Simultaneous RP-IP-HPLC assay of theophylline, phenobarbital, codeine, and ephedrine in a suppository

• Autorzy: Jankovics P , Németh T , Németh-Palotás J , Kőszegi-Szalai H
• Języki publikacji: EN

Abstrakty

EN

A reversed-phase ion-pairing liquid chromatographic (RP-IP-HPLC) method for simultaneous assay of theophylline (TH), phenobarbital (PB), codeine (CD), and ephedrine (EP) in an extemporaneous (magistral) suppository was developed and validated and used to investigate a reported serious adverse event. Samples were dissolved in dichloromethane and extracted by two-step liquid-liquid extraction with acetate buffer (pH 5.0) and, subsequently, 0.1 M NaOH solution. Separation was performed on an end-capped C 18 silica column with stepwise gradient elution. Sample preparation and chromatographic conditions were optimized on the basis of the p K a values of the analytes. Freedom from interference from the excipients, linearity ( r ² > 0.999 for all the active ingredients), range (0.01–0.08 mg mL ^-1 for TH, CD, and EP; 0.0025–0.02 mg mL ^-1 for PB), intra-day and inter-day precision, and accuracy (recovery >95% for TH, CD, and EP and >90% for PB) of the method were demonstrated. Non-compliance of the examined product was confirmed.

Słowa kluczowe

EN

RP-IP-HPLC; suppository; theophylline; phenobarbital; codeine; ephedrine

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2010
• Tom: Vol. 22, no. 4
• Strony: 527--538
• Opis fizyczny: Bibliogr. 24 poz., rys., tab.

Twórcy

autor

Jankovics P

• National Institute of Pharmacy PO Box 450 1051 Budapest Hungary

autor

Németh T

• National Institute of Pharmacy PO Box 450 1051 Budapest Hungary

autor

Németh-Palotás J

• National Institute of Pharmacy PO Box 450 1051 Budapest Hungary

autor

Kőszegi-Szalai H

• National Institute of Pharmacy PO Box 450 1051 Budapest Hungary

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