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Liquid chromatography tandem mass spectrometry (LC-MS/MS) method for the determination of Vildagliptin in rat plasma

Identyfikatory
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
A new sensitive and validated liquid chromatography electro spray ion-tandem mass spectrometry (HPLC-ESI-MS/MS) method for the quantification of Vildagliptin (VG) in rat plasma has been developed and validated using repaglinide (RG) as an internal standard (IS). The analytes were extracted by liquid-liquid extraction using ethyl acetate. Elution of the VG and IS was achieved on a reverse phase Betasil (C18 50 mm 4.6 mm ID, 5 μ) column with an isocratic mobile phase composed of acetonitrile: 2 mM ammonium acetate (90:10 v/v). The analytes monitored in the Multiple Reaction Monitoring (MRM) mode were m/z 304.2→154.0 and 453.3→230.3 for VG and RG, respectively. The calibration curve was linear in the range of 1.57–501.21 ng/mL for VG with lower limit of quantification 1.57 ng/mL. The intra run and inter run precision values are within 11.70% for VG at LOQ level.
Słowa kluczowe
Rocznik
Strony
295--307
Opis fizyczny
Bibliogr. 12 poz., rys., tab.
Twórcy
  • MIDC Industrial Area Alkem Laboratories Ltd, C-17/7 Taloja 410208 Maharashtra India
autor
  • Hanseo University Department of Chemical Engineering 360 Daegok-ri, Haemi-myun, Seoson 360706 Chungcheongnam-do South Korea
  • Dr Reddy’s Laboratories Ltd Bachupalli, Hyderabad 500 072 India
autor
  • Nandha College of Pharmacy Koorapalayam Pirivu, Erode 638052 Tamil Nadu India
autor
  • Hanseo University Department of Chemical Engineering 360 Daegok-ri, Haemi-myun, Seoson 360706 Chungcheongnam-do South Korea
Bibliografia
  • [1] R. Mentlein. Regul. Pept., 85, 9 (1999)
  • [2] A.M. Lambeir, C. Durinx, S. Scharpé, I. De Meester, Crit. Rev. Clin. Lab. Sci.,40, 209 (2003)
  • [3] C.F. Deacon, Horm. Metab. Res., 36, 761 (2004)
  • [4] B. Ahren, Curr. Mol. Med., 13, 154 (2006)
  • [5] I. Vardarli, M.A. Nauck, L.D. Kothe, C.F. Deacon, J.J. Holst, A. Schweizer, J.E. Foley, J. Clin. Endocrin. Metab., 96, 945 (2011)
  • [6] R.I. El-Bagary, E.F. Elkady, B.M. Ayoub, Int. J. Biomed. Sci., 7, 55 (2011)
  • [7] R.I. El-Bagary, E.F. Elkady, B. M. Ayoub, Int. J. Biomed. Sci., 7, 201 (2011)
  • [8] B. Santhosha, A. Ravindranath, Ch. Sundari, Int. Res J Pharm. App. Sci.,2, 22 (2012)
  • [9] A.B. Pharne, B. Santhakumari, A.S. Ghemud, H.K. Jain, M.J. Kulkarni, Int. J. Pharm. Pharm. Sci., 4, 119 (2012)
  • [10] A.T. Barden, B. Salamon, E.E.S. Schapoval, M. Steppe, J. Chromatogr. Sci., 50, 426 (2012)
  • [11] C. Hess, F. Musshoff, B. Madea, Anal. Bioanal. Chem., 400, 33 (2011)
  • [12] Guidance for industry: bioanalytical metod validation, U.S. Department of health and human services, Food and Drug ad mini stration, Centre for Drug Evaluation and Research (CDER) Centre for Veterinary Medicine (CVM), May 2001
Typ dokumentu
Bibliografia
Identyfikator YADDA
bwmeta1.element.baztech-0232c612-0eae-42ba-a3c2-64ef9ef779d5
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