poland
autor
  • Medical Biochemistry, Jagiellonian University Medical College, Kopernika 7C, 31-034 Krakow, Poland
  • Faculty of Chemistry, Jagiellonian University, Gronostajowa 2, 30-387 Krakow, Poland
  • Medical Biochemistry, Jagiellonian University Medical College, Kopernika 7C, 31-034 Krakow, Poland
Bibliografia
  • [1] Fischer D, Li Y, Ahlemeyer B, Krieglstein J, Kissel T. In vitro cytotoxicity testing of polycations: influence of polymer structure on cell viability and hemolysis. Biomaterials 2003;24:1121-31.
  • [2] Elferink JG. Changes of plasma membrane permeability in neutrophils treated with polycations. Inflammation 1991;15:103-15.
  • [3] Monnery BD, Wright M, Cavill R, Hoogenboom R, Shaunak S, Steinke JH, et al. Cytotoxicity of polycations: relationship of molecular weight and the hydrolytic theory of the mechanism of toxicity. Int J Pharm 2017;521:249-58.
  • [4] Kalaska B, Kaminski K, Sokolowska E, Czaplicki D, Kujdowicz M, Stalinska K, et al. Nonclinical evaluation of novel cationically modified polysaccharide antidotes for unfractionated heparin. PLoS One 2015;10:e0119486.
  • [5] Yudovin-Farber I, Gurt I, Hope R, Domb AJ, Katz E. Inhibition of herpes simplex virus by polyamines. Antivir Chem Chemother 2009;20:87-98.
  • [6] Marini JJ, Wheeler AP. Critical care medicine. Philadelphia: Wolters Kluwer/Lippincott Williams & Wilkins, 2010.
  • [7] Lewis SL. Medical surgical nursing. St. Louis, MI, USA: Elsevier - Mosby, 2017.
  • [8] Neu B, Wenby R, Meiselman HJ. Effects of dextran molecular weight on red blood cell aggregation. Biophys J 2008;95:3059-65.
  • [9] Rosenblum WI. Effects of dextran-40 on blood viscosity in experimental macroglobulinaemia. Nature 1968;218:591-3.
  • [10] Kalaska B, Sokolowska E, Kaminski K, Szczubialka K, Kramkowski K, Mogielnicki A, et al. Cationic derivative of dextran reverses anticoagulant activity of unfractionated heparin in animal models of arterial and venous thrombosis. Eur J Pharmacol 2012;686:81-9.
  • [11] Sznarkowska A, Olszewski R, Zawacka-Pankau J. Pharmacological activation of tumor suppressor, wild-type p53 as a promising strategy to fight cancer. Postępy Hig Med Dosw 2010;64:396-407.
  • [12] Tassi E, Al-Attar A, Aigner A, Swift MR, McDonnell K, Karavanov A, et al. Enhancement of fibroblast growth factor (FGF) activity by an FGF-binding protein. J Biol Chem 2001;276:40247-53.
  • [13] Chu H, Johanson NR, Mason NS, Wang Y. A [polycation:heparin] complex releases growth factors with enhanced bioactivity. J Control Release 2011;150:157-63.
Uwagi
Opracowanie rekordu w ramach umowy 509/P-DUN/2018 ze środków MNiSW przeznaczonych na działalność upowszechniającą naukę (2019).
Typ dokumentu
Bibliografia
Identyfikator YADDA
bwmeta1.element.baztech-09a2fb7b-1629-4c06-97bd-a70c3329bd52
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