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1 Polish Journal of Medical Physics And Engineering

1 2008

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Free radicals in a conglomerate of peripheral blood with a spin trap investigated by the EPR method before and after angioplasty treatment

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Krzyminiewski R. , Kruczynski Z. , Stepien A. , Dobosz B.

Polish Journal of Medical Physics And Engineering

2008

tom 14

nr 1

1-12

The Electron Paramagnetic Resonance (EPR) was used to investigate free radicals in the peripheral blood of patients subjected to angioplasty treatment. To detect these free radicals, a nitrosobenzene spin trap was used in this experiment. The EPR spectra of the blood with a spin trap conglomerate was measured at room temperature and at 170 K. To confirm the kind of free radicals in the conglomerate blood-spin trap, simulation and quantum-chemical calculations were made, and the conglomerate spin trap with ascorbic acid was measured. Two different types of radicals, one at room temperature and the other in a frozen sample of blood, were found.