Rapid and sensitive analysis of baclofen by high-performance liquid chromatography with UV-Vis and FD detection

• Autorzy: Cao L.-W , Li C.
• Języki publikacji: EN

Abstrakty

EN

Two rapid, sensitive and reproducible methods for the determination of baclofen( BAL) in urine and plasma based on high-performance liquid chromatography (HPLC) with UV-vis and fluorescent detection, respectively, were developed for the first time using a new synthesized fluorescent label, 6-oxy-(N-succinimidylacetate)-9-(2′-methoxycarbonyl) fluorescein (SAMF). The optimal derivatization yield was achieved in borate buffer (pH 8.0) for 15 min at room temperature (25 °C). With a mixture of methanol and water containing 5 mmol L^-1 sodium citrate buffer (pH 5.0) as mobile phase, BAL was determined at λ = 455 nm with UV-vis detection and at λex/λem = 488/520 nm with FD detection. The detection limits are 1.065 × 10^-3 mg mL^-1 and 1.065 × 10^-2 mg mL^-1 with HPLC-UV-vis and HPLC-FD, respectively. The proposed method has been successfully applied to the analysis of BAL in human urine and plasma samples. The established method is rapid (15 min of derivatization process and 10 min of chromatographic run), reproducible and sensitive.

Słowa kluczowe

EN

baclofen; 6-oxy-(N-succinimidyl acetate)-9-(2′-methoxycarbonyl) fluorescein; high-performance liquid chromatography; UV-vis detection; fluorescent detection

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2012
• Tom: Vol. 24, no. 3
• Strony: 383--397
• Opis fizyczny: Bibliogr. 20 poz., rys., tab.

Twórcy

autor

Cao L.-W

• Jinan University Department of Chemistry Guangzhou 510632 People’s Republic of China

autor

Li C.

• Jinan University Department of Chemistry Guangzhou 510632 People’s Republic of China

Bibliografia

• [1] H.Y. Aboul-Enein and I.W. Wainer, The Impact of Stereochemistry on Drug Development and Use, John Wiley & Sons, New York, NY, 1997, p. 58
• [2] R.I. Stefan van Staden and A.A Rat’k. Talanta, 69, 1049–1053 (2006)
• [3] S.J Hosseinimehr, F. Pourmorad, E. Moshtaghi, and M. Amini, Asian J. Che, 22, 522–526 (2010)
• [4] H.M. Saleh, M.M. EL-Henawee, G.H. Ragab, and S.S. El-Hay Abd, Spectrochim. Acta, Part A, 67, 1284–1289 (2007)
• [5] A. Rat’ko and R. Stefan-van Staden, IL FARMACO, 59, 993–997 (2004)
• [6] R. Bhushan and V. Kumar, Chromatographia, 22, 906–911 (2008)
• [7] A. Siouffi, G. Kaiser, and F. Leroux, J. Chromatogr., 450, 221–232 (1988)
• [8] M.M. Hefnawy and H.Y. Aboul-Enein, Talanta, 61, 667–673 (2003)
• [9] C.Y. Zhang, M. Yuan, B.Y. Huang, H. Ji, and L. Zhu, West Chin. J. Pharm. Sci., 23, 342–343 (2008)
• [10] S. Tosunoglu and L. Ersoy, Analyst, 120, 373–375 (1995)
• [11] G. Kavran-Belin, S. Rudaz, and J.L. Veuthey, J. Sep. Sci., 28, 2187–2192 (2005)
• [12] Y.S. Gu and C.W. Whang, J. Chromatogr. A, 972, 289–293 (2002)
• [13] Miksa and R. Poppenga, J. Anal. Toxicol., 27, 275–283 (2004)
• [14] L. Millerioux, M. Brault, V. Gualano, and A. Mignot, J. Chromatogr. A, 729, 309–314 (1996)
• [15] S. Chang and H. Yang, Chromatographia, 57, 825–829 (2003)
• [16] M.T. Chiang, S.Y. Chang and C.W. Whang, J. Chromatogr. A, 877, 233–237 (2000)
• [17] L.W. Cao, H.S. Zhang, and H. Wang, Microchim. Acta, 158, 361–368 (2007)
• [18] L.W. Cao, H. Wang, J.S. Li, and H.S. Zhang, J. Chromatogr. A, 1063, 143–151 (2005)
• [19] V.C. Wu, S.L. Lin, S.M. Lin, and C.C. Fang, Nephrol. Dial. Transpl., 20, 441–443 (2005)
• [20] R.P. Haugl, The Handbook. A Guide to Fluorescent Probes and Labeling Technologies, 10th edn, Molecular Probes Inc, Eugene, OR, 2005, p. 1