Kinetic modelling of lovastatin biosynthesis by aspergillus terreus cultivated on lactose and glycerol as carbon sources

• Autorzy: Pawlak M. , Bizukojć M.
• Języki publikacji: EN

Abstrakty

EN

A kinetic model to describe lovastatin biosynthesis by Aspergillus terreus ATCC 20542 in a batch culture with the simultaneous use of lactose and glycerol as carbon sources was developed. In order to do this the kinetics of the process was first studied. Then, the model consisting of five ordinary differential equations to balance lactose, glycerol, organic nitrogen, lovastatin and biomass was proposed. A set of batch experiments with a varying lactose to glycerol ratio was used to finally establish the form of this model and find its parameters. The parameters were either directly determined from the experimental data (maximum biomass specific growth rate, yield coefficients) or identified with the use of the optimisation software. In the next step the model was verified with the use of the independent sets of data obtained from the bioreactor cultivations. In the end the parameters of the model were thoroughly discussed with regard to their biological sense. The fit of the model to the experimental data proved to be satisfactory and gave a new insight to develop various strategies of cultivation of A. terreus with the use of two substrates.

Słowa kluczowe

EN

lovastatin; multi-substrate kinetics; modeling; batch bioreactor

PL

lowastatyna; modelowanie; bioreaktor

• Wydawca: Komitet Inżynierii Chemicznej i Procesowej Polskiej Akademii Nauk
• Czasopismo: Chemical and Process Engineering
• Rocznik: 2012
• Tom: Vol. 33, nr 4
• Strony: 651--665
• Opis fizyczny: Bibliogr. 18 poz., rys., tab.

Twórcy

autor

Pawlak M.

• Łódź University of Technology, Faculty of Process and Environmental Engineering, Department of Bioprocess Engineering, Wolczanska 213, Lodz, Poland

autor

Bizukojć M.

• Łódź University of Technology, Faculty of Process and Environmental Engineering, Department of Bioprocess Engineering, Wolczanska 213, Lodz, Poland

Bibliografia

• 1. Bizukojć M., Ledakowicz S., 2010. The morphological and physiological evolution of Aspergillus terreus mycelium in the submerged culture and its relation to the formation of secondary metabolites. World J. Microbiol. Biotechnol. 26, 41-54. DOI: 10.1007/s11274-009-0140-1.
• 2. Bizukojć M., Ledakowicz S., 2007. A macrokinetic modelling of the biosynthesis of lovastatin by Aspergillus terreus. J. Biotechnol. 130, 422-435. DOI: 10.1016/j.jbiotec.2007.05.007.
• 3. Bizukojć M., Pawłowska B., Ledakowicz S., 2007. Supplementation of the cultivation media with B-group vitamins enhances lovastatin biosynthesis by Aspergillus terreus. J. Biotechnol. 127, 258-268. DOI: 10.1016/j.jbiotec.2006.06.017.
• 4. Bizukojć M., Pecyna M., 2011. Lovastatin and (+)-geodin formation by Aspergillus terreus ATCC 20542 in a batch culture with the simultaneous use of lactose and glycerol as carbon sources. Eng. Life Sci. 11, 272-282. DOI: 10.1002/elsc.201000179.
• 5. Casas López J.L., Sánchez Pérez J.A., Fernández Sevilla J.M., Acién Fernández F.G., Molina Grima E., Chisti Y., 2003. Production of lovastatin by Aspergillus terreus: effects of C:N ratio and the principal nutrients on growth and metabolite production. Enzyme Microb. Technol., 33, 270-277. DOI: 10.1016/S0141-0229(03)00130-3.
• 6. Kinetic modelling of lovastatin biosynthesis by Aspergillus terreus cultivated on lactose and glycerol...
• 7. Casas López J.L., Sánchez Pérez J.A., Fernández Sevilla J.M., Rodríguez Porcel E.M., Chisti Y., 2005. Pellet morphology, culture rheology and lovastatin production in cultures of Aspergillus terreus. J. Biotechnol., 116, 61-77. DOI: 10.1016/j.jbiotec.2004.10.005.
• 8. Hajjaj H., Niederberger P., Duboc P,. 2001. Lovastatin biosynthesis by Aspergillus terreus in a chemically defined medium. Appl. Environ. Microbiol., 67, 2596-2602. DOI: 10.1128/AEM.67.6.2596-2602.2001.
• 9. Kumar M.S., Jana S.K., Senthil V., Shashanka V., Kumar S.V. Sadhukhan A.K., 2000. Repeated fed-batch process for improving lovastatin production. Proc. Biochem. 36, 363-368. DOI: 10.1016/S0032-9592(00)00222
• 10. Lai L.-S.T., Tsai T.-H., Wang T.C., Cheng T.Y., 2005. The influence of culturing environments on lovastatin production by Aspergillus terreus in submerged cultures. Enz. Microb. Technol. 36, 737-748. DOI: 10.1016/j.enzmictec.2004.12.021.
• 11. Liu G., Xu Z., Cen P., 2000. A morphologically structured model for mycelial growth and secondary metabolite formation. Chin. J. Chem. Eng., 8, 46-51.
• 12. Manzoni, M., Rollini, M., Bergomi, S., Cavazzoni, V., 1998. Production and purification of statins from Aspergillus terreus strains. Biotechnol. Tech., 12, 529–532. DOI: 10.1023/A:1008851430560.
• 13. Metz B. Kossen N.W.F., 1977. The growth of molds in the form of pellets – A literature review. Biotechnol. Bioeng. 19, 555-564. DOI: 10.1002/bit.260190602.
• 14. Nielsen J., 2006. Microbial process kinetics, In: Ratledge C., Kristiansen B. (Eds.), Basic Biotechnology. 3rd edition, Cambridge University Press.
• 15. Nielsen J., Villadsen J., Liden G., 2003. Bioreaction engineering principles. Kluwer Academic/Plenum Publishers, New York.
• 16. Pawlak M., Bizukojć M., Ledakowicz S., 2012. Impact of bioreactor scale on lovastatin biosynthesis by Aspergillus terreus ATCC 20542 in a batch culture. Chem. Proc. Eng., 33, 71-84. DOI: 10.2478/v10176-0120007-0.
• 17. Pecyna M., Bizukojć M., 2011. Lovastatin biosynthesis by Aspergillus terreus with the simultaneous use of lactose and glycerol in a discontinuous fed-batch culture J. Biotechnol. 151, 77–86. DOI: 10.1016/j.jbiotec.2010.10.079.
• 18. Rodríguez Porcel E.M., Casas López J.L., Sánchez Pérez J.A., Fernández Sevilla J.M., García Sánchez J.L., Chisti Y., 2006. Aspergillus terreus broth rheology, oxygen transfer, and lovastatin production in a gas-agitated slurry reactor. Ind. Eng. Chem. Res. 45, 4837-4843. DOI: 10.1021/ie0600801.