Wyniki wyszukiwania - Biblioteka Nauki

1

Activity of resveratrol on the influence of aflatoxin B1 on the testes of Sprague dawley rats

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Omur A. D. , Yildirim B. , Saglam Y. S. , Comakli S. , Ozkaraca M. , Omur A. D. , Yildirim B. , Saglam Y. S. , Comakli S. , Ozkaraca M.

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nr 2

2

Sperm epigenetic profile and risk of cancer

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Wdowiak A.

Journal of Pre-Clinical and Clinical Research

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2014

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tom 08

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nr 2

EN

Introduction and objective. The integrity, stability and composition of sperm chromatin are of great importance in the fertilizing potential of male gametes and their capacity to support normal embryonic development. In this study, the author presents the current state of knowledge about the sperm epigenetic profile and risk of cancer. Abbreviated description of the state of knowledge. The obtaining of pregnancy and the state of health of the baby depends on the quality of the genetic material of both the female and the male. Health behaviours and environmental factors directly affect the quality of sperm, as well as the human egg cell and, consequently, on the reproductive capabilities, the course of pregnancy and the state of the newborn. There exist two thoroughly investigated epigenetic modifications: DNA methylation and histone modifications. The process of DNA methylation can be also a fundamental factor contributing to the development of cancer, where epigenotype undergoes significant modifications. When considering numerous DNA aberrations in the male gamete, the most commonly encountered is DNA fragmentation, particularly in infertile subjects. Surprisingly, an intracytoplasmatic sperm injection study of mice oocytes, using spermatozoa with a high DNA Fragmentation Index (DFI), revealed that a considerable percentage of adults born as a result of this method, showed a significant increase in the incidence of abnormal behavioural tests, malformations, cancer and signs of premature aging. Summary. The issue of assisted procreation raises the need to look for an appropriate treatment for males with sperm chromatin abnormalities. As a result, the fight against smoking addiction becomes the obvious necessity. Moreover, the reasonable solution nowadays seems to be supplementation with micronutrients and folic acid. It has been proved that the process of DNA fragmentation is a phenomenon that intensifies over time. Therefore, there should be a pursuance for, as close as possible, to the moment of ejaculation, application of semen to reproductive techniques. Finally, epigenetic changes are suspected of being one of the factors responsible for the deterioration of male sperm parameters observed in recent decades.

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Aktywność katalazy i stężenie żelaza w nasieniu ludzkim

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Kasperczyk A. , Horak S.

Annales Academiae Medicae Silesiensis

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2013

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tom 67

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nr 2

112-116

EN

INTRODUCTION The activity of antioxidant system enzymes in human semen might be age dependent, thus the quality of it may get worse. The aim of this study was to investigate the correlation between the age, catalase activity and iron concentration in seminal plasma and the influence of these factors on the quality of semen. MATERIALS AND METHODS Normospermic semen samples were obtained from 63 men (range, 22–37 years) and divided in two age groups: under 33 (group I) and over 33 years old (group II). The semen parameters, catalase activity and iron concentration were determined in seminal plasma. RESULTS We observed that in group II the iron concentration in seminal plasma increased but sperm motility (especially linear progressive motility) dropped with age. A lower semen volume showed a significant increase in catalase activity as well as iron concentration. Increasing catalase activity showed a significant positive relationship with better sperm quality. We found positive correlations between catalase activity and iron concentration. CONCLUSION Catalase has a protective effect on sperm cell membranes. Iron concentration in seminal plasma rises in an age dependent manner which may contribute to sperm cells damage.

PL

WSTĘP Aktywność enzymów wchodzących w skład układu antyoksydacyjego nasienia może w miarę starzenia organizmu ulec osłabieniu, co może być przyczyną obniżenia zdolności zapładniającej nasienia. Celem pracy było ustalenie, czy wraz z wiekiem zmieniają się aktywność katalazy i stężenie żelaza w nasieniu ludzkim i jak zmiany te wpływają na jego jakość. MATERIAŁ I METODY Materiał badany stanowiło nasienie o prawidłowej morfologii pobrane od 63 mężczyzn w wieku od 28 do 37 lat (grupa I – do 33 roku życia; grupa II – powyżej 33 roku życia). Wykonano badanie morfologiczne nasienia, oznaczono aktywność katalazy i stężenie żelaza w plazmie nasiennej. WYNIKI Stwierdzono, że wraz z wiekiem obniża się liczba plemników ruchliwych, zwłaszcza o ruchu linearnym, natomiast zwiększa się stężenie żelaza. Wraz ze spadkiem objętości nasienia wzrasta aktywność katalazy i stężenie żelaza, zaś wzrostowi aktywności katalazy towarzyszy wzrost stężenia ruchliwych plemników. Wykazano ponadto dodatnią korelację pomiędzy aktywnością katalazy a stężeniem żelaza. WNIOSKI Katalaza wykazuje działanie ochronne na błonę komórkową plemników. Stężenie żelaza w plazmie nasienia wzrasta u mężczyzn wraz z wiekiem, co może przyczyniać się do uszkodzenia plemników.

