Wyniki wyszukiwania - Biblioteka Nauki

1

The influence of elastin degradation products, glucose and atorvastatin on metalloproteinase-1, -2, -9 and tissue inhibitor of metalloproteinases-1, -2, -3 expression in human retinal pigment epithelial cells

100%

Dorecka M. , Francuz T. , Garczorz W. , Siemianowicz K. , Romaniuk W.

Acta Biochimica Polonica

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2014

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tom 61

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nr 2

265-270

EN

Purpose: Hyperglycemia and increased concentrations of elastin degradation products (EDPs) are common findings in patients with diabetes, atherosclerosis and hypertension. The aim of this study was to assess the influence of high glucose, EDPs and atorvastatin on MMP-1, MMP-2, MMP-9 and TIMP1-3 gene expression in human retinal pigment epithelial cells (HRPE) in vitro. Method: HRPE were cultured for 24 hours with the substances being tested (glucose, EDPs), alone or in combination. Additionally, the cells were treated with atorvastatin in two different concentrations (1 or 10 μM). After incubation, total cellular RNA was extracted and used for gene expression evaluation. Gene expression was measured using the real-time RT-PCR technique. Results: Glucose, EDPs and atorvastatin had no impact on TIMP-1 and TIMP-3 expression. HRPE cells treated with glucose or EDPs with the addition of atorvastatin had a statistically significant decrease of TIMP-2 expression; glucose alone decreased MMP-1 expression. Atorvastatin decreased expression of all assessed genes, except TIMP-1 and TIMP-3 in a dose-dependent manner. Conclusions: Our results confirm the importance of MMPs and TIMPs in retinal vascular biology. Atorvastatin-induced MMPs gene expression can deeply affect extracellular matrix turnover, which may play an important role in the progression of ocular diseases.

2

Expression of the type 1 metalloproteinase in the rat hippocampus after the intracerebroventricular injection of beta-amyloid peptide (25-35)

75%

Ierusalimsky V. N. , Kuleshova E. P. , Balaban P. M.

Acta Neurobiologiae Experimentalis

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2013

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tom 73

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nr 4

EN

The expression of matrix metalloproteinase of the first type was studied in frontal sections of the adult rat brain one month after a single intracerebroventricular injection of P-amyloid peptide (25-35), which is known to be a well-known model of the development of Alzheimer's disease. Brain sections were stained immunocytochemically to detect MMP-1 expression, and histologically to reveal the state of hippocampal neurons. Administration of P-amyloid peptide induced a significant degeneration of cells in the dorsal hippocampus. This was demonstrated by a significant decrease in the total number of cells and by the appearance of acidophilic neurons of altered (often triangular) shape. Altered cells were most often found in the hippocampal field CA3, and in a smaller quantity in the CA1 field. MMP-1-like immunoreactivity was found in the same hippocampal areas, the staining being restricted to the cells of altered shape (staining of somata and primary neurites). The data suggest possible involvement of the type 1 metalloproteinase in the development of Alzheimer's disease.

3

The relationships between the immunoexpression of KAI1, MMP-2, MMP-9 and steroid receptors expression in endometrial cancer

75%

Grybos A. , Bar J.

Folia Histochemica et Cytobiologica

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2014

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tom 52

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nr 3

4

IGF-I and IGF-binding proteins in synovial fluid of patients with Lyme arthritis

75%

Bączyk J. , Guszczyn T. , Romanowicz L. , Pancewicz S. , Moniuszko A. , Popko J.

Progress in Health Sciences

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2016

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tom 6(1)

