Cell proliferation induced by modified cationic dextran

• Autorzy: Kamiński K. , Stalińska K. , Niziołek A. , Wróbel M. , Nowakowska M. , Kaczor-Kamińska M.

Identyfikatory

DOI

10.1515/bams-2018-0036

• Języki publikacji: EN

Abstrakty

EN

The interaction between oppositely charged membranes and polycations causes cell aggregation, loss of membrane fluidity, and membrane degeneration and may cause an increase of its permeability. Unfortunately, the interaction is the reason why the use of polycations in medicine is severely limited. Therefore, in this paper, we share our observations related to the action of 40-kDa dextran modified using glycidyltrimethylammonium chloride, resulting in increased fibroblast cell proliferation. Using viability and proliferation tests [3-(4,5- dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide, crystal violet, 3H-thymidine incorporation], we have observed that cationic dextran derivatives exert a positive impact on nonepithelial cell proliferation in vitro. This phenomenon has been noted for human and mouse fibroblasts and several other nonepithelial cell lines. However, the effect seems to be most pronounced for fibroblast cell lines. The presented studies allow to examine the impact of the polymer structure and the methods of its cationic modification on this newly observed phenomenon. The observation is unique because positively charged macromolecules usually exhibit high toxicity in all cell types in vitro.

Słowa kluczowe

EN

dextran derivative; nonepithelial cells; polycations; proproliferation effect

• Wydawca: Uniwersytet Jagielloński - Collegium Medicum ; De Gruyter
• Czasopismo: Bio-Algorithms and Med-Systems
• Rocznik: 2018
• Tom: Vol. 14, no. 4
• Strony: art. no. 20180036
• Opis fizyczny: Bibliogr. 13 poz., rys., tab.

Twórcy

autor

Kamiński K.

• Faculty of Chemistry, Jagiellonian University, Gronostajowa 2, 30-387 Krakow, Poland

autor

Stalińska K.

• Department of Cell Biochemistry, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 30-387 Krakow, Poland

autor

Niziołek A.

• Faculty of Chemistry, Jagiellonian University, Gronostajowa 2, 30-387 Krakow, Poland

autor

Wróbel M.

• Medical Biochemistry, Jagiellonian University Medical College, Kopernika 7C, 31-034 Krakow, Poland

autor

Nowakowska M.

• Faculty of Chemistry, Jagiellonian University, Gronostajowa 2, 30-387 Krakow, Poland

autor

Kaczor-Kamińska M.

• Medical Biochemistry, Jagiellonian University Medical College, Kopernika 7C, 31-034 Krakow, Poland

Bibliografia

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Uwagi

Opracowanie rekordu w ramach umowy 509/P-DUN/2018 ze środków MNiSW przeznaczonych na działalność upowszechniającą naukę (2019).