BazTech - Yadda

1

Fibrous structures based of natural polymers for tissue engineering applications

Stodolak-Zych E., Ścisłowska-Czarnecka A., Kolesinska B., Cieślak M., Puchowicz D., Kaminska I., Bogun M.

Engineering of Biomaterials

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2018

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Vol. 21, no. 148

63

2

The membrane with polylactide and hyaluronic fibers for skin substitute

Stodolak-Zych E., Rozmus K., Dzierzkowska E., Zych Ł., Kapacz-Kmita A., Gargas M., Kołaczkowska E., Cieniawska M., Książek A., Ścisłowska-Czarnecka A.

Acta of Bioengineering and Biomechanics

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2018

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Vol. 20, no. 4

91--99

EN

Skin substitutes are heterogeneous group of scaffolds (natural or synthetic) and cells. We hypothesize that nanofibers with layer composition made of polylactide (PLA) and sodium hyaluronate (HA) obtained using electrospinning method are a good matrix for cell adhesion and proliferation. Methods: Optimal conditions of electrospinning of PLA and HA nanofibers to create layered compositions (PLA membrane covered with HA nonwovens) were determined by modifying parameters such as the appropriate amount of solvents, polymer concentration, mixing temperature and electrospinning process conditions. By changing the parameters, it was possible to control the diameter and properties of both polymer fibers. The spinning solution were characterized by surface tension and rheology. A scanning electron microscope (SEM) was used to determine the morphology and fiber diameters: PLA and HA. Structure of the PLA/HA nonwoven was analyzed using spectroscopy (FTIR/ATR). Biocompatibility of the nonwoven with fibroblasts (ECM producers) was assessed in the in vitro conditions. Results: The results showed that stable conditions for the formation of submicron PLA fibers were obtained using a 13% wt. solution of the polymer, dissolved in a 3:1 mixture of DCM:DMF at 45 °C. The hyaluronic fibers were prepared from a 12% wt. solution of the polymer dissolved in a 2:1 mixture of ammonia water and ethyl alcohol. All materials were biocompatible but to a different degree. Conclusions: The proposed laminate scaffold was characterized by a hydrophobic-hydrophilic domain surface with a maintained fiber size of both layers. The material positively underwent biocompatibility testing in contact with fibroblasts.

3

Evaluation of cytocompatibility of PLGA and PGLA-based nanocomposite biomaterials in osteoblast cultures

Ścisłowska-Czarnecka A., Stodolak-Zych E., Prawdzik E., Boguń M., Menaszek E.

Engineering of Biomaterials

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2012

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Vol. 15, no. 112

2-5

EN

The aim of this study was to evaluate biocompatibility of multilevel composites based on bioresorbable poly(lactide-co-glycolide) (PGLA). Polymer matrix was modified with multidimensional (MD) short biopolymer fibers of calcium alginate (CA) containing bioactive ceramic nanoparticles (nanohydroxyapatite - HA or nanosilica - SiO2). The nanocomposite fibres present in the polymer samples influenced cells morphology, viability and secretory activity which was estimated using human osteoblasts cells (NHOst). The results indicate that biodegradable nanocomposite CA-HA/PGLA improves biological properties of the basic biomaterial (PGLA) suggesting its potential application for bone tissue engineering.

4

Biocomatibility study of BOC polymer mesh enriched with HAp and TCP covered by PCL fibres

Menaszek E., Ścisłowska-Czarnecka A., Draczyński Z., Bogun M., Stodolak-Zych E.

Engineering of Biomaterials

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2012

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Vol. 15, no. 116-117 spec. iss.

151--152

EN

The study was conducted in order to determine the biocomatibility of polimer mesh based on BOC and enriched with HAp or TCP coverd by PCL sub-micrometric fibres. Human osteoblast cell line NHOst was cultured in standard conditions on disk-shaped polymer samples. Interactions between materials and cells were examined through microscopic observation of cells' adhesion and morphology, and tests of viability/proliferation and cytotoxicity. The study proved the biocompatibility of all examined materials, though the surface of TCP enriched polymer didn't promote the adhesion of cells.