49-56

EN

Purpose: Multiple cellular functions are stimulated by a Insulin-like Growth Factor-I (IGF-I). The biological activity of IGF-I is modulated by IGFbinding proteins (IGF-BPs) and at the same time, the availability of IGF-BPs may be regulated by the proteolytic activity of some metalloproteinases (MMPs). The aim of the present study was to compare the amounts of IGF-I and IGF-BPs in relation to the activity of MMP-9 in serum and knee synovial fluid from patients with Lyme arthritis (LA) and post-traumatic damage (PTD). Material and methods: Serum and synovial fluids were taken from knee joints of patients with PTD and LA. ELISA (for IGF-I assay), polyacrylamine gel electrophoresis following Western immuneblotting (for IGF-I and IGF-BPs expression), and zymography (for metalloproteinases detection), were used. Results: The concentration of IGF-I in serum and synovial fluid from LA patients were significantly lowered in comparison to PTD patients. Interesting, the synovial fluid /serum ratio of IGF-I concentrations was also lower in LA patients. Low expression IGF-BP3 and high activity of MMP-9 were detected in the LA synovial fluid. Conclusions: The high proteolytic activity of MMP- 9 results in a cleavage of both IGF-I and IGF-BP3 causing a decrease in content of these substances in LA synovial fluid. In addition, the reduction in IGF and IGF-BP amounts may affect the repair processes in joint tissues of LA patients. The low concentration of IGF-I and IGF-BP3 slows down the repair processes in the joint tissues of LA patients.

5

Strongyloides papillosus: a metalloproteinase secreted by the invasive larvae

75%

Moczon T.

Wiadomości Parazytologiczne

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1998

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tom 44

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nr 3

6

Induced sputum metalloproteinases and their inhibitors in relation to exhaled nitrogen oxide and sputum nitric oxides and other inflammatory cytokines in patients with chronic obstructive pulmonary disease

63%

Ziora D. , Dworniczak S. , Kozielski J.

Journal of Physiology and Pharmacology. Supplement

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2008

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tom 59

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nr 6

809-817

7

Gene expression profile analysis in laryngeal cancer by high-density oligonucleotide microanalysis

63%

Markowski J. , Oczko-Wojciechowska M. , Gierek T. , Jarzab M. , Paluch J. , Kowalska M. , Wygoda Z. , Pfeifer A. , Tyszkiewicz T. , Jarzab B. , Niedzielska I. , Borgiel-Marek H.

Journal of Physiology and Pharmacology. Supplement

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2009

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tom 60

|

nr 1

57-63

EN

The assessment of gene expression profile in laryngeal cancer shall allow to implement molecular biology methods in diagnostics, as well as in prognosis of the course of disease. Thus, it may influence the choice of the most optimal decisions in regards to the method of treatment, extent of surgical procedure, or the necessity of adding post-operative radiotherapy. The aim of the project was to analyse the gene expression profile of laryngeal cancer using oligonucleotide microarrays, aiming to derive novel molecular markers for that carcinoma. The study comprised a group of 14 patients (12 males and 2 females) with squamous cell laryngeal carcinoma, diagnosed and surgically treated between 2005 – 2007 in the ENT Department of the Silesian Medical University in Katowice, Poland. RNA was isolated from frozen tissue fragments. To assess gene expression profile, high density oligonucleotide microarrays (Affymetrix U 133 Plus 2.0) were applied, with over 54 thousand probesets for over 47 thousand transcripts. Four genes, previously not assesed in diagnostic context in laryngeal carcinoma, seemed to be valuable markers of that neoplasm. These are: metalloproteinase ADAM12, cycline-dependent kinase 2 - CDK2, kinesine 14 - KIF14, suppressor 1 of checkpoint - CHES1.

8

Endometriosis — insights into a multifaceted entity

63%

Amalinei C. , Pavaleanu I. , Lozneanu L. , Balan R. , Giusca S.-E. , Caruntu I.-D.

Folia Histochemica et Cytobiologica

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2018

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tom 56

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nr 2

9

FGF binding by extracellular matrix components of Wharton's jelly

63%

Malkowski A. , Sobolewski K. , Jaworski S. , Bankowski E.

