Development and validation of a gradient elution high-performance liquid chromatographic method for determination of talinolol in rat plasma: Application to a preclinical food-drug interaction study

• Autorzy: Mahgoub H , Hanafy A. , Bamane F. H. , Radwan A. E.
• Języki publikacji: EN

Abstrakty

EN

A sensitive and reproducible high-performance liquid chromatographic (HPLC) method for determination of talinolol (TAL) in rat plasma was developed and validated using pindolol (PIN) as an internal standard. Both TAL and PIN were separated on a Zorbax Eclipse XDB C18 column by gradient elution with 0.1% aqueous formic acid and acetonitrile as the mobile phase. Detection was performed using fluorescence measurement at λex 249 nm and λem 333 nm. The method was validated and found to be linear in the range of 10–2000 ng mL−1. The limit of quantification was 10 ng mL−1 based on 100 μL of plasma. The variations for intra- and inter-day precision were less than 10%, and the accuracy values were between 92% and 102.9%. The extraction recoveries were more than 82%. The assay was successfully applied to an in-vivo pharmacokinetic study of TAL in rats that were administered a single oral dose of 10 mg kg−1 TAL. The maximum concentration (Cmax) and the area under the plasma concentration-time curve (AUC0–12) were 0.369 ± 0.024 μg mL−1 and 1.429 ± 0.027 μg h mL−1, respectively. The modulatory effect of apricot juice on P-glycoprotein-related efflux carriers was also investigated. Co-administration of apricot juice resulted in a significant increase of the amount of TAL in plasma (Cmax and AUC0–12 were 0.679 ± 0.021 and 2.357 ± 0.079, respectively; p < 0.05).

Słowa kluczowe

EN

talinolol; HPLC; gradient elution; rat plasma; pharmacokinetic study; food-drug interaction; apricot juice; P-gp

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2015
• Tom: Vol. 27, no. 1
• Strony: 67--79
• Opis fizyczny: Bibliogr. 25 poz., rys., tab.

Twórcy

autor

Mahgoub H

• King Abdulaziz University Department of Pharmaceutical Chemistry, Faculty of Pharmacy Jeddah Saudi Arabia
• University of Alexandria Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy Alexandria Egypt

autor

Hanafy A.

• King Abdulaziz University Department of Pharmacology and Toxicology, Faculty of Pharmacy Jeddah Saudi Arabia
• Kafrelsheikh University Department of Pharmacology, Faculty of Veterinary Medicine Kafrelsheikh Egypt

autor

Bamane F. H.

• King Abdulaziz University Department of Biochemistry, Faculty of Medicine Jeddah Saudi Arabia

autor

Radwan A. E.

• University of Alexandria Department of Biotechnology, Institute of Graduate Studies and Research Alexandria Egypt

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