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Air pollution and semen parameters in men seeking fertility treatment for the first time

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Wdowiak A. , Wdowiak E. , Bień A. , Bojar I. , Iwanowicz-Palus G. , Raczkiewicz D.

International Journal of Occupational Medicine and Environmental Health

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2019

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tom 32

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nr 3

387-399

EN

Objectives The purpose of this paper was to analyze the relationship between the selected chemical air pollutants found in the Lublin Province and the semen parameters of men seeking fertility treatment for the first time. Material and Methods The study involved an analysis of semen sample test results obtained from male patients first reporting for fertility treatment in reproductive health centers in the Lublin Province, Poland. The data set comprises semen parameters of 13 148 men, and the number of samples in the reference period was 255–769 annually. Data on air pollution were obtained from the website of the Polish General Environmental Inspectorate and included selected chemical air pollutant levels, i.e., NO₂, SO₂, O₃, and PM₁₀. Results The mean PM₁₀ levels in the air increased, on average, by 0.65 μg/m₃ annually in 2000–2015 (p = 0.029); the mean levels of O₃, NO₂, SO₂ did not change significantly in the analyzed period. There were increasing trends in the mean sperm density and total sperm count of the subjects in 1992–2015 (p < 0.001). The mean percentage of sperm with normal morphology significantly decreased in the subsequent analysis periods: 1992–1998 (p = 0.001); 1999–2009 (p < 0.001); 2010–2015 (p = 0.001). A significant negative correlation was found between the ozone levels in the air in the Lublin Province and the percentage of sperm with normal morphology (r = –0.8311, p = 0.040). Conclusions Exposure to ozone in the air contributes to decreased percentages of sperm with normal sperm morphology. Over the years, there was an increasing trend in sperm density in the men first reporting for fertility treatment, and a decreasing trend in the percentage of sperm with normal morphology. Int J Occup Med Environ Health. 2019;32(3):387–99

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Sperm quality and DNA integrity of coke oven workers exposed to polycyclic aromatic hydrocarbons

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Jeng H. A. , Pan C.-H. , Chao M.-R. , Chiu C.-C. , Zhou G. , Chou C.-K. , Lin W.-Y.

International Journal of Occupational Medicine and Environmental Health

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2016

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tom 29

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nr 6

915-926

EN

Objectives The objective of this study was to assess sperm quality and deoxyribonucleic acid (DNA) integrity of coke oven workers exposed to polycyclic aromatic hydrocarbons (PAHs) as compared to control subjects. Material and methods The coke oven workers (N = 52) and administrative staff (N = 35) of a steel plant served as the exposed and control groups, respectively. Exposure to PAHs was assessed by measuring 1-hydroxypyren. Analysis of sperm quality (concentration, motility, vitality, and morphology) was performed simultaneously with sperm DNA integrity analysis, including DNA fragmentation, denaturation, bulky DNA adducts, and 8-oxo-7,8-dihydro-2’-deoxyguanosine (8-oxo-dGuo). A questionnaire was conducted to collect demographic and potential confounding data. Results The coke oven workers had lower percentages of sperm motility, vitality and normal morphology than the control group, but the difference was not significant. For DNA integrity, the coke oven workers had significantly higher concentrations of bulky DNA adducts and 8-oxo-dGuo than the control subjects (p = 0.009 and p = 0.048, respectively). However, DNA fragmentation percentages did not significantly increase as compared to those in the subjects from the control group (p = 0.232). There was no correlation between sperm quality parameters and DNA integrity indicators. Conclusions Occupational exposure of the coke oven workers to PAHs was associated with decreased sperm DNA integrity. Int J Occup Med Environ Health 2016;29(6):915–926

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Royal jelly protection on flunixin meglumine-induced spermiotoxicity and testicular degeneration in mice

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Temamoğulları F. , Aral F. , Yılmaz R.

