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The purpose of the survey was to assess the prevalence of BHV-1, PI-3, BHV-4, BRSV and BVD in the bovine respiratory tract. Sera were taken from cattle of four districts located in the south-western part of Poland: 349 sera were collected from cows, 183 from heifers and 27 from bulls with no clinical signs of the disease. The findings revealed higher indices of infection caused by the viruses in the group of cows as compared with the one of heifers, and the highest percentage of infections due to PI-3 virus in all groups of the animals. With respect to viruses and districts under study, the following indices of infections were found: BHV-1 from 6.2 to 44.4 per cent, PI-3 from 32.7 do 69 per cent, BHV-4 from 13.9 to 40 per cent, BRSV from 8.5 to 44.4 per cent and BVD from 8.5 to 27.7 per cent. The results indicate significant differences in respect to virus infections caused mainly by the home and international market of cattle. It is worth mentioning that mixed infections were observed more often than monoinfections.
Powszechnie stosowane kryterium rozpoznawania przypadków toksoplazmozy ostrej na podstawie obecności swoistych IgM w surowicy badanej osoby bywa zawodne. Dokładniejszy sposób określania terminu zarażenia toksoplazmą polega na oznaczeniu awidności swoistych przeciwciał klasy IgG. Metoda oznaczania awidności powinna być wykorzystywana podczas badania ciężarnych podejrzanych o inwazję pierwotną, umożliwia bowiem niejednokrotnie wykluczenie ryzyka zarażenia płodu.
A total of 37 serum samples from pregnant women were examined for the avidity of toxoplasma IgG antibodies using commercially available enzyme immunoassay (Vidas Toxo TgG Avidity, bio Merieux). Low values of the avidity index were found for 4 samples and borderline ones were found in further 3 cases. Out of 19 serum samples showing the presence of IgG and IgM toxoplasma antibodies, 13 had high avidity index, including 9 samples from women in the first trimester of pregnancy. The results of examinations in these 9 cases allowed to exclude the possibility that toxoplasma infection was acquired during gestation. It is to be concluded that determinations of antitoxoplasma IgG avidity should become routine in every pregnant woman positive in the test for specific IgM antibody.
It bas been suggested to change the present way of serological diagnosis of echinococcosis in Poland which utilizes immunoelctrophoresis for confirmation of positive results in a screening test. Diagnostic procedure should be indicated with a sensitive test, such as indirect haemagglutination or immunoenzymatic assay, and every positive result should be verifies later using a Western-blot method in order to detect the most specific reaction with 8 kDa fraction of Echinococcus antigen. Further diferentiation of the infecting species may be accomplished with the aid of Em2 plus ELISA test which detect antibodies speeific for E. multilocularis.
The purpose of this Work was to analyse results of serologie examinations and complaints reported by patients who were operated on echinococcal liver cysts. The analysis was conducted on patients who were qualified to surgical removal of echionococcal liver cysts. Persons who were analysed answered the questionaires and 11 of them send sera for serologie examination. The questionaires consisted of questions about time since operation, complaints before and after operation abdomen pains, distensions, vomits, exanthema, weakness, headaches). Results showed that patient were from 1 to 6 years after operation. Among 11 who send sera 18% results were negative (2 and 6 years after operation) and 82% was positive. Analysis of 15 questionnaires showed that majority of patients still had the complaints that were present before operation.
The aim of the study was to assess the seroprevalence of Equine rhinitis B viruses (ERBV) in the horse population of the south-eastern part of Poland. Selected horse farms, including breeding farms, stallion herds, purchasing centers and riding clubs were included in the studies. Blood samples were taken from 650 adult horses and foals of different age groups from 23 farms. Commercial ELISA test (Equivir IgG ELISA, Tridelta Development Limited, Ireland) has been used in the studies. On average specific antibodies to ERBV were found in 70.5% of the animals examined. The percentage of positive results of the serological survey was diverse in particular horse farms and ranged from 37.5% to 100% of the animals. It was demonstrated that the farm type and sex of horses did not influence the serological results, while the number of horses in the farm significantly influenced the serological results (P < 0.05).
The article presents the results of serological surveys of brucellosis conducted in 1998 on cattle, pigs, sheep, goats, hares, wild boars and dogs. No positive sero-reagents among the pig population were ascertained. The rate of positive results in cattle was established as 0.00098. When B. abortus antigen was used, all serum samples from sheep and goats reacted negatively. When B. ovis antigen was used, 0.91% of sheep sera were positive. The presence of anti-Brucella antibodies was demonstrated in hares, wild boars and dogs.
