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Determination of optimum temperatures and activationenergies of inulin hydrolysis by endo-inulinase Aspergillus Niger

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Języki publikacji
EN
Abstrakty
EN
The paper presents a comparative analysis to determine the optimal temperatures and the activationenergies for various origin endo-inulinases fromAspergillus niger. The parameters were estimatedbased on the literature of the activity curves vs. temperature for hydrolysis of inulin. It was assumedthat both the hydrolysis reaction process and the deactivation process of endo-inulinase were first-orderreactions by the enzyme concentration. A mathematical model describing the effect of temperature onendo-inulinases fromAspergillus nigeractivity was used. Based on the comparison analysis, valuesof the activation energies Ea were in the range from23:53+/-3:20kJ/mol to50:66+/-3:61kJ/mol, thedeactivation energies Ed were in the range from88:42+/-5:03kJ/mol to142:87+/-2:75kJ/mol andthe optimum temperatures Topt were obtained in the range from317:12+/-0:83K to332:55+/-0:72forendo-inulinase A. niger.
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Strony
229--–236
Opis fizyczny
Bibliogr. 16 poz., rys., tab.
Twórcy
  • Department of Chemical and Biochemical Engineering,Faculty of Chemical Technology and Engineering, University of Science and Technology in Bydgoszcz, Seminaryjna 3, 85-326 Bydgoszcz, Poland
Bibliografia
  • 1. Chi Z. M., Zhang T., Cao T.S., Liu X. Y., Cui W., Zhao C. H., 2011. Biotechnological potential of inulin for bioprocesses. Bioresour. Technol., 102, 4295–4303. DOI: 10.1016/j.biortech.2010.12.086.
  • 2. Chi Z., Chi Z., Zhang T., Liu G., Yue L., 2009. Inulinase-expressing microorganisms and applications of inulinases. Appl. Microbiol. Biotechnol., 82, 211–220. DOI: 10.1007/s00253-008-1827-1.
  • 3. Karimi M., Chaudhury I., Jianjun C., Safari M., Sadeghi R., Habibi-Rezaei M., Kokini J., 2014. Immobilization of endo-inulinase on non-porous aminofunctionalized silica nanoparticles. J. Molecular Catalysis B: Enzymatic., 104, 48–55. DOI: 10.1016/j.molcatb.2014.01.025.
  • 4. Megazyme, 2000. Endo inulinase Aspergillus niger. (Lot 121002b). Available at: https://www.megazyme.com/ documents/Booklet/E-ENDOIAN_DATA.pdf.
  • 5. Miłek J., 2018. Estimation of the kinetic parameters for H2O2 enzymatic decomposition and for catalase deactivation. Braz. J. Chem. Eng., 35, 995–1004. DOI: 10.1590/0104-6632.20180353s20160617.
  • 6. Miłek J., 2020a. Calculation of temperature optimum as well as activation and deactivation energy for the olive oil hydrolysis with porcine pancreas lipase. Przem. Chem., 99, 585–587. DOI: 10.15199/62.2020.4.14.
  • 7. Miłek J., 2020b. Determination the optimum temperature and activation energies for the hydrolysis of starch by α-amylase Bacillus licheniformis. Przem. Chem., 99, 880–881. DOI: 10.15199/62.2020.6.9.
  • 8. Nguyen Q. D., Rezessy-Szabo J. M., Czukor B., Hoschke A., 2011. Continuous production of oligofructose syrup from Jerusalem artichoke juice by immobilized endo-inulinase. Process Biochem., 46, 298–303. DOI: 10.1016/ j.procbio.2010.08.028.
  • 9. Ohta K., Akimoto H., Moriyama S., 2004. Fungal inulinases: Enzymology, molecular biology and biotechnology. J. Appl. Glycosci., 51, 247–25. DOI: 10.5458/JAG.51.247.
  • 10. Ricca E., Calabrò V., Curcio S., Iorio G., 2009. Optimization of inulin hydrolysis by inulinase accounting for enzyme time- and temperature-dependent deactivation. Biochem. Eng. J., 48, 81–86. DOI: 10.1016/j.bej.2009.08.009.
  • 11. Singh P., Gill P.K., 2006. Production of inulinases: recent advances. Food Technol. Biotechnol., 44,151–162.
  • 12. Singh R. S., Chauhan K., 2018. Production, purification, characterization and applications of fungal inulinases. Current Biotechnol., 7, 242–260. DOI: 10.2174/2211550105666160512142330.
  • 13. Skowronek M., Fiedurek J., 2006. Purification and properties of extracellular endoinulinase from Aspergillus niger 20 OSM. Food Technol. Biotechnol., 44, 53–58.
  • 14. Wojcik M., Miłek J., 2016. A new method to determine optimum temperature and activation energies for enzymatic reactions. Bioprocess Biosyst. Eng., 39, 1319–1323. DOI: 10.1007/s00449-016-1596-7.
  • 15. Xu Y., Zheng Z., Xu Q., Yong Q., Ouyang J., 2016. Efficient conversion of inulin to inulooligosaccharides through endoinulinase from Aspergillus niger. J. Agric. Food Chem., 64, 2612–2618. DOI: 10.1021/acs.jafc.5b05908.
  • 16. Zaita N., Fukushige T., Tokuda M., Ohta K., Nakamura T., 2000. Preparation and enzymatic properties of onto various polysaccharide supports. Food Sci. Technol. Res., 6, 34–39.
Uwagi
Opracowanie rekordu ze środków MNiSW, umowa Nr 461252 w ramach programu "Społeczna odpowiedzialność nauki" - moduł: Popularyzacja nauki i promocja sportu (2020).
Typ dokumentu
Bibliografia
Identyfikator YADDA
bwmeta1.element.baztech-05d0bf69-91f5-41cf-b94c-24f921abad5f
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