Simultaneous quantification of cortisol and cortisone in serums and saliva from depressive patients by supported liquid extraction coupled to HPLC–MS/MSs

• Autorzy: Chen Binbin , Lyu Haiyan , Xu Xiangzhen , Wang Chen

Identyfikatory

DOI

10.1556/1326.2020.00733

• Języki publikacji: EN

Abstrakty

EN

Cortisol and cortisone are 2 important glucocorticoids produced in the human hypothalamus–pituitary–adrenal (HPA) axis that respond to stress. An analytical method to determinate cortisol and cortisone in serum and saliva using high-performance liquid chromatography–tandem mass spectrometry following a supported liquid extraction (SLE) was developed. Serum and saliva samples of 0.2 mL were extracted by SLE three times using 0.4 mL of methyl tert-butyl ether each time. The chromatographic separation was obtained on an Agilent Poroshell column using a 0.01% formic acid buffer and acetonitrile (60:40, v/v) as the solvent with a flow rate of 0.3 mL/min. Optimized quantitative mass transitions for cortisol, cortisone, and cortisone d-4 were 363.2/121.0 (m/z), 361.2/163.1 (m/z), and 367.1/270.7 (m/z), respectively. The method validation was achieved according to regulatory guidance. The lower limit of quantification (LLOQ) in serum were 2 ng/mL for cortisol and 1 ng/mL for cortisone, and the LLOQ in saliva were 0.1 ng/mL for cortisol and 0.2 ng/mL for cortisone. The developed method showed convenient and efficient extraction, a lower LLOQ, and a short running time. Modest correlations between serum and saliva cortisol and cortisone concentrations were found. The method was successfully applied in assessing the HPA condition of patients with depressive disorders.

Słowa kluczowe

EN

cortisol; cortisone; LC-MS/MS; supported liquid extraction

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2020
• Tom: Vol. 32, no. 4
• Strony: 269--275
• Opis fizyczny: Bibliogr. 23 poz., rys.

Twórcy

autor

Chen Binbin

• Xiamen Medical College affiliated Xiamen Xianyue Hospital, 387 Xianyue Road, Xiamen 361012, China

autor

Lyu Haiyan

• Xiamen Medical College affiliated Xiamen Xianyue Hospital, 387 Xianyue Road, Xiamen 361012, China

autor

Xu Xiangzhen

• Xiamen Medical College affiliated Xiamen Xianyue Hospital, 387 Xianyue Road, Xiamen 361012, China

autor

Wang Chen

• Xiamen Medical College, 1999 Guankou Middle Road, Xiamen 361023, China

Bibliografia

• [1] Blair, J.; Adaway, J.; Keevil, B.; Ross, R. Curr. Opin. Endocrinol., Diabetes Obes. 2017, 24, 161.
• [2] Zankert, S.; Bellingrath, S.; Wust, S.; Kudielka, B. M. Psychoneuroendocrinology 2019, 105, 86.
• [3] Lewitzka, U.; Bauer, M.; Ripke, B.; Bronisch, T.; Günther, L. Neuropsychobiology 2017, 75, 162.
• [4] O'Connor, D. B.; Green, J. A.; Ferguson, E.; O'Carroll, R. E.; O'Connor, R. C. Psychoneuroendocrinology 2017, 75, 183.
• [5] Papadopoulou, A.; Douzenis, A.; Christodoulou, C.; Gournellis, R.; Papageorgiou, C.; Markianos, M. Neuropsychobiology 2017, 76, 161.
• [6] Islam, M. R.; Islam, M. R.; Ahmed, I.; Moktadir, A. A.; Nahar, Z.; Islam, M. S.; Shahid, S. F. B.; Islam, S. N.; Islam, M. S.; Hasnat, A. SAGE Open Med. 2018, 6, 2050312118773953. Doi: .
• [7] Williams, S. G.; Turner-Henson, A.; Davis, S.; Soistmann, H. C. Biol. Res. Nurs. 2017, 19, 65.
• [8] Isaksson, J.; Hogberg, U.; Valladares, E.; Lindblad, F. Psychiatry Res. 2016, 240, 376.
• [9] Peng, R.; Li, Y. Compr. Psychiatry 2017, 76, 113–118. Doi: .
• [10] El-Farhan, N.; Rees, D. A.; Evans, C. Ann. Clin. Biochem. 2017, 54, 308.
• [11] Vieira, J. G. H.; Nakamura, O. H.; Carvalho, V. M. Arq. Bras. Endocrinol. Metabol. 2014, 58, 844.
• [12] Montsko, G.; Tarjanyi, Z.; Mezosi, E.; Kovacs, G. L. Anal. Bioanal. Chem. 2014, 406, 2333.
• [13] Fragala, M. S.; Goldman, S. M.; Goldman, M. M.; Bi, C.; Colletti, J. D.; Arent, S. M.; Walker, A. J.; Clarke, N. J. J. Strength Cond. Res. 2018, 32, 2425.
• [14] Huayllas, M. K. P.; Netzel, B. C.; Singh, R. J.; Kater, C. E. Lab Med. 2018, 49, 259.
• [15] Kosicka, K.; Siemiatkowska, A.; Szpera-Gozdziewicz, A.; Krzyscin, M.; Breborowicz, G.; Glowka, F. Ann. Clin. Biochem. 2018, 4563218783789.
• [16] Abujaber, F.; Corps Ricardo, A. I.; Rios, A.; Guzman Bernardo, F. J.; Rodriguez Martin-Doimeadios, R. C. J. Pharm. Biomed. Anal. 2019, 165, 141.
• [17] Peti, A. P. F.; Locachevic, G. A.; Prado, M. K. B.; Moraes, L. A. B.; Faccioli, L. H. J. Mass Spectrom. 2018, 53, 423.
• [18] Tamashima, E.; Kai, T.; Todoroki, K.; Kawami, Y.; Itoyama, M.; Hayama, T.; Yoshida, H.; Yamaguchi, M.; Nohta, H. Bunseki Kagaku 2013, 62, 719.
• [19] Sauve, E. N.; Langodegard, M.; Ekeberg, D.; Oiestad, A. M. J. Chromatogr. B: Anal. Technol. Biomed. Life Sci. 2012, 883–884, 177.
• [20] Gangu Naidu, C.; Nageswara Rao, R.; Prasada Rao, A. V.; Nagesh Kumar, K.; Padiya, R.; Madhusudhan Rao, V. J. Chromatogr. Sci. 2018, 56, 879.
• [21] Ding, X.; Li, F.; McKnight, J.; Schmidt, C.; Strooisma, K.; Shimizu, H.; Faber, K.; Ware, J. A.; Dean, B. J. Pharm. Biomed. Anal. 2014, 100, 150.
• [22] Turpeinen, U.; Hämäläinen, E. Best Pract. Res., Clin. Endocrinol. Metab. 2013, 27, 795.
• [23] Debono, M.; Harrison, R. F.; Whitaker, M. J.; Eckland, D.; Arlt, W.; Keevil, B. G.; Ross, J. C. J. Clin. Endocrinol. Metab. 2016, 101, 1469.

Uwagi

PL

Opracowanie rekordu ze środków MNiSW, umowa Nr 461252 w ramach programu "Społeczna odpowiedzialność nauki" - moduł: Popularyzacja nauki i promocja sportu (2020).