5

Composites Based on Poly-e-Caprolactone and Calcium Alginate Fibres Containing Ceramic Nanoadditives for Use in Regenerative Medicine

Boguń M., Stodolak E., Menaszek E., Ścisłowska-Czarnecka A.

Fibres & Textiles in Eastern Europe

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2011

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Vol. 19, no. 6 (89)

17—21

EN

The aim of the present work was to develop new composite materials based on two biocompatible polymers (sodium alginate and polycaprolactone) intended for use in the treatment of bone tissue defects. Tests carried out to obtain polymer-fibre composites using two resorbable polymers demonstrated the possibility of attaining composites with mechanical properties that are suitable from the point of view of their applications. Young’s modulus values for the composite systems analysed (254-389 MPa) are higher than for an unmodified PCL sheet. Irrespective of the fibrous phase used, the PCL matrix demonstrates stability in in vitro conditions. The constant pH values and small changes in the ionic conductance of the water indicate that these materials undergo gradual but slow degradation.

PL

W pracy opracowano warunki wytwarzania kompozytów opartych o dwa biodegradowalne tworzywa. Przeprowadzone badania wykazały zróżnicowany wpływ obecności poszczególnych rodzajów fazy włóknistej na właściwości wytrzymałościowe, właściwości fizykochemiczne powierzchni oraz stabilność materiałów kompozytowych podczas procesu inkubacji w warunkach in vitro. W oparciu o uzyskane wyniki można przypuszczać, iż wytworzone materiały kompozytowe na bazie włókien alginianowych, będących nośnikami substancji czynnych, będą powodować wytworzenie dogodnych warunków do krystalizacji apatytu w żywym organizmie.

6

Fibroblast biological activity on poly(l-lactide) and poly(l-lactide-co-trimethylene carbonate)

Ścisłowska-Czarnecka A., Pamuła E., Kołaczkowska E.

Engineering of Biomaterials

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2011

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Vol. 14, no. 102

7--10

EN

Poly-L-lactide (PLLA) is acknowledged biocompatible polyester. However, it possesses high crystallinity/brittleness/stiffness and requires long time for complete degradation. In the current study we present data on PLTMC, a copolymer of L-lactide and trimethylene carbonate (TMC). Poly(trimethylene carbonate) (PTMC) is characterised by good mechanical properties and rapid degradation rate and for this it might possess new desired features for medical applications. During the experiments, adhesion and activity of fibroblasts cultured on PLLA and PLTMC were studied and compared during two time points of 3 and 5 days. On day 3, the number of adherent fibroblasts was compromised when fibroblasts were cultured in the presence of PLTMC but the proper adherence was recovered by day 5. The same pat-tern was observed when we evaluated some activity parameters of fibroblasts. In particular, the release of proteins and nitric oxide was studied as the increased levels of the mediators might indicate unwanted inflammatory-like condition. Overall, the results suggest that the synthesized PLTMC initially shows unwanted effects on fibroblasts but with the time these effects are abolished. Therefore PLTMC seems to represent a new material that is non-cytotoxic and compatible with the living cells.

7

Chemical modification of poly ε-caprolactone with wollastonite and its influence on biological properties of osteoblast like-cells MG-63

Ścisłowska-Czarnecka A., Menaszek E., Kołaczkowska E., Błażewicz M., Podporska J.

