Liquid chromatography tandem mass spectrometry (LC-MS/MS) method for the determination of Vildagliptin in rat plasma

• Autorzy: Sakthimanigandan K. , Ganesh M. , Kanthikiran V. , Sivakumar T. , Jang H.
• Języki publikacji: EN

Abstrakty

EN

A new sensitive and validated liquid chromatography electro spray ion-tandem mass spectrometry (HPLC-ESI-MS/MS) method for the quantification of Vildagliptin (VG) in rat plasma has been developed and validated using repaglinide (RG) as an internal standard (IS). The analytes were extracted by liquid-liquid extraction using ethyl acetate. Elution of the VG and IS was achieved on a reverse phase Betasil (C18 50 mm 4.6 mm ID, 5 μ) column with an isocratic mobile phase composed of acetonitrile: 2 mM ammonium acetate (90:10 v/v). The analytes monitored in the Multiple Reaction Monitoring (MRM) mode were m/z 304.2→154.0 and 453.3→230.3 for VG and RG, respectively. The calibration curve was linear in the range of 1.57–501.21 ng/mL for VG with lower limit of quantification 1.57 ng/mL. The intra run and inter run precision values are within 11.70% for VG at LOQ level.

Słowa kluczowe

EN

Vildagliptin; LC-MS/MS; rat plasma; MRM

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2015
• Tom: Vol. 27, no. 2
• Strony: 295--307
• Opis fizyczny: Bibliogr. 12 poz., rys., tab.

Twórcy

autor

Sakthimanigandan K.

• MIDC Industrial Area Alkem Laboratories Ltd, C-17/7 Taloja 410208 Maharashtra India

autor

Ganesh M.

• Hanseo University Department of Chemical Engineering 360 Daegok-ri, Haemi-myun, Seoson 360706 Chungcheongnam-do South Korea

autor

Kanthikiran V.

• Dr Reddy’s Laboratories Ltd Bachupalli, Hyderabad 500 072 India

autor

Sivakumar T.

• Nandha College of Pharmacy Koorapalayam Pirivu, Erode 638052 Tamil Nadu India

autor

Jang H.

• Hanseo University Department of Chemical Engineering 360 Daegok-ri, Haemi-myun, Seoson 360706 Chungcheongnam-do South Korea

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