Systematic evaluation of Rutongshu oral liquid based on chromatographic fingerprinting and nontargeted metabolomic analysis

• Autorzy: Fan Zheng , Xu Yuling , Lu Mengya , Yan Lanmeng , Du Fangping , Xu Jing , Tong Xiaohui , Han Rongchun

Identyfikatory

DOI

10.1556/1326.2023.01180

• Języki publikacji: EN

Abstrakty

EN

Based on chromatographic fingerprinting combined with quantitative analysis on characteristic chemical constituents as well as hierarchical cluster analysis, an easy and sensitive approach utilizing high performance liquid chromatography (HPLC) was developed for the identification and quality evaluation of Rutongshu oral liquid (ROL). What is more, nontargeted metabolomic analysis was conducted to gain a global view in terms of its chemical profile. In this study, 16 peaks from different batches (S1–S10) of ROL samples produced by Taihe Hospital of Chinese Medicine were selected as common peaks for the evaluation of their similarity whose values of all tested 10 batches exceeded 0.90 when compared with the control fingerprints. Meanwhile, simultaneous quantification of five markers in the oral solution, including albiflorin, paeoniflorin, chlorogenic acid, quercetin and ferulic acid was performed, and standard curves established for respective reference substances showed good regression in the linear range (r2 > 0.999) with recoveries in the range of 98.96–102.35%. The ultra-high performance liquid chromatography (UHPLC) combined with Orbitrap Exploris 120 mass spectrometer resulted in 88 identified compounds. The results of validation showed that the established method was reproducible, precise and stable. This study offers an effective, dependable and useful approach for the systematic evaluation of the hospital formulation ROL.

Słowa kluczowe

EN

Rutongshu oral liquid; quality evaluation; HPLC; metabolomic analysis; chromatographic fingerprinting

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2024
• Tom: Vol. 36, no. 4
• Strony: 383--394
• Opis fizyczny: Bibliogr. 38 poz., tab., wykr.

Twórcy

autor

Fan Zheng

• Affiliated Taihe Hospital of Chinese Medicine, Anhui University of Chinese Medicine, Taihe 236600, China

autor

Xu Yuling

• School of Pharmacy, Anhui University of Chinese Medicine, Hefei 230012, China

autor

Lu Mengya

• Affiliated Taihe Hospital of Chinese Medicine, Anhui University of Chinese Medicine, Taihe 236600, China

autor

Yan Lanmeng

• School of Pharmacy, Anhui University of Chinese Medicine, Hefei 230012, China

autor

Du Fangping

• Jinzhai County Jinshanzhai Edible and Pharmaceutical Fungi Plantation Co. Ltd., Jinzhai 237300, China

autor

Xu Jing

• School of Life Sciences, Anhui University of Chinese Medicine, Hefei 230012, China

autor

Tong Xiaohui

• School of Life Sciences, Anhui University of Chinese Medicine, Hefei 230012, China
• Functional Activity and Resource Utilization on Edible and Medicinal Fungi Joint Laboratory of Anhui Province, Jinzhai 237300, China

• https://orcid.org/0000-0001-8815-1230

autor

Han Rongchun

• School of Pharmacy, Anhui University of Chinese Medicine, Hefei 230012, China
• Joint Research Center for Chinese Herbal Medicine of Anhui of IHM, Anhui University of Chinese Medicine, Hefei 230012, China

• https://orcid.org/0000-0001-7520-9413

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