Engineering of Biomaterials

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2011

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Vol. 14, no. 102

11--14

EN

PCL (poly-ε-caprolactone) is a biocompatible and biodegradable polymer of aliphatic polyester group. However, PCL does not effectively bind to the bone in contrast to bioactive inorganic compounds such as wollastonite. For this wollastonite (WS) is regarded as a potential bioactive material for bone tissue engeenering although its main drawback is brittlennes. Therefore we synthesized polymer nanocomposite materials composed of poly-ε-caprolactone and wollastonite (PCL/wollastonite) containing either 0.5% or 5% of the latter modifying filler. And we aimed to verify biological properties of the nanocomposite PCL/WS materials, in comparison to the pure PCL, on cultures of osteoblast-like cells MG-63. The study revealed that the adherence of the osteoblast-like cells to the tested materials was enhanced by the PCL modification (PCL/5WS > PCL/0.5WS > PCL) while cell viability/proliferation was not altered. Furthermore, the activity of alkaline phosphatase indicative of osteoblast differentiation (maturation) was enhanced when the cells were cultured with either PCL/5WS or PCL/0.5WS. Overall, our results indicate that PCL-modified wollastonite improves biological properties of the basic biomaterial suggesting its potential usefulness/application for the bone tissue regeneration.

8

Surface characterization, collagen adsorption and cell behaviour on poly(L-lactide-co-glycolide)

Adamczak M., Ścisłowska-Czarnecka A., Genet M. J., Dupont-Gillain C. C., Pamula E.

Acta of Bioengineering and Biomechanics

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2011

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Vol. 13, no. 3

63-75

EN

Poly(L-lactide-co-glycolide) (PLG) was modified through the adsorption of collagen to improve the behaviour of fibroblasts and osteoblasts. As reference materials cell-resistant polystyrene (PS) and cell-conductive tissue-culture polystyrene (TCPS) were also evaluated. The physicochemical surface properties of the materials were studied by X-ray photoelectron spectroscopy (XPS), atomic force microscopy (AFM), and water contact angle measurements. The morphology of cells was examined using optical microscopy, while their growth was evaluated by both crystal violet and MTT tests. Nitric oxide level and protein concentration were tested in cell supernatants. The results showed that the adsorbed amount and the organization of the adsorbed collagen were influenced by surface hydrophobicity. Cell culture experiments on native substrates revealed that cell attachment, spreading and growth enhanced, depending on the substrate, in the following order: PS

9

Enhanced cell integration to titanium-grafit composite

Menaszek E., Ścisłowska-Czarnecka A., Deptuła P., Dąbrowski J. R.

Engineering of Biomaterials

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2010

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Vol. 13, no. 99-101

122

10

Biocompatibility of hybrid fibrous materials basing on poly-L/DL-lactide

Stodolak E., Ścisłowska-Czarnecka A., Błażewicz M., Bogun M., Mikolajczyk T., Menaszek E.

