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Dichloromethane-Extract of Propolis (DEP) and DEP/PLA Electrospun Fiber Membranes

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Treść / Zawartość
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Warianty tytułu
PL
Zastosowanie ekstrakt z propolisu (DEP) i do wytworzenia elektroprzędzonych membran światłowodowych DEP/PLA
Języki publikacji
EN
Abstrakty
EN
Propolis is a waxy substance produced by the honeybee that has been used as a form of traditional medicine and natural medicine since ancient times. Propolis has a wide spectrum of alleged applications, including potential anti-infection and anti-cancer effects. The following paper used a propolis extract containing 90% ethanol solution, 70% ethanol solution, ligarine, and dichloromethane as solvents that extracted the bioactive components. The highest yield of the propolis was obtained via the 70% ethanol leaching method and dichloromethane immersion stirring method. Fourier Transform Infrared (FTIR) analysis proved that the extracted propolis with dichloromethane had the highest methylene content and the maximum types of effective propolis components. A Propolis/PLA electrospinning solution was prepared by adding PLA powder into the supernatant of the dichloromethane-extract of propolis (DEP) directly, with there being no need for purification of the propolis extract and thus reducing the loss of active ingredients. DEP/PLA nanofiber was prepared via the electrospinning process, where it was found that with additional 4% PLA, the final electrospun fiber membrane was stabilised. tStudy of the antibacterial performance of the DEP/PLA electrospun membrane showed that the membrane affected some of the antibacterial properties. It was particularly effective when inhibiting Staphylococcus aureus, but not as effective when inhibiting Escherichia coli. This electrostatic spinning membrane could be used for food preservation, wound healing, and tissue engineering.
PL
Propolis to woskowa substancja wytwarzana przez pszczołę miodną, która od starożytności była stosowana jako forma tradycyjnej medycyny i medycyny naturalnej. Propolis ma szerokie spektrum domniemanych zastosowań, w tym potencjalne działanie przeciwinfekcyjne i przeciwnowotworowe. W pracy użyto ekstraktu propolisu zawierającego 90% roztwór etanolu, 70% roztwór etanolu, ligarinę i dichlorometan jako rozpuszczalniki, które ekstrahowały składniki bioaktywne. Najwyższą wydajność propolisu uzyskano metodą wymywania 70% etanolu i metodą mieszania zanurzeniowego w dichlorometanie. Analiza w podczerwieni z transformacją Fouriera (FTIR) wykazała, że wyekstrahowany propolis z dichlorometanem miał najwyższą zawartość metylenu i dużą skuteczność. Roztwór propolis/PLA przygotowano przez dodanie proszku PLA bezpośrednio do ekstraktu z dichlorometanu propolisu (DEP), bez potrzeby oczyszczania, tym sposobem zmniejszono utraty składników aktywnych. Nanowłókno DEP/PLA zostało przygotowane w procesie elektroprzędzenia, w którym stwierdzono, że przy 4% PLA membrana z włókien elektroprzędzonych jest stabilna. Ocena skuteczności antybakteryjnej membrany DEP/PLA wykazała, że posiada ona właściwości przeciwbakteryjne. Stwierdzono zadowalającą skuteczność w hamowaniu Staphylococcus aureus, przy jednocześnie niższej skuteczności w hamowaniu Escherichia coli. Wytworzona membrana może być używana do konserwacji żywności, gojenia się ran i w inżynierii tkankowej.
Rocznik
Strony
57--62
Opis fizyczny
Bibliogr. 35 poz., rys., tab.
Twórcy
autor
  • Minjiang University, Fujian Key Laboratory of Novel Functional Textile Fibers and Materials, Fuzhou, 350108, P.R. China
autor
  • Minjiang University, Clothing and Design Faculty, Fuzhou, Fujian 350121, P.R. China
autor
autor
  • Minjiang University, Clothing and Design Faculty, Fuzhou, Fujian 350121, P.R. China
Bibliografia
  • 1. Eroglu N, Akkus S, Yaman M, Asci B, Silici S. Amino Acid and Vitamin Content of Propolis Collected by Native Caucasican Honeybees. Journal of Apicultural Science 2016; 60(2): 101-110.
  • 2. PengBo G, Xiang X, LiMiao G, LiPing S. Research progress in chemical component of propolis and its geographic origins and plant origins. Journal of Food Safety and Quality 2015; 6(8): 3172-3176.