Acta Biochimica Polonica

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2007

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tom 54

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nr 2

EN

Our earlier paper has reported that Wharton's jelly is a reservoir of several peptide growth factors, including acidic and basic fibroblast growth factors (aFGF and bFGF, respectively). Both can be extracted by buffered salts solutions in the form of high molecular mass complexes, probably with a component(s) of the extracellular matrix. Both aFGF and bFGF from such extracts hardly penetrate 10% polyacrylamide gels during electrophoresis. Pre-treatment of Wharton's jelly with hyaluronidase slightly increased the extractability of aFGF, but did not affect the extractability of bFGF. In contrast, the pre-treatment of tissue homogenate with bacterial collagenase (2000 U/ml, 37°C, 18 h) increased the extractability of bFGF. The presence of β-mercaptoethanol in the extracting solutions increased the extractability of both FGFs, but did not release FGFs in their free form, despite reducing the molecular mass of the FGF-containing complexes. We conclude that both aFGF and bFGF are bound through disulphide bonds to a protein component of Wharton's jelly. We propose that ground substance composed mainly of collagen fibrils and hyaluronate molecules, which surrounds the cells of Wharton's jelly, prevents the access of the extracting solution to aFGF and bFGF. Although hyaluronate and collagen do not bind aFGF or bFGF directly, they may constitute a barrier which prevents the dispersion of FGFs in Wharton's jelly. Thus, the high concentration of FGFs around the cells of Wharton's jelly may facilitate the interaction of these factors with membrane receptors, thereby resulting in stimulation of cell division and differentiation, as well as of the synthesis of extracellular matrix components.

10

Involvement of MMPs in delayed neuronal death after global ischemia

63%

Zalewska T. , Ziemka-Nalecz M. , Sarnowska A. , Domanska-Janik K.

Acta Neurobiologiae Experimentalis

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2002

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tom 62

|

nr 2

11

The effects of the initial treatment phase and of adjunctive low-dose doxycycline therapy on clinical parameters and MMP-8, MMP-9, and TIMP-1 levels in the saliva and peripheral blood of patients with chronic periodontitis

63%

Gorska R. , Nedzi-Gora M.

Archivum Immunologiae et Therapiae Experimentalis

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2006

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tom 54

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nr 6

12

Interferon-gamma regulates alpha-2-macroglobulin and alpha-1-antichymotrypsin expression on the pretranslational level in HepG2 cells

63%

Kordula T. , Rose-John S. , Heinrich P. C. , Koj A.

Folia Histochemica et Cytobiologica

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1992

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tom 30

|

nr 4

13

A metalloproteinase secreted by the infective larvae of Strongyloides papillosus [Nematoda]

63%

Moczon T.

Acta Parasitologica

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1999

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tom 44

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nr 3

EN

Infective larvae of Strongyloides papillosus, freshly isolated from faeces of experimentally infected rabbits, secreted a collagenolytic metalloproteinase from their oesophageal glands. The enzyme hydrolyzed azocoll at the optimal pH of 8.4 and exhibited a very low activity towards azocasein and azoalbumin at the optimal pH 6.0 and 8.0, respectively. No degradation of elastin-orcein and keratinazure was observed at the pH range of 7.2-10.0. Under histochemical conditions the proteinase hydrolyzed N-acetyl-L- methionine-l-naphthylester at optimal pH 6.8, whereas other synthetic, N-blocked aminoacyl or peptidyl substrates bearing such P₁ amino acids as L-Ala, L-Phe, L-Arg, L-Leu, and L-Lys, were not hydrolyzed. The enzyme was sensitive to refrigeration and underwent inactivation during lyophilization. Unlike most proteinases of other families, the metalloenzyme secreted by S. papillosus larvae was relatively resistant to the inhibitory action of inorganic zinc salts, the decline in the activity in the presence of 1 mM ZnSO₄ was as low as 20%. The organic mercurial pHMB, the nonionic detergent Triton X-100, and calcium ions enhanced the proteinase activity, whereas the cationic detergent cetyltrimethylammonium bromide, the anionic detergent SDS, and the thiol compound dithioerythritol were mild inhibitors. Zinc-chelating compounds 1,10-phenanthroline, N-blocked- L-Pro-L-Leu-Gly hydroxamate, N-blocked L-Pro-L-Leu-L- Ala hydroxamate, and N-carboxymethyl-L-Phe-L-Leu were strong inhibitors, whereas specific inhibitors of serine, cysteine and aspartyl proteinases were without effect on the activity of the larval proteinase. The secretion expelled from the mouth of the larvae avidly absorbed the cationic dye toluidine blue (0.001%) at pH 5.5 and the resulting black complex was water insoluble, thus indicating the presence of a strongly anionic glycoprotein in the secretion, if not an acid glycoprotein nature of the proteinase.

