This paper discusses the biosorption in a multi-cation system as an example of advances in the method of ions removal/binding to the biomass. Biosorption experiments were conducted on Chlorella kessleri biomass. The ions used in the experiment were Co(II), Cu(II), Mn(II) and Zn(II) and had the following configurations: binary, ternary and quaternary system. Also, the effect of the following anions Cl-, NO3-, SO42- on the biosorption process in the quaternary system (initial concentration of each ion was 0−300 mg L-1) was examined. The affinity order determined in the experiment was as follows: Cu(II)>Zn(II)>Mn(II)>Co(II). At higher concentrations of Cu(II) cations, the strong competition effect between Cu(II) cations with the remaining cations was observed. The modified Langumir competition model was proposed to support the biosorption method in the description of the experimental data of inhibited metal ions biosorption. After the influence of anions was examined, the highest total biosorption capacity was obtained for 1:1:2 system (Cl-: NO3- : SO42-).
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In this work for disposal of the biologically hard decomposed pollutant Benzo[a]Pyrene (BaP) photooxidation Chlorella kessleri was used. The simulation model system under the different experimental conditions (varying biomass and light intensity) was evaluated. For quantitative analysis of the decrease in BaP, GC/MS technique was used. The highest degradation efficiency was achieved in the case of biomass from the culture of live algae (29%) and light intensity at level of 13.5 W m−2. When the dry biomass was used, degradation under the same conditions was lower because of lack of enzymatic activity in the system.
Metal sorption capacity of Chlorella kessleri and two strains of cyanobacteria (Aphanocapsa sp., Anabaena flos-aquae) was studied. It was found that among studied organisms, dead cells of C. kessleri were the most effective sorbents of Pb, Cu, Cd and Zn. They displayed the highest cation-exchange (Cd2+/H+) capacity and bound much more Cd and Zn at pH 7 than at pH 4. The optimum pH for sorption of Pb and Cu was 6. At pH 6, dead cells of C.kessleri could bind maximally about 37 mg Cd, 38 mg Zn, 21 mg Cu and 70 mg Pb per g of dry weight. Generally, algal dead cells (0.3 g dry wt dm^3) removed 50-70% of the metal ions from 0.01 mM solutions. A surplus of calcium and magnesium caused a slight decrease of Pb, Cu, Cd and Zn sorption.
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