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Laparocholecystectomy was done on 4 cross-breed Large-White pigs, males and females of 20 kg b.w. under halotan general anesthesia. Peritoneal emphysema was created by carbon dioxide at a pressure of 10-12 mm Hg. The operation was performed using trocars of different diameters in four accesses. Biliary ductule and artery were closed by titanic staplers Endoclip TM - Auto Suture and then ruptured. There were no complications during the operation and in the post-operation phase. After the operation pigs woke up in a few hours and after 24 h they were fed balanced nutritional doses which were increased as body weight increased. After 5 months pigs gained 110-115 kg b.w. Laparocholecystectomy reduces operation trauma and enables quick recovery. The four spot peritoneal access made by trocar eliminates the possibility of many postoperative complications that could develop after classical laparotomy with a long abdominal incision.
The own method of the pancreas cannulation was introduced and the examinations were done on two groups of pigs, 10 animals each, for 14 days. In the 1st group an operation was performed in a way to be able to collected the whole of the secreted pancreatic juice. The twenty-four hour excretion of pancreatic juice was evaluated, taking into account the dynamic of excretion depending on the time of day and meals. On the 14th day-x-ray contrast examinations were performed to evaluate the pancreatic excretory ducts, and then, after slaughter, gross lesions were estimated and the pancreas samples were collected to estimate the structure of pancreatic parenchyma. In the 2nd group, a cannula of the own construction was used to collect pancreatic juice transported into the duodenum. The whole juice was not collected to prevent disturbances in the regulatory system of excretion. The apllied system therefore enabled an uncomplicated collection of pancreatic juice and duodenal content. The identical examinations as in group 1 were done in group 2. The applied method of the pancreas cannulation appeared to be effective, technically simple and non-invasive for pancreatic parenchyma. Transportation of pancreatic juice into the duodenum eliminates its hypersecretion that is of a great value for the condition of the pancreas after the experiment.
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