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nr 3

7

Influence of splicing mutation within the lysophosphatidic acid receptor 1 gene (LPAR1) on semen quality in Holstein-Friesian bulls

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Kamiński S. , Hering D. M. , Kordan W. , Lecewicz M. , Sazanov A.

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8

Missense mutation within cystic fibrosis transmembrane conductance regulator (CFTR) gene is associated with selected parameters of the frozen-thawed spermin Holstein-Friesian bulls

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Kaminski S. , Hering D. M. , Kordan W. , Lecewicz M. , Kaminski S. , Hering D. M. , Kordan W. , Lecewicz M.

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nr 2

9

The effect of breed on freezability of semen of fancy fowl

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Siudzinska A. , Lukaszewicz E.

Animal Science Papers and Reports

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2008

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tom 26

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nr 4

EN

A simple cryopreservation method described in 1995 by Tselutin et al. was used for freezing the semen of four fancy fowl breeds: White Crested Black Polish (WCBP), Greenleg Partridge (GP),Italian Partridge (IP) and Black Minorca (BP). The differences in quality (ejaculate volume,osmotic pressure, sperm concentration and morphology) of fresh semen between evaluated breeds were observed, as well as the differences in semen freezability. The freezing-thawing process caused significant (P≤0.01) decrease in percentage of live, normal spermatozoa, with coincident increase in percentage of dead spermatozoa and spermatozoa with acrosome defect. In relation to the fresh semen, the number of live, normal spermatozoa that survived cryopreservation procedure constituted 18.1% in WCBP, 25.1% in GP, 26.2% in IP and 33.6% in BM semen.

PL

Badano przydatność prostej metody mrożenia opracowanej i opisanej w 1995 r. przez Tselutina i współautorów do zamrożenia nasienia kogutów czterech amatorskich ras kur: czubatki polskiej (White Crested Black Polish – WCBP), zielononóżki kuropatwianej (Greenleg Partridge – GP), włoszki kuropatwianej (Italian Partridge – IP) i minorki czarnej (Black Minorca – BM). Stwierdzono różnice w jakości nasienia świeżego (objętość ejakulatów, ciśnienie osmotyczne, koncentracja i morfologia plemników) między kogutami ocenianych ras, a także różnice między rasami w podatności nasienia na mrożenie. Proces ekwilibracji nasienia w obecności 6% DMA oraz jego zamrożenie-rozmrożenie powodowały istotny (P≤0,01) spadek liczby plemników żywych, prawidłowo ukształtowanych, przy równoczesnym wzroście liczby plemników martwych lub z uszkodzonym akrosomem. Bez względu na rasę kogutów, mrożenie nasienia zastosowaną metodą powodowało spadek liczby plemników żywych prawidłowo ukształtowanych, których udział w stosunku do udziału w nasieniu świeżym stanowił w przypadku czubatki polskiej 18,1%, zielononóżki kuropatwianej 25,1%, włoszki kuropatwianej 26,2% oraz 33,6% w przypadku minorki czarnej.

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Self-association of Chaetopterus variopedatus sperm histone H1-like. Relevance of arginine content and possible physiological role

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Salvati D. , Conforti S. , Conte M. , Matassa D. S. , Fucci L. , Piscopo M.

Acta Biochimica Polonica

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2008

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tom 55

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nr 4

701-706

EN

Self-association of histones H1 from calf thymus and from sperm of the marine worm Chaetopterus variopedatus was studied on native and glutaraldehyde cross-linked molecules by PAGE and by salt-induced turbidity measurements. Multiple polymers were generated by native sperm histone H1-like after glutaraldehyde cross-linking while the same treatment on its lysine- or arginine-modified derivatives and on somatic histone H1 failed to induce polymerization. This result suggests the relevance of arginine content in the formation of histone H1-like polymers particularly because Chaetopterus variopedatus and calf thymus histones H1 have similar content of lysine but different K/R ratio (2 and 15, respectively). Salt-induced turbidity experiments confirmed the high tendency of sperm histone H1-like to form oligomers, particularly in the presence of phosphate ions. Native PAGE analysis in the presence of phosphate supported this hypothesis. The reported results suggest that phosphate ions connecting lysine and arginine side chain groups contribute to the interaction of sperm histone H1-like with DNA in chromatin and mplay a key role in organization and stabilization of the chromatin higher order structures.

