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Simultaneous quantification of cortisol and cortisone in serums and saliva from depressive patients by supported liquid extraction coupled to HPLC–MS/MSs

Identyfikatory
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
Cortisol and cortisone are 2 important glucocorticoids produced in the human hypothalamus–pituitary–adrenal (HPA) axis that respond to stress. An analytical method to determinate cortisol and cortisone in serum and saliva using high-performance liquid chromatography–tandem mass spectrometry following a supported liquid extraction (SLE) was developed. Serum and saliva samples of 0.2 mL were extracted by SLE three times using 0.4 mL of methyl tert-butyl ether each time. The chromatographic separation was obtained on an Agilent Poroshell column using a 0.01% formic acid buffer and acetonitrile (60:40, v/v) as the solvent with a flow rate of 0.3 mL/min. Optimized quantitative mass transitions for cortisol, cortisone, and cortisone d-4 were 363.2/121.0 (m/z), 361.2/163.1 (m/z), and 367.1/270.7 (m/z), respectively. The method validation was achieved according to regulatory guidance. The lower limit of quantification (LLOQ) in serum were 2 ng/mL for cortisol and 1 ng/mL for cortisone, and the LLOQ in saliva were 0.1 ng/mL for cortisol and 0.2 ng/mL for cortisone. The developed method showed convenient and efficient extraction, a lower LLOQ, and a short running time. Modest correlations between serum and saliva cortisol and cortisone concentrations were found. The method was successfully applied in assessing the HPA condition of patients with depressive disorders.
Słowa kluczowe
Rocznik
Strony
269--275
Opis fizyczny
Bibliogr. 23 poz., rys.
Twórcy
autor
  • Xiamen Medical College affiliated Xiamen Xianyue Hospital, 387 Xianyue Road, Xiamen 361012, China
autor
  • Xiamen Medical College affiliated Xiamen Xianyue Hospital, 387 Xianyue Road, Xiamen 361012, China
autor
  • Xiamen Medical College affiliated Xiamen Xianyue Hospital, 387 Xianyue Road, Xiamen 361012, China
autor
  • Xiamen Medical College, 1999 Guankou Middle Road, Xiamen 361023, China
Bibliografia
  • [1] Blair, J.; Adaway, J.; Keevil, B.; Ross, R. Curr. Opin. Endocrinol., Diabetes Obes. 2017, 24, 161.
  • [2] Zankert, S.; Bellingrath, S.; Wust, S.; Kudielka, B. M. Psychoneuroendocrinology 2019, 105, 86.
  • [3] Lewitzka, U.; Bauer, M.; Ripke, B.; Bronisch, T.; Günther, L. Neuropsychobiology 2017, 75, 162.
  • [4] O'Connor, D. B.; Green, J. A.; Ferguson, E.; O'Carroll, R. E.; O'Connor, R. C. Psychoneuroendocrinology 2017, 75, 183.
  • [5] Papadopoulou, A.; Douzenis, A.; Christodoulou, C.; Gournellis, R.; Papageorgiou, C.; Markianos, M. Neuropsychobiology 2017, 76, 161.
  • [6] Islam, M. R.; Islam, M. R.; Ahmed, I.; Moktadir, A. A.; Nahar, Z.; Islam, M. S.; Shahid, S. F. B.; Islam, S. N.; Islam, M. S.; Hasnat, A. SAGE Open Med. 2018, 6, 2050312118773953. Doi: .
  • [7] Williams, S. G.; Turner-Henson, A.; Davis, S.; Soistmann, H. C. Biol. Res. Nurs. 2017, 19, 65.
  • [8] Isaksson, J.; Hogberg, U.; Valladares, E.; Lindblad, F. Psychiatry Res. 2016, 240, 376.
  • [9] Peng, R.; Li, Y. Compr. Psychiatry 2017, 76, 113–118. Doi: .
  • [10] El-Farhan, N.; Rees, D. A.; Evans, C. Ann. Clin. Biochem. 2017, 54, 308.
  • [11] Vieira, J. G. H.; Nakamura, O. H.; Carvalho, V. M. Arq. Bras. Endocrinol. Metabol. 2014, 58, 844.
  • [12] Montsko, G.; Tarjanyi, Z.; Mezosi, E.; Kovacs, G. L. Anal. Bioanal. Chem. 2014, 406, 2333.
  • [13] Fragala, M. S.; Goldman, S. M.; Goldman, M. M.; Bi, C.; Colletti, J. D.; Arent, S. M.; Walker, A. J.; Clarke, N. J. J. Strength Cond. Res. 2018, 32, 2425.
  • [14] Huayllas, M. K. P.; Netzel, B. C.; Singh, R. J.; Kater, C. E. Lab Med. 2018, 49, 259.
  • [15] Kosicka, K.; Siemiatkowska, A.; Szpera-Gozdziewicz, A.; Krzyscin, M.; Breborowicz, G.; Glowka, F. Ann. Clin. Biochem. 2018, 4563218783789.
  • [16] Abujaber, F.; Corps Ricardo, A. I.; Rios, A.; Guzman Bernardo, F. J.; Rodriguez Martin-Doimeadios, R. C. J. Pharm. Biomed. Anal. 2019, 165, 141.
  • [17] Peti, A. P. F.; Locachevic, G. A.; Prado, M. K. B.; Moraes, L. A. B.; Faccioli, L. H. J. Mass Spectrom. 2018, 53, 423.
  • [18] Tamashima, E.; Kai, T.; Todoroki, K.; Kawami, Y.; Itoyama, M.; Hayama, T.; Yoshida, H.; Yamaguchi, M.; Nohta, H. Bunseki Kagaku 2013, 62, 719.
  • [19] Sauve, E. N.; Langodegard, M.; Ekeberg, D.; Oiestad, A. M. J. Chromatogr. B: Anal. Technol. Biomed. Life Sci. 2012, 883–884, 177.
  • [20] Gangu Naidu, C.; Nageswara Rao, R.; Prasada Rao, A. V.; Nagesh Kumar, K.; Padiya, R.; Madhusudhan Rao, V. J. Chromatogr. Sci. 2018, 56, 879.
  • [21] Ding, X.; Li, F.; McKnight, J.; Schmidt, C.; Strooisma, K.; Shimizu, H.; Faber, K.; Ware, J. A.; Dean, B. J. Pharm. Biomed. Anal. 2014, 100, 150.
  • [22] Turpeinen, U.; Hämäläinen, E. Best Pract. Res., Clin. Endocrinol. Metab. 2013, 27, 795.
  • [23] Debono, M.; Harrison, R. F.; Whitaker, M. J.; Eckland, D.; Arlt, W.; Keevil, B. G.; Ross, J. C. J. Clin. Endocrinol. Metab. 2016, 101, 1469.
Uwagi
PL
Opracowanie rekordu ze środków MNiSW, umowa Nr 461252 w ramach programu "Społeczna odpowiedzialność nauki" - moduł: Popularyzacja nauki i promocja sportu (2020).
Typ dokumentu
Bibliografia
Identyfikator YADDA
bwmeta1.element.baztech-008fa436-82f0-4763-ac63-a734ab37a44d
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