Over 18% of the agricultural area of Lithuania is acidic. The reduction of soil liming caused by the present economic situation brings up urgent need to understand the behavior of crops under higher acidity of the soil. The present study is aimed to describe the variations in acid and heavy metal tolerance of the seedlings of spring barley (Hordeum vulgare lo) cultivars grown in two solutions of different acidity (pH 4.5 and 5.6). For evaluation of metal toxicity, cultivars 'Aura', 'Alsa', 'Ula', 'Auksiniai-3', 'Baronesse' and 'Dzivostlij' were selected. Reaction of cultivars to equimolar (592 /lM) aqueous solutions of 3CdSO4 . 8H2O, CUSO4, Pb(NOJh, MnSO4 . 5H2O and AIK(SO4)2 was examined. Significant differences were found between sampies treated with various metals (Cd, Cu, Al, Pb, Mn), as well as between samples treated with metal s and control samples. Differences between samples treated at pH 4.5 and pH 5.6 were insignificant. There was strong correlation (r = 0.862) between dry weight and length of the roots, and between dry weight and length of the shoots (r = 0.891). our study revealed that 'Dzivosnij' and 'Auksiniai 3' were the cultivars most sensitive to metals, while the least sensitive one was' Aura', which will be used for further acidity and metal tolerance assessments.