Engineering of Biomaterials

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2010

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Vol. 13, no. 99-101

110

EN

Hybrid biomaterials due to their unique structure may become an alternative for many popular composite and nanocomposite materials. Multilevel modification of their matrix manifesting itself in the presence of particles of different sizes i.e., micrometric, submicrometric and nanometric together with the variety of shapes of a modyfing phase (nanometric fibres, submicron particles, coated nanoparticles) and its different chemical character make the hybrid materials similar to natural tissue. Bone tissue structure is particulary close to this model in which collagen fibres and hydroxyapatite particles and nanoparticles have not only different form but first of all they play different role in the tissue which depends on their chemical nature. In the biomedical engineering syntetic hybride biomaterials are usually produced using resorbable and degradable polymer matrices and inorganic filers (ceramic bioactive particles; HAp, TCP, SiO2) or organic filers (collagen, polysaccharides e.g. alginate fibres). The main function of the modyfing phase is inprovement of the polymer matrix leading to bioactive, stronger material showing high biofunctionality. Production of hybrid materials is based mainly on experimental works, which is related to the presence in their matrix few phases with different properties which may interact. Hybrid materials do not follow the rule of mixtures thus it is difficult to predict behaviour of a material in which co-exis different chemical and phisical phases. In the work hybrid composite foils were produced in which modyfing phase consisted in; nanocomposite calcium alginate fibres modyfied with ceramic nanoparticles; HAp (CAH fibres), TCP (CAT fibres), SiO2 (CAS fibres) and MMT (CAM fibres). Short fibres were subjected to additional size reduction in vibration ball mill resultiong in submicron and nanometric phases. Size of the particels after grinding was determined by screening analysis and DLS method (for particels smaller than 500 nm). It was observed than the population of short fibres consist in three fractions i.e.; micrometric (~2μm, 50 wt.%), submicrometric (500–800 nm, 40 wt.%) and nanometric ( below 500 nm, 10 wt.%). The fibres and products of their grinding were homogenised in P(L/ DL)LA polymer solution (poly-L/DL-lactide, Purarorb 80, Purac Germany). A hybride material in the form of thin foils containing 2 wt.% of a modyfing phase were subjected to durability tests consisting in incubation in distilled water (30 days/37C). Monitoring of the medium pH and conductivity did not show changes related to harmful products of their decomposition. Osteoblast-like cells from MG-63 line contacted with the surface of the materials showed high viability (MMT test) comparable with the reference material (TCPS). High degree of adherence of the cells to the materal surface (CV test) testifies of potential abilities of the material stimulating proliferation of bone tissue cells. The highes rate of dynamic growth (increase of the cells number after 7 days of incubation) was observed for the material which was modified with CAS fibres and products of their grinding. The performed investigations have a preliminary character. Their results testify for potential osteoconductive or osteoinductive abilities of hybride materials basing on P(L/DL)LA and alginate nanocomposite fibres.

11

Porous titanium scaffolds with modified surface: in vitro cell biology assessment

Ścisłowska-Czarnecka A., Menaszek E., Szaraniec B., Chłopek J., Kołaczkowska E.

Engineering of Biomaterials

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2009

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Vol. 12, no. 88

5--10

EN

Interaction of host cells with a biomaterial surface is important for biocompatibility and thus is essential for biomedical applications. Therefore investigations are undertaken to scrutinize for an appropriate surface coating with physical and chemical properties minimizing undesirable activation of immunological response. For this the current study was aimed at examining the effects of different surface modifications of titanium by its coating with ceramic materials - hydroxyapatite, bioglass and CaO-SiO2on osteoblast morphology and secretory activity. Titanium is known for its excellent mechanical properties but its surface has low bioactivity. We report that CaO-SiO2coating decreased a number of attached osteoblasts and altered their morphology. Moreover, the ceramic coatings temporarily upregulated release of pro-inflammatory cytokines IL-6 (all of them) and TNF-α (CaO-SiO2). However, overall the levels of the cytokines were low. In contrast, levels of neutrophil-attracting chemokine IL-8 were the highest. IL-8 was produced mostly by cells incubated with hydroxyapatite titanium coating in contrary to those incubated with either bioglass or CaO-SiO2titanium modifications. In conclusion, the titanium coated with ceramics such as hydroxyapatite or bioglass had the best effect on cell adhesion; however, hydroxyapatite might potentially stimulate destructive neutrophils while CaO-SiO2-coating has a negative effect on cell adhesion.

12

Wpływ biomateriałów na przyleganie i aktywność mysich fibroblastów L929

Ścisłowska-Czarnecka A., Pamuła E., Płytycz B., Kołaczkowska E.

Engineering of Biomaterials

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2008

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Vol. 11, no. 81-84

83--86

13

The effect of titanium alloy modified with a-C:N:H and a-SiCxNY(H) coatings on adhesion and immune response of human osteoblast-like MG-63 cells

Ścisłowska-Czarnecka A., Menaszek E., Kołaczkowska E., Januś M., Stypuła B.

Engineering of Biomaterials

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2008

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Vol. 11, no. 81-84

126--128

EN

The study was conducted in order to determine the effects of modified titanium alloy (Ti-6Al-4V) surfaces on the biological response of a human osteoblast-like cell line. MG-63 cells were cultured on disk-shaped Ti-alloys: unmodified, and covered with a-C:N:H or a-SiCxNy(H) layers. Interactions between materials and cells were examined through determination of cells adhesion and secretion of cytokines involved in the development of immune response.

