Study of toxoplasmosis in type 2 diabetic patients using ELISA and B1 nested-PCR methods

• Autorzy: Saki J. , Khodkar I. , Shadnoosh F. , Safi M. , Ghadiri A. , Shafieenia S.
• Języki publikacji: EN

Abstrakty

EN

Toxoplasma gondii (T. gondii) is a ubiquitous, opportunistic organism, which actually infects all warmblooded animals. Diabetes is a silent, irritating metabolic disorder among which type 2 diabetes includes over 90% of cases globally. This case-control study was aimed to detect T. gondii infection in type 2 diabetic patients in Khuzestan province, southwest of Iran. Serological enzyme-linked immunosorbent assay (ELISA) and molecular polymerase chain reaction (PCR) method targeting B1 gene were employed to comparatively detect the parasitic infection among 377 diabetic patients and 200 non-diabetic subjects during 2016–2018. Considerably higher anti-T. gondii antibodies were determined in case group 44.29% (167/377), than in control group 19% (38/200) (P<0.05). Among diabetic patients, 153 (40.58%) were seropositive for IgG and 14 (3.71%) were seropositive for IgM, while 35 (17.5%) and 3 (1.5%) healthy people were seropositive regarding IgG and IgM, respectively (P<0.05). By nested PCR, B1 gene was identified in 36 out of 167 (21.55%) and 5 out of 38 (13.15%) of the seropositive samples of case and control groups, respectively. The prevalence of anti-T. gondii antibodies and DNA in diabetic patients was significantly higher than in non-diabetic patients. Thus, it could be recommended to routinely evaluate the chronicity of the infection in diabetic patients.

• Wydawca: -
• Czasopismo: Annals of Parasitology
• Rocznik: 2022
• Tom: 68
• Numer: 2
• Opis fizyczny: p.367-373,fig.,ref.

Twórcy

autor

Saki J.

• Health Research Institute, Infectious and Tropical Diseases Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran

autor

Khodkar I.

• Department of Medical Parasitology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
• Student Research Committee, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran

autor

Shadnoosh F.

• Department of Medical Parasitology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran

autor

Safi M.

• Department of Medical Parasitology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran

autor

Ghadiri A.

• Department of Immunology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran

autor

Shafieenia S.

• Department of Medical Parasitology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran

Identyfikatory

DOI

10.17420/ap6802.442