Laccase concentration by foam fractionation of Cerrena unicolor and Pleurotus sapidus culture supernatants

• Autorzy: Blatkiewicz M. , Antecka A. , Górak A. , Ledakowicz S.

Identyfikatory

DOI

10.1515/cpe-2017-0035

• Języki publikacji: EN

Abstrakty

EN

Foam fractionation process for concentration of laccases from two Basidiomycete strains under different process conditions was investigated. Culture supernatants of Cerrena unicolor and Pleurotus sapidus containing active laccase were used with and without surfactant additives. Two surfactants: cationic cetrimonium bromide (CTAB) and non-ionic Polysorbate 80 were applied in the range from 0.2 mM to 1.5 mM. The pH levels ranging from 3 to 10 were examined with particular attention to pH=4, which is close to the pI of the enzymes. Results show that the source of the enzyme is significant in terms of partitioning efficiency in a foam fractionation process. Laccase from Cerrena unicolor showed the best activity partitioning coefficients between foamate and retentate of almost 200 with yields reaching 50% for pH 7.5 and concentration of CTAB cCTAB = 0.5 mM, whereas laccase from Pleurotus sapidus showed partitioning coefficients of up to 8 with 25% yield for pH 4 and cCTAB = 0.5 mM.

Słowa kluczowe

EN

foam fractionation; Laccase; CTAB; Polysorbate 80

PL

frakcjonowanie pianą; CTAB; Polisorbat 80

• Wydawca: Komitet Inżynierii Chemicznej i Procesowej Polskiej Akademii Nauk
• Czasopismo: Chemical and Process Engineering
• Rocznik: 2017
• Tom: Vol. 38, nr 3
• Strony: 455--464
• Opis fizyczny: Bibliogr. 31 poz., rys.

Twórcy

autor

Blatkiewicz M.

• Lodz University of Technology, Faculty of Process and Environmental Engineering, ul. Wólczańska 213, 90-942, Łódź, Poland

autor

Antecka A.

• Lodz University of Technology, Faculty of Process and Environmental Engineering, ul. Wólczańska 213, 90-942, Łódź, Poland

autor

Górak A.

• Lodz University of Technology, Faculty of Process and Environmental Engineering, ul. Wólczańska 213, 90-942, Łódź, Poland
• Dortmund University of Technology, The Department of Biochemical and Chemical Engineering, Emil-Figge-Straße 70, D-44227 Dortmund, Germany

autor

Ledakowicz S.

• Lodz University of Technology, Faculty of Process and Environmental Engineering, ul. Wólczańska 213, 90-942, Łódź, Poland

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Uwagi

PL

Opracowanie ze środków MNiSW w ramach umowy 812/P-DUN/2016 na działalność upowszechniającą naukę (zadania 2017)