14

Comparative study of hydroxyapatite and hydroxyapatite mixed with bioglass coatings of metallic implants, deposited by PLD method

Mróz W., Jedyński M., Burdyńska S., Prokopiuk A., Ślósarczyk A., Menaszek E., Ścisłowska-Czarnecka A., Łączka M., Cholewa-Kowalska K., Niedzielska A.

Engineering of Biomaterials

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2008

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Vol. 11, no. 81-84

121--123

EN

Hydroxyapatite and bioglasses are important bioactive materials as they exhibit direct bonding with human bone. Therefore they are used as coatings for metallic implants. The aim of the present study was to compare two types of layers: monophase hydroxyapatite (HA) and composite-type HA+BG hydroxyapatite mixed with bioglass (BG) (BG 50% of weight) during the initial stage of their interaction with cell medium. In vitro studies were performed in order to determine the effect of the investigated layers on cell response. After 3 and 7 days the behaviour of the cells grown on the above surfaces was estimated through determination of the cell adhesion (CV colorimetric assay). Cell morphology and properties of biomaterials surfaces were analysed by atomic force microscopy (AFM).

15

Immobilization of collagen - an effective method of improving cell adhesion on polymeric materials

Pamuła E., Ścisłowska-Czarnecka A.

Engineering of Biomaterials

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2007

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Vol. 10, no. 63-64

20-23

EN

Surface properties of poly(L-lactide-co-glycolide) (PLG), and two reference materials: hydrophobic polystyrene (PS) and hydrophilic tissue culture polystyrene (TCPS) were modified by collagen adsorption. The morphology of the obtained collagen film was observed by using atomic force microscopy. On PLG and TCPS collagen layer was uniform, while on PS collagen formed isolated patches. The differences in supramolecular organization of collagen were due to differences in surface wettability. The behaviour of L929 fibroblasts incubated on all raw and collagen-modified surfaces was then evaluated. The best adhesion and spreading of cells, as expected, were observed on TCPS. Collagen adsorbed on PLG and PS considerably improved adhesion and spreading of fibroblasts.

16

Polisiloksanowe powłoki implantów medycznych : wpływ plazmy fluorowej na skład chemiczny i właściwości polisiloksanu

Szmigiel D., Domański K., Prokaryn P., Grabiec P., Pamuła E., Ścisłowska-Czarnecka A., Płytycz B.

Inżynieria Biomateriałów

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2006

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R. 9, nr 58-60

206-209

17

Modyfikacja chemiczna kopolimeru glikolidu z epsylon-kaprolaktonem i jej wpływ na adhezję i żywotność fibroblastów in vitro

Pamuła E., Ścisłowska-Czarnecka A., Szlęk A., Chadzińska M., Dobrzyński P., Płytycz B.

Inżynieria Biomateriałów

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2006

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R. 9, nr 58-60

24-28

PL

W celu poprawienia biozgodności kopolimeru glikolidu z epsylon-kaprolaktonem (PGCap) został on zmodyfikowany poprzez inkubację przez różne okresy czasu w roztworze wodnym zasady sodowej. Zastosowana modyfikacja nie wpłynęła na chropowatość powierzchni lecz spowodowała wytworzenie tlenowych grup funkcyjnych, które nadały powierzchni charakter polarny i spowodowały obniżenie hydrofobowości. Badania in vitro wykazały lepszą adhezję i żywotność fibroblastów (L929) hodowanych na próbkach modyfikowanych powierzchniowo.

EN

Copolymer of glycolide and epsylon-caprolactone was modified by incubation in aqueous solution of sodium hydroxide for different periods of time in order to improve its biocompatibility. Applied modification did not influence surface roughness but created oxygenated functions, which enhance surface polarity and diminish hydrophobicity. In vitro studies showed better adherence and viability of fibroblasts (L929) cultivated on surface-modified samples.