  • 3. Wilson M B, Brinkman D, Spivak M, Gardner G, Cohen J D. Regional variation in composition and antimicrobial activity of US propolis against Paenibacillus larvae and Ascosphaera apis. Journal of Invertebrate Pathology 2015; 124: 44- 50.
  • 4. Bueno-Silva B, Marsola A, Ikegaki M, Alencar S M, Rosalen P L. The effect of seasons on Brazilian red propolis and its botanical source: chemical composition and antibacterial activity. Natural Product Research 2017; 31(11): 1318-1324.
  • 5. Li A, Xuan H, Sun A, Liu R, Cui J. Preparative separation of polyphenols from water-soluble fraction of Chinese propolis using macroporous absorptive resin coupled with preparative high performance liquid chromatography. Journal of Chromatography B 2016; 1012: 42-49.
  • 6. Falcão S I, Tomás A, Freire C, Vilas-Boas M. A voltammetric tool for the evaluation of propolis antioxidant activity. European Food Research and Technology 2016; 242(8): 1393-1401.
  • 7. Martini D, Barbosa G F, Matias R, Marques Filho W C, Garcia N Z T. Seasonality on the antifungal potential of green propolis collected in Campo Grande-MS, Brazil. Ciência Rural 2017; 47(3): 457-468.
  • 8. Rai N, Rai K K, Venkataravanappa V, Saha S. Molecular Approach Coupled with Biochemical Attributes to Elucidate the Presence of DYMV in Leaf Samples of Lablab purpureus. L Genotypes. Applied Biochemistry and Biotechnology 2016; 178(5): 876-890.
  • 9. Mohamed E F, Owayss A A. An inhibitory activity of propolis extract against BroadBean Mottle Bromovirus (BBMV). International Journal of Virology 2005; 1(1): 31-31.
  • 10. Shimizu T, Hino A, Tsutsumi A, Park Y K, Watanabe W, Kurokawa M. Antiinfluenza virus activity of propolis in vitro and its efficacy against influenza infection in mice. Antiviral Chemistry and Chemotherapy 2008; 19(1), 7-13.
  • 11. Ramanauskiene K, Inkeniene A M, Savickas ARÛNAS, Masteikova R, Brusokas WALDEMARS. Analysis of the antimicrobial activity of propolis and lysozyme in semisolid emulsion systems. Acta Pol Pharm. 2009; 66(6): 681- 688.
  • 12. Ertürk Ö, Çil E, Yoloğlu N, Yavuz, C. An In vitro Study on Antimicrobial and Antioxidant Activity of Propolis from Rize Province of Turkey. Mellifera 2016; 16(1): 4-18.
  • 13. Adomavičiūtė E, Stanys S, Žilius M, et al. Formation and analysis of electrospun nonwoven mats from bicomponent PVA/aqueous propolis nano-microfibres[J]. FIBRES & TEXTILES in Eastern Europe 2015; 5 (113): 35--41. Nr DOI: 10.5604/12303666.1161754
  • 14. Ghisalberti E L. Propolis: a review. Bee World 1979; 60(2): 59-84.
  • 15. Gallenkemper G., Rabe E, Bauer R. Contact sensitization in chronic venous insufficiency: modern wound dressings. Contact Dermatitis 1998; 38(5): 274- 278.
  • 16. Onlen Y, Duran N, Atik E, Savas L, Altug E, Yakan S, Aslantas O. Antibacterial activity of propolis against MRSA and synergism with topical mupirocin. The Journal of Alternative and Complementary Medicine 2007; 13(7): 713-718.
  • 17. Celli N, Dragani L K, Murzilli S, et al. In vitro and in vivo stability of caffeic acid phenethyl ester, a bioactive compound of propolis[J]. Journal of Agricultural and Food Chemistry 2007; 55(9): 3398-3407.
  • 18. Cigut T, Polak T, Gašperlin L, et al. Antioxidative activity of propolis extract in yeast cells[J]. Journal of Agricultural and Food Chemistry 2011; 59(21): 11449- 11455.
  • 19. Avcı Ç B, Gündüz C, Baran Y, et al. Caffeic acid phenethyl ester triggers apoptosis through induction of loss of mitochondrial membrane potential in CCRF-CEM cells[J]. Journal of Cancer Research and Clinical Oncology 2011; 137(1): 41-47.