14

Plasma and plasma cell surface measurements of urokinase plasminogen activator system in multiple myeloma patients

63%

Dmoszynska A. , Cioch M. , Walter-Croneck A. , Jawniak D. , Hus I. , Rolinski J.

Bulletin of the Veterinary Institute in Puławy. Supplement

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2002

15

Expression of matrix metalloproteinase 9 in pancreatic ductal carcinoma is associated with tumor metastasis formation

63%

Pryczynicz A. , Guzinska-Ustymowicz K. , Dymicka-Piekarska V. , Czyzewska J. , Kemona A.

Folia Histochemica et Cytobiologica

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2007

|

tom 45

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nr 1

16

Lentiviral vectors in gene therapy: Their current status and future potential

51%

Escors D. , Breckpot K.

Archivum Immunologiae et Therapiae Experimentalis

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2010

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tom 58

|

nr 2

107-119

17

Histopathologic and immunohistochemical lesions in liver of mink infected with Aleutian disease virus

51%

Valdovska A. , Pilmane M.

Polish Journal of Veterinary Sciences

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2011

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tom 14

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nr 1

EN

Parvovirus of Aleutian disease causes mainly damage to kidneys, but immune complexes deposition and damage may occur also in other organs. In mink farms of Latvia the liver dystrophy or hepatic lipidosis of mink is widely distributed. The goal of this study was to examine probability of liver damage and regeneration of mink infected with Aleutian disease virus. Liver injury was assessed histologically. The mink liver demonstrated inflammation of liver parenchyma and foci of fatty liver. In immunohistochemistry, during liver regeneration the matrix metalloproteinases MMP-9, vascular endothelial growth factor and β-defensin 2 expressions were lower, but MMP-2 and nerve growth factor receptor p75 expression was increased.

18

Proteomic analysis of tumor tissue in CT-26 implanted BALB/C mouse after treatment with ascorbic acid

51%

Lee J. , Lee G. , Park J. H. , Lee S. , Yeom C.-H. , Na B. , Park S.

Cellular and Molecular Biology Letters

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2012

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tom 17

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nr 1

EN

Tumor establishment and penetration consists of a series of complex processes involving multiple changes in gene expression and protein modification. Proteome changes of tumor tissue were investigated after intraperitoneal administration of a high concentration of ascorbic acid in BALB/C mice implanted with CT-26 cancer cells using two-dimensional gel electrophoresis and mass spectrometry. Eighteen protein spots were identified whose expression was different between control and ascorbic acid treatment groups. In particular, eukaryotic translation initiation factor 3 subunit 1, nucleophosmin, latexin, actin-related protein 2/3 complex subunit 5, M2-type pyruvate kinase, vimentin, tumor protein translationally-controlled 1, RAS oncogene family Ran, plastin 3 precursor, ATPase, Rho GDT dissociation inhibitor β, and proteasome activator subunit 2 expression were quantitatively up-regulated. The increase in the level of these proteins was accompanied by an increase in mRNA level. The cytoskeleton protein actin, vimentin, and tumor protein translationally-controlled 1 showed quantitative expression profile differences. A change in actin cytoskeleton distribution, functionally relevant to the proteome result, was observed after treatment with ascorbic acid. These results suggest a previously undefined role of ascorbic acid in the regulation of cytoskeleton remodeling in tumor tissues.

19

Estrogen receptor beta participate in the regulation of metabolizm of extracellular matrix in estrogen alpha negative breast cancer

51%

Lesniewska M. , Miltyk W. , Swiatecka J. , Tomaszewska M. , Kuzmicki M. , Palka J. , Wolczynski S.

Folia Histochemica et Cytobiologica

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2009

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tom 47

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nr 5

20

Strong association between fibronectin accumulation and lowered cathepsin B activity in glomeruli of diabetic rats

51%

Wyczalkowska-Tomasik A. , Bartolomiejczyk I. , Gornicka B. , Paczek L.

Journal of Physiology and Pharmacology

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2012

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tom 63

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nr 5