Development and validation of HPLC assay method for marbofloxacin determination in veterinary chewable tablets

• Autorzy: Oliveira K. R. W. , Sversut R. A. , Singh A. K. , Amaral M. S. , Kassab N. M.

Identyfikatory

DOI

10.1556/1326.2018.30305

• Języki publikacji: EN

Abstrakty

EN

The present study aimed to develop and validate an analytical method for determination of marbofloxacin (MAR) in veterinary chewable tablets. The isocratic reversed-phase chromatographic method was developed and validated using a Vertisep®, RP C18 column (150 mm × 4.6 mm, 5.0 μm). The mobile phase was composed of water–acetonitrile (55:45, v/v) with pH adjusted to 3.0 with ortho-phosphoric acid and a flow rate set at 0.4 mL/min. The proposed method was validated for linearity in a concentration range of 2.5 to 17.5 μg/mL with a correlation coefficient of 0.99991. The mean content of MAR found in chewable tablets was 104.40% with RSD below 2%. The accuracy expressed as average recovery of the proposed method was 98.74%, and the precision expressed as relative standard deviation among repeated analysis was 0.55%. The method has adequate sensitivity with detection and quantitation limits of 0.25 and 0.81 μg/mL, respectively. Based on the presented results and according to the ICH and AOAC guidelines on validation of analytical methods, the proposed method was considered precise, accurate with adequate sensitivity, and robust in the MAR quantitative analysis. Therefore, the method can be used in the quality control of chewable veterinary tablets containing MAR.

Słowa kluczowe

EN

quinolone; HPLC-UV; assay method; quality control

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2019
• Tom: Vol. 31, no. 4
• Strony: 291--293
• Opis fizyczny: Bibliogr. 31 poz., rys., tab.

Twórcy

autor

Oliveira K. R. W.

• Programa de Pós-Graduação em Farmácia, Faculdade de Ciências Farmacêuticas, Alimentos e Nutrição, Universidade Federal de Mato Grosso do Sul, Brazil

autor

Sversut R. A.

• Programa de Pós-Graduação em Farmácia, Faculdade de Ciências Farmacêuticas, Alimentos e Nutrição, Universidade Federal de Mato Grosso do Sul, Brazil

autor

Singh A. K.

• Departamento de Farmácia, Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, São Paulo, Brazil

autor

Amaral M. S.

• Instituto de Física, Universidade Federal de Mato Grosso do Sul, Brazil

autor

Kassab N. M.

• Programa de Pós-Graduação em Farmácia, Faculdade de Ciências Farmacêuticas, Alimentos e Nutrição, Universidade Federal de Mato Grosso do Sul, Brazil

Bibliografia

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Uwagi

PL

Opracowanie rekordu w ramach umowy 509/P-DUN/2018 ze środków MNiSW przeznaczonych na działalność upowszechniającą naukę (2019).