Micro-2D-TLC separation of phenolics in some species of mint and their fingerprints on diol bonded polar stationary phase

• Autorzy: Hawrył M. A. , Niemiec M. , Słomka K. , Waksmundzka-Hajnos M. , Szymczak G.
• Języki publikacji: EN

Abstrakty

EN

Micro thin- layer chromatography in two dimensional (2D-mTLC) mode in normal and reversed phase systems by use of diol bonded stationary phase was applied to make fingerprints of 11 species of Mentha genus and two finished pharmaceutical products. Nonaqueous eluents (propan-2-ol or ethyl acetate dissolved in n-heptane) were used in normal phase systems. Mixtures of acetonitrile with water were used in reversed phase chromatographic systems. Optimization of one dimensional systems was performed by determining of RF vs. composition of mobile phases dependencies for standards occurring in various species of Mentha. Most selective eluents were chosen to optimize two-dimensional systems by creating RF in normal-phase (NP) systems vs. RF in reversed-phase (RP) systems correlations. 2D-mTLC on diol polar bonded stationary phase were optimized to separate phenolic compounds and make fingerprints of examined plant materials and this method was never applied earlier in the chromatographic analysis.

Słowa kluczowe

EN

two-dimensional separations; diol bonded polar stationary phase; microthin-layer chromatography; fingerprints; Mentha sp; phenolic compounds

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2016
• Tom: Vol. 28, no. 1
• Strony: 119--127
• Opis fizyczny: Bibliogr. 8 poz., rys., tab.

Twórcy

autor

Hawrył M. A.

• Department of Inorganic Chemistry, Medical University of Lublin, Chodźki 4A St., 20-093 Lublin, Poland

autor

Niemiec M.

• Department of Inorganic Chemistry, Medical University of Lublin, Chodźki 4A St., 20-093 Lublin, Poland

autor

Słomka K.

• Department of Inorganic Chemistry, Medical University of Lublin, Chodźki 4A St., 20-093 Lublin, Poland

autor

Waksmundzka-Hajnos M.

• Department of Inorganic Chemistry, Medical University of Lublin, Chodźki 4A St., 20-093 Lublin, Poland

autor

Szymczak G.

• Botanical Garden of Maria Curie-Skłodowska University in Lublin, Sławinkowska 3, 20-810 Lublin, Poland

Bibliografia

• [1] D.N. Olennikov and L.M. Tankhaeva, Chem. Nat. Comp., 46 (1), 23–27 (2010)
• [2] B. Shan, Y.Z. Cai, M. Sun, and H. Corhe, J. Agric. Food Chem., 53, 7749–7759 (2005)
• [3] M. Gulluce, F. Sahin, M. Sokmen, H. Ozer, D. Daferera, A. Sokmen, M. Polissiou, A. Adiguzel, and H. Ozkan, Food Chem., 103, 1449–1456 (2007)
• [4] M.S. Alia, M. Saleema, W. Ahmada, M. Parvezb, and R. Yamdagnib, Phytochemistry, 59, 889–895 (2002)
• [5] M.A. Hawryl, M.A. Niemiec, and M. Waksmundzka-Hajnos, J. Planar Chromatogr. 26, 141–146 (2013)
• [6] P.K. Zarzycki, J Chromatogr. A 1187, 250–259 (2008)
• [7] P.K. Zarzycki, M.M. Slaczka, E. Wlodarczyk, and M.J. Baran, Chromatographia 76, 1249–1259 (2013)
• [8] M. B. Zarzycka, Camera Separatoria, 3, 129–145 (2011)

Uwagi

PL

Opracowanie ze środków MNiSW w ramach umowy 812/P-DUN/2016 na działalność upowszechniającą naukę.