The aim of the study was to perform a preliminary evaluation of the ELISA test for the diagnosis of bovine tuberculosis in Polish conditions. The Idexx kit of immunoassays for detecting the presence of antibodies specific for Mycobacterium bovis in samples of cattle’s blood serum and plasma was used to examine a total of 50 serum samples from clinically healthy animals, 44 sera from cows with previous positive result of tuberculin tests, but negative in microbiological examination, 25 sera from animals from whose tissues strains of Mycobacterium bovis had been isolated (including 10 sera from cows with characteristic tuberculous lesions and 15 sera from animals without these lesions), and 16 serum samples from animals reacting positively for Johne’s disease. On the basis of the results of these examinations, the specificity of the test was determined at 100% and sensitivity at 90%. There were no cross-reactions, and the results indicate that the ELISA Idexx kit may be used as an important diagnostic tool in the diagnosis of bovine tuberculosis.
The purpose of the study was to carry out serological examinations in foxes originating from farms with unsatisfied reproduction results located in the Silesia, Lublin, Mazowsze, Pomorze, Mazury and Wielkopolska districts. Moreover, the same examinations were conducted in reference to sera of mink with bad reproduction findings. Out of 435 sera from 17 fox farms, HI antibodies were found in 28.6 per cent. Specific antibodies were also recorded in 2 of 3 mink farms. Of 132 sera, the antibodies were found in 72 samples (54.5 per cent). In addition, from 19 samples of faeces, homogenized internal organs of 7 foetuses and the uterus of a pregnant mink, a parvovirus was isolated (their HA=640-2560). A marked distribution of parvovirus infections in foxes indicates that vaccination should be carried out in the animals before copulation. However, others reasons, such as unsatisfactory nutrition, could influence bad reproduction results.
Исследования провели с применением теста AGID. В 2 коровниках, насчитывающих 75 и 47 коров, получили в первом исследовании 10% и 8% положительных серологических результатов. Дальнейшие исследования выполняли в 4—6-месячных интервалах. Животных, зараженных BLV, удаляли немедленно из коровника. На второй год освобождения стада от ЭЛС у всех животных получили отрицательные результаты. В коровниках молочного скота при проценте инфекций до 10% можно в течение 2 лет посредством систематических исследований довести до освобождения стада от ЭЛС при условии его пополнения телками, освободными от лейкоза. Вариант освобождения крупных коровников от ЭЛС, состоящий в изоляции инфицированных коров в выделенном секторе того же самого коровника, осуществляли в хозяйстве насчитывающем 377 молочных коров. В первом исследовании получили 22% положительных серологических результатов. В очередных контрольных исследованиях обнаруживали новые инфекции BLV у коров в излечиваемых секторах. В течение 4 лет освободили этим путем коровник от ЭЛС.
The examinations using the AGID test were carried out on two cowsheds comprising 75 and 47 cows, respectively. The first testing revealed positive reactors in 10% of animals and 8% in the second cowsed. Further examinations were conducted at intervals of 4—6 months and the animals with positive seroconversion were removed from the premises. In the second year no animal showed the presence of antibody against EBL virus. The authors conclude that in cowsheds comprising milking cattle and in which not more than 10% of animals is infected with EBL virus, it is possible to release cowsheds from EBL within two years provided that cows are tested regularly and a cowshed is completed by heifers free from EBL. In case of large farms comprising hundreds animals the situation is much more complicated: e.g. on a farm containing 377 cows the first examination discovered the presence of specific antibodies in 22% of animals. All the milking cows were haused in the same premises; though the infected animals were in isolation nevertheless new infections were found in repeated examinations. At last after four vears the farm was free from EBL that was reached by elimination the seropositive animals.
The objective of this study was to analyze and estimate the epizootic situation of Aujeszky's disease (AD) in Wielkopolska voivodeship the biggest pig producing region of Poland in the years 2001-2005. Results of serological tests performed independently in the Regional Veterinary Diagnostic Laboratory (RVDL) in Poznañ (the capitol of Wielkopolska voivodeship) and in the Department of Swine Diseases of the National Veterinary Research Institute (NVRI) in Pu³awy are shown. A total number of 3584 pig sera from 480 pig farms were tested in RVDL Poznañ and 15 910 swine serum samples from 331 pig holdings were examined in NVRI Pu³awy. Serological monitoring studies revealed the presence of AD virus (ADV) antibodies in 9.2% and 1.54% pig sera, respectively. The higher percentage of positive serum samples detected by RVDL Poznañ than by NVRI Pu³awy was probably due to the fact that the sera tested by RVDL were taken from pigs with some health problems and that ones examined by NVRI came mostly from pigs from farms with a high health status (e.g. breeding and/or reproduction farms). On the other hand it is worth noticing that the seropositive pigs were found in 15.6% (RVDL) and 11.67% (NVRI) of the tested farms. From the results obtained it seems that the epizootic situation of AD was quite stable in the years 2001-2005, and the pig population in Wielkopolska voivodeship is not heavily infected with ADV. These findings should be taken into account before an AD eradication program will be implemented in this region of Poland.
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