11

Evaluation of spermiogram in domestic pigs

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Kondracki S. , Wysokinska A. , Banaszewska D. , Wozniak E.

Reproductive Biology

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2006

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tom 06

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nr Suppl.2

12

Kinetyka transportu subpopulacji plemników in vitro w zmiennym polu magnetycznym z zakresu ELF

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Jaroszyk F. , Nawrocka H. , Warchoł W. , Włodarczyk D.

Acta Bio-Optica et Informatica Medica. Inżynieria Biomedyczna

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2004

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tom Vol. 10, nr 3-4

91-99

PL

Badano wpływ zmiennego pola magnetycznego typu M^P, i M^P^, generowanego w zakresie ELF (extremly low frequency, 0-300 Hz) przez urządzenie Viofor JPS, na kinetykę transportu subpopulacji plemników w stanie in vitro. Porównano kinetykę transportu subpopulacji plemników poddawanych działaniu pól magnetycznych z kinetyką transportu subpopulacji plemników w stanie in vitro stanowiących grupę kontrolną, niepoddaną działaniu pól magnetycznych.

EN

The influence of magnetic field on sperm subpopulation motility in vitro was examined. The magnetic field type M|P, or M|P, was generated by Viofor JPS apparatus in the range of ELF (extremly low frequency, 0-300 Hz). The results were compared with control subpopulation not exposed to the magnetic field.

13

Assessment of the morphometry of heads of normal sperm and sperm with the Dag defect in the semen of Duroc boars

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Banaszewska D. , Andraszek K.

Journal of Veterinary Research

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2021

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tom 65

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nr 2

14

Studies on sperm biological value in cases of men infertility

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Kotwicka M. , Butowska W. , Filipiak K. , Skonieczna D. , Warchol J. B.

Folia Morphologica

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1998

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tom 57

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nr 1

15

Intracytoplasmic injection of cytometrically sorted bull sperm

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Skrzyszowska M. , Bochenek M. , Katska L. , Smorag Z.

Roczniki Naukowe Zootechniki

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2000

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tom 27

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nr 2

16

Movements of fish sperm flagella studied by high speed videomicroscopy coupled to computer assisted image analysis

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Cosson J. , Billard R. , Cibert C. , Dreanno C. , Linhart O. , Suquet M.

Polskie Archiwum Hydrobiologii

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1997

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tom 44

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nr 1-2

17

Evidence for aromatase localization in cytoplasmic droplet of human immature ejaculated spermatozoa

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Rago V. , Bilinska B. , Palma A. , Ando S. , Carpino A.

Folia Histochemica et Cytobiologica

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2003

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tom 41

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nr 1

18

Molecular targets for immunocontraception

86%

Stoyka B. , Domagala A. , Kurpisz M.

Archivum Immunologiae et Therapiae Experimentalis

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2003

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tom 51

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nr 1

19

Changes in the qualities of stallion semen after incubation with Streptococcus zooepidemicus

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Danek J. , Wisniewski E. , Krumrych W. , Dabrowska J.

Bulletin of the Veterinary Institute in Puławy

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1996

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tom 40

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nr 2

EN

The influence of ß-haemolytic Streptococcus organisms at concentrations of 100 x, 200 x, and 500 x 10³ cells/ml sperm incubated at 37°C for 60 min with stallion semen suspension on the motility, viability and morphology of the semen, and AspAT activity in seminal plasma was examined. S. zooepidemicus at a concentration of 500 x 10³ cells/ml exhibited a particularly unfavourable effect on semen. All parameters measured altered significantly after a 60 min incubation. The activity of AspAT also changed during a prolonged incubation of sperm with a suspension containing 500 x 10³ S. zooepidemicus/ml semen; after a 24 h incubation the activity of AspAT was enhanced by more than 38% in comparison to the initial value.

20

Effects of seminal plasma concentration on sperm motility and plasma and acrosome membrane integrity in chilled canine spermatozoa

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Pan C. , Wu Y. , Yang Q. , Ye J.

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nr 1