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Content available Micropropagation of Stryphnolobium japonicum
Observations of Japanese pagoda trees indicate that they undergo a full cycle of vegetative and generative development without self-renovation. The aim of this study was to obtain a successive media protocol propagation of Japanese pagoda tree by in vitro cultures. The effects of growth regulators were studied with reference to primary regeneration, shoot multiplication and rooting. As explants source were used the part of shoot of 90-year-oldtree. Explants were placed on MS (Murashige and Skoog 1962) basal medium with the addition of 6-benzylaminopurine – BAP (1.0–2.0 mg dm-3), thidiazuron – TDZ (0.1–0.3 mg dm-3) and indole-3-acetic acid – IAA (0.5–1.0 mg dm-3). BAP was the growth regulator which significantly increased shoot regeneration on initial explants. TDZ in turn, inhibited the formation of adventitious shoots and caused the explants which had been placed on the medium to die. The multiplication of the Japanese pagoda tree by in vitro cultures should be conducted on MS media with the addition of 0.5 mg dm-3 BAP, and they should be rooted on media with the addition of 0.3 mg dm-3 indole-3-butyric acid – IBA. It seems, that devising an efficient method of Japanese pagoda tree micropropagation is realistic.
Acidification and buffer capacity of bark in five deciduous Mediterranean plants were analyzed. The material originated from six stands with different levels of hovered dust, chiefly cement particles; its pH values were in general higher as compared with some other areas in Europe and Croatia. Quantitative data indicate a tendency of acidity to decrease in stands polluted with more than 200 µg/cm3 of hovered dust. Buffer capacities as a rule were higher against alkali and in particular species were ranging contrary to the range of ph values. The results are interpreted in favour of alkaline atmosphere influence.
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