  • 20. Lee I K, Han M S, Kim D W, et al. Phenylpropanoid acid esters from Korean propolis and their antioxidant activities[J]. Bioorganic & Medicinal Chemistry Letters 2014; 24(15): 3503-3505.
  • 21. Velazquez C, Navarro M, Acosta A, et al. Antibacterial and free‐radical scavenging activities of Sonoran propolis[J]. Journal of Applied Microbiology, 2007, 103(5): 1747-1756.
  • 22. Abubakar Murtala B, et al. "Polyphenols as key players for the antileukaemic effects of propolis. Evidence-Based Complementary and Alternative Medicine2014.
  • 23. Ferguson L R. Role of plant polyphenols in genomic stability[J]. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis 2001; 475(1): 89-111.
  • 24. Asawahame C, Sutjarittangtham K, Eitssayeam S, et al. Antibacterial activity and inhibition of adherence of Streptococcus mutans by propolis electrospun fibers[J]. Aaps Pharmscitech 2015; 16(1): 182-191.
  • 25. Sutjarittangtham K, Tunkasiri T, Chantawannakul P, et al. Mechanically improved antibacterial polycaprolactone/propolis electrospun fiber mat by adding bacterial nanocellulose[J]. Journal of Computational and Theoretical Nanoscience 2015; 12(5): 798-803.
  • 26. Kim J I, Pant H R, Sim H J, et al. Electrospun propolis/polyurethane composite nanofibers for biomedical applications[J]. Materials Science and Engineering: C, 2014; 44: 52-57.
  • 27. Sutjarittangtham K, Sanpa S, Tunkasiri T, et al. Bactericidal effects of propolis/polylactic acid (PLA) nanofibres obtained via electrospinning[J]. Journal of Apicultural Research 2014, 53(1): 109-115.
  • 28. Asawahame C, Sutjarittangtham K, Eitssayeam S, et al. Formation of orally fast dissolving fibers containing propolis by electrospinning technique[J]. Chiang Mai Journal of Science 2015; 42: 469-480
  • 29. Sanpa S, Sutjarittangtham K, Tunkasiri T, et al. Antimicrobial effect of brazillian propolis/polycaprolactone polymer on some human pathogenic bacteria[C]//Advanced Materials Research. Trans Tech Publications 2012; 506: 537-540.
  • 30. Bonadies I, Cimino F, Ambrogi V, et al. Electrospun Drug-Loaded Textiles for Biomedical and Healthcare Applications[C]//Advances in Science and Technology. Trans Tech Publications 2017; 100: 64-72.
  • 31. Arıkan H K, Solak H H. Propolis Extract-PVA Nanocomposites of Textile Design: Antimicrobial Effect on Gram Positive and Negative Bacterias[J]. International Journal of Secondary Metabolite (IJSM) 2017; 4(3-1).
  • 32. Adomavičiūtė E, Stanys S, Žilius M, et al. Formation and biopharmaceutical characterization of electrospun PVP mats with propolis and silver nanoparticles for fast releasing wound dressing[J]. BioMed research international, 2016.
  • 33. Pujirahayu, NIKEN, Ritonga HALIMAHTUS. SADIYAH., & Uslinawaty, ZAKIAH. Properties and flavonoids content in propolis of some extraction method of raw propolis. Int J Pharm Pharm Sci. 2014;6:338-340.
  • 34. Zhang Y N, Ning Z L, Chen C W, Wang D W. Synergistic antimicrobial effect of ethanol extracts from clove and licorice. Food Science 2010; 31(21): 65-69.
  • 35. Kalogeropoulos N, Konteles S J, Troullidou E, Mourtzinos I. Karathanos V T. Chemical composition, antioxidant activity and antimicrobial properties of propolis extracts from Greece and Cyprus. Food Chemistry 2009; 116(2): 452- 461.
Uwagi
PL
Opracowanie rekordu w ramach umowy 509/P-DUN/2018 ze środków MNiSW przeznaczonych na działalność upowszechniającą naukę (2019).
Typ dokumentu
Bibliografia
Identyfikator YADDA
bwmeta1.element.baztech-06fb46d9-7bf0-4c58-917a-4eb9d74e4779
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