Monitoring vital functions of A-375 melanoma cell cultures via thin-film nickel capacitors

• Autorzy: Wilczyńska Aleksandra , Kociubiński Andrzej , Zarzeczny Dawid , Szypulski Maciej , Pigoń Dominika , Małecka-Massalska Teresa , Prendecka-Wróbel Monika

Identyfikatory

DOI

10.34821/eng.biomat.157.2020.10-14

• Języki publikacji: EN

Abstrakty

EN

This article deals in the constantly developing branch of microelectronic devices used in various fields of medicine, i.e. diagnostics and treatment of previously incurable human diseases. A method for assessing and monitoring the vital functions of living cells by measuring cellular impedance in real-time using the ECIS® system and a commercial culture substrate is presented. The goal was to develop a substrate significantly less expensive than a commercial substrate that would be suitable for multiple uses and compatible with the ECIS® measurement station. Moreover, thanks to the use of a material with electrochemical properties other than the biocompatible material (gold or platinum) it is possible to observe the cells behavior with regard to the toxic agent. For this purpose, a culture substrate with nickel comb capacitors was used. To make the electrodes, a thin metal layer was sputtered on polycarbonate plates in the magnetron sputtering process. Prior to the next stages, technological masks were designed so as to fit in the ECIS® measuring station. Subsequently, the microelectronic processes of photolithography and etching the metal layer were performed. Finally, the wells were glued onto the culture medium with a biocompatible adhesive. The completed substrates were transferred to the Department of Human Physiology, Medical University of Lublin, for the culture test on A-375 human melanoma cells. The results of the experiment determined the usefulness of the device for monitoring cell culture vital functions by means of impedance measurement.

Słowa kluczowe

EN

ECIS; nickel; thin layers; melanoma; magnetron sputtering

• Wydawca: Polish Society for Biomaterials in Cracow
• Czasopismo: Engineering of Biomaterials
• Rocznik: 2020
• Tom: Vol. 23, no. 157
• Strony: 10--14
• Opis fizyczny: Biblogr. 21 poz., rys., zdj.

Twórcy

autor

Wilczyńska Aleksandra

• Lublin University of Technology, Nadbystrzycka 38A, 20-618 Lublin, Poland

autor

Kociubiński Andrzej

• Lublin University of Technology, Nadbystrzycka 38A, 20-618 Lublin, Poland

autor

Zarzeczny Dawid

• Lublin University of Technology, Nadbystrzycka 38A, 20-618 Lublin, Poland

autor

Szypulski Maciej

• Lublin University of Technology, Nadbystrzycka 38A, 20-618 Lublin, Poland

autor

Pigoń Dominika

• Medical University in Lublin, Radziwiłłowska 11, 20-080 Lublin, Poland

autor

Małecka-Massalska Teresa

• Medical University in Lublin, Radziwiłłowska 11, 20-080 Lublin, Poland

autor

Prendecka-Wróbel Monika

• Medical University in Lublin, Radziwiłłowska 11, 20-080 Lublin, Poland

Bibliografia

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Uwagi

Opracowanie rekordu ze środków MNiSW, umowa Nr 461252 w ramach programu "Społeczna odpowiedzialność nauki" - moduł: Popularyzacja nauki i promocja sportu (2021).