A simple and reliable preparative high-performance liquid chromatographic technique for isolation of a bioactive flavone diglycoside from and extract of Cuminum cyminum seeds

• Autorzy: Satti N. K , Amina M , Dutt P , Sharma V. K. , Sharma P. , Khan I. , Gupta B. D , Suri K. A , Sharma S. C. , Johri R. K , Sharma S. N
• Języki publikacji: EN

Abstrakty

EN

In this paper we describe a sensitive and reproducible reversed-phase high-performance liquid chromatography (HPLC) method with photodiode-array detection for isolation and quantification of the bioactive hydrophilic constituent 7-(1- O -β-D -galacturonide-4′-(1- O -β-D -glucopyranosyl)-3′,4′,5,7-tetrahydroxyflavone, 1 , from the seeds of Cuminum cyminum . Compound 1 was separated isocratically on a C 18 preparative column, in high purity, after removal of solvents. The purity and identity of the compound were established by use of LC-mass spectrometry and by spectroscopic techniques ( 1 H and 13 C NMR). The purity of 1 was also confirmed by HPTLC.

Słowa kluczowe

EN

Cuminum cyminum seeds; flavones diglycoside; Prep. HPLC; PDA; HPTLC; LCMS

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2009
• Tom: Vol. 21, no. 3
• Strony: 499--512
• Opis fizyczny: Bibliogr. 13 poz., rys., tab.

Twórcy

autor

Satti N. K

• Indian Institute of Integrative Medicine (CSIR) Natural Products Chemistry Division Canal Road Jammu Tawi 180001 India

autor

Amina M

• Indian Institute of Integrative Medicine (CSIR) Natural Products Chemistry Division Canal Road Jammu Tawi 180001 India

autor

Dutt P

• Indian Institute of Integrative Medicine (CSIR) Natural Products Chemistry Division Canal Road Jammu Tawi 180001 India

autor

Sharma V. K.

• Indian Institute of Integrative Medicine (CSIR) Natural Products Chemistry Division Canal Road Jammu Tawi 180001 India

autor

Sharma P.

• Indian Institute of Integrative Medicine (CSIR) Natural Products Chemistry Division Canal Road Jammu Tawi 180001 India

autor

Khan I.

• Indian Institute of Integrative Medicine (CSIR) Natural Products Chemistry Division Canal Road Jammu Tawi 180001 India

autor

Gupta B. D

• Indian Institute of Integrative Medicine (CSIR) Natural Products Chemistry Division Canal Road Jammu Tawi 180001 India

autor

Suri K. A

• Indian Institute of Integrative Medicine (CSIR) Natural Products Chemistry Division Canal Road Jammu Tawi 180001 India

autor

Sharma S. C.

• Indian Institute of Integrative Medicine (CSIR) Pharmacology Division Canal Road Jammu Tawi 180001 India

autor

Johri R. K

• Indian Institute of Integrative Medicine (CSIR) Pharmacology Division Canal Road Jammu Tawi 180001 India

autor

Sharma S. N

• Indian Institute of Integrative Medicine (CSIR) Plant Survey Division Canal Road Jammu Tawi 180001 India

Bibliografia

• [1] G. Charalambous, Spices, Herbs and Edible Fungi, Elsevier, Amsterdam, 1994
• [2] A. Zargari, Medicinal Plants, Vol. II, Tehran University Press, Tehran, 1989, p. 519
• [3] S.G. Joshi,. Medical Plants: Family Apicaceae, 1st edn, IBH Publishing, Oxford, 2000, p. 34
• [4] S.A. Willatgamuwa, K. Platel, G. Sarawathi, and K. Srinivasan, Nutr. Res., 18 , 131 (1998)
• [5] T. Malini and G. Vanithakumari, Indian J. Exp. Biol., 5 , 442 (1987)
• [6] M. Sayyah, A. Mahboubi, and M. Kamalinejad, Pharm. Biol., 40 , 47 (2002)
• [7] M. Sayyah, A. Peirovi, and M. Kamalinejad, Iran Biomed. J., 6 , 141 (2002)
• [8] K.C. Srivastava, Prostaglandins Leukotrienes Essential Fatty Acids, 37 , 57 (1989)
• [9] G.N. Qazi, K.L. Bedi, R.K. Johri, M.K. Tikoo, A.K. Tikoo, S.C. Sharma, S.A. Tasduq, O.P. Suri, B.D. Gupta, K.A. Suri, N.K. Satti, A.K. Kalsotra, and R.K. Khajuria, US Patent 7,070,814(2006)
• [10] G.N. Qazi, O.P. Suri, K.L. Bedi, I. A. Khan, V. Verma, R.K. Johri, K.A. Suri, B.D. Gupta, N.K. Satti, M.K. Tikoo, S.C. Sharma, and A.K. Tikoo, US Patent 7119075 (2006)
• [11] G.N. Qazi, K.L. Bedi, R.K. Johri, S.C. Sharma, M.K. Tikoo, A.K. Tikoo, S.A. Tasduq, K. Singh, R. Pandita, O.P. Suri, B.D. Gupta, K.A. Suri, N.K. Satti, R.K. Khajuria, B.K. Kapahi, and A.K. Kalsotra, US Patent filed; Indian Patent NF-292/2002
• [12] B.S. Sachin, S.C. Sharma, S. Sethi, S.A. Tasduq, M.K. Tikoo, A.K. Tikoo, N.K. Satti, B.D. Gupta, KA. Suri, R.K. Johri, and G.N. Qazi, Phytother Res., 21 , 157 (2007)
• [13] S.I. El-Negoumy and R.M.A. Mansour, Grasas Aceites (Seville), 